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- 저자 : Jun-Biao Lu (검색결과 2 건)
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Jun Liu,Deng Feng Hong,Wei Lu,Ping Wu Liu,Qing Biao He,Guang Sheng Yang 한국유전학회 2008 Genes & Genomics Vol.30 No.6
Rs1046AB is a dominant genic male sterility (DGMS) line derived from a spontaneous mutant in Brassica napus. The sterility of this mutant was previously regarded as to be conditioned by the interaction of a dominant male sterility gene Ms and its non-allelic dominant restorer gene Mf (or Rf in previous reports). Recent genetic analyses, however, indicated that Ms and Mf may be allelic. In this research, we confirmed that a multiple allele model should be more appropriate to elucidate the heredity of DGMS line Rs1046AB. Based on this result, the present study emphasized on identifying DNA markers linked to Ms/Mf in an F2 population constructed by crossing Rs1046A with a double haploid (DH) restorer line (19514A). Twenty amplified fragment length polymorphism (AFLP) markers linked to the Ms/Mf locus were identified by combination of bulked segregant analysis (BSA) with AFLP technique. The target locus was detected to be co-segregating with the marker S05T05 and bracketed by two nearest markers, E14M01 and E01M02, with a genetic distance of 0.1 cM and 1.2 cM, respectively. Furthermore, we successfully converted two AFLP markers into sequence characterized amplified region (SCAR) markers. These findings provided direct molecular marker proofs on the inheritance model of this DGMS line, and the high density molecular markers linkage map around the Ms/Mf locus will be more informative for both marker-assisted selection (MAS) of elite male sterile lines and isolation of the Ms/Mf genes by positional cloning in future.
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Mineralization of calcium phosphate in reverse microemulsion
Xiang-Dong Kong,Xiao-Dan Sun,Jun-Biao Lu,Fu-Zhai Cui 한국물리학회 2005 Current Applied Physics Vol.5 No.5
In the present study, reverse microemulsion was prepared to regulate the mineralization of calcium phosphate. Calcium chlorideand sodium hydrogen phosphate aqueous solution were, respectively, dropped into the mixture of Span 80, Tween 80, andn-heptaneto get two kinds of emulsions.n-Buranol was used to adjust the emulsions to transparent state. Calcium phosphate was prepared byadding phosphate microemulsion to Calcium microemulsion. Heating method was used to induce the phase separation. After beingwashed with acetone and ethanol, the mineralized deposition were studied with scanning electron microscopy (SEM), X-ray dirac-tion (XRD) and transmission electron microscopy (TEM). The results indicated that the minerals in the deposition were mainlymonetite (DCPA) and the nanosized needle-like DCPA exhibited the preferential orientation along 200 direction..
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