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Song, Ji-Yoon,Cha, Joonseok,Lee, Joon,Roe, Jung-Hye AMERICAN SOCIETY OF MICROBIOLOGY 2006 EUKARYOTIC CELL Vol.5 No.11
<B>ABSTRACT</B><P>In the fission yeast <I>Schizosaccharomyces pombe</I>, the <I>pgr1</I><SUP><I>+</I></SUP> gene encoding glutathione (GSH) reductase (GR) is essentially required for cell survival. Depletion of GR caused proliferation arrest at the G1 phase of the cell cycle under aerobic conditions. Multicopy suppressors that restore growth were screened, and one effective suppressor was found to be the <I>trx2</I><SUP><I>+</I></SUP> gene, encoding a mitochondrial thioredoxin. This suggests that GR is critically required for some mitochondrial function(s). We found that GR resides in both cytosolic and organellar fractions of the cell. Depletion of GR lowered the respiration rate and the activity of oxidation-labile Fe-S enzymes such as mitochondrial aconitase and cytosolic sulfite reductase. Trx2 did not reverse the high ratio of oxidized glutathione to GSH or the low respiration rate observed in GR-depleted cells. However, it brought the activity of oxidation-labile Fe-S enzymes to a normal level, suggesting that the maintenance of Fe-S enzymes is a critical factor in the survival of <I>S. pombe</I>. The activity of succinate dehydrogenase, an oxidation-insensitive Fe-S enzyme, however, was not affected by GR depletion, suggesting that GR is not required for the biogenesis of the Fe-S cluster. The total iron content was greatly increased by GR depletion and was brought to a nearly normal level by Trx2. These results indicate that the essentiality of GR in the aerobic growth of <I>S. pombe</I> is derived from its role in maintaining oxidation-labile Fe-S enzymes and iron homeostasis.</P>
Electronic structure of p-type transparent conducting oxide CuAlO2
Salah Mohamed,Yoon Joonseok,El-Desoky Mohamed M.,Hussain Zahid,Ju Honglyoul,Mo Sung-Kwan 한국물리학회 2022 Current Applied Physics Vol.39 No.-
Copper-based delafossite oxides are excellent candidates for the p-type transparent conducting oxide (TCO), which is essential in realizing transparent semiconductor applications. Using angle-resolved photoemission spectroscopy (ARPES), we report the low-energy electronic structure of CuAlO2. We found that the band structure near the valence band top is characterized by hole bands with their maxima along the Brillouin zone boundary. Furthermore, the effective masses along the Γ–M and Γ–K directions were found to be (0.6 ± 0.1) m0 and (0.9 ± 0.1) m0, respectively, which impose an important benchmark against the existing band calculations.