건설업 안전경영시스템모델 개발

채준석,갈원모,손기상 안전경영과학회 2001 안전경영과학회지 Vol.3 No.2

Safety management cost, US$ one thousand billion has been input to the construction area from 1995 to 2000 but the management quality is still in the developing country level. The above mentioned increase of accident rate are attributed to the generous code regulation, net fit to the sense, varying with time change, but the more fundamental reason of this is no practical construction safety management system, up to now. Hardware-orienting operation for the system could be a main reason of this problem. In this paper, we developed a model for efficient construction safety system. It was found from the case study that the model result in high efficient with low cost.

안전관리 기법에 관한 실증적 연구 : 건설작업을 중심으로

채준석,갈원모,손기상 안전경영과학회 2000 안전경영과학회지 Vol.2 No.1

Many Safety training up to now has been done at site varying with their condition without any standardization or fundamental work related technique. In order to prevent any accidents from construction site, now as we all know, we have to approach on it with work based method. The authors have investigated a couple of construction site for collecting the ideas from site Engineers of this. Some of ideas analyzed with the above related data have been shown as conclusions.

한우에 감염된 Theileria sergenti merozoite의 순수분리와 genomic DNA probe에 관한 연구

채준석,이주묵,권오덕,채건상 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.17 No.-

To make the genomic DNA probe of Theileria sergenti, the merozoites were purified from bovine crythrocytes. The infected erythrocytes were lysed by Aeromonas hydrophila(Ah-1) hemolysin, and the parasites were isolated by ultracentrifugation on a Percoll discontinuous density gradient. For construction of a T sergenti genomic DNA library, T sergenti DNA was digested with PstI and the fragments were ligated into the PstI site of pUC19 before transformation of Escherichia coli JM83. Out of thousands of transformants obtained by transformation of E coli JM83 with the genomic library, three plasmids were chosen. The sizes of the inserted DNAs were 2.9kb(2.4kb and 0.5kb) in pKTS1, 4.3kb in pKTS2 and 1.5kb in pKTS3, respectively. The DNA fragments used as probe KTS1(2.4kb), KTS2(4.3kb) and KTS3(1.5kb) were labeled digoxigenin-11-dUTP for the Southern hybridization. In Southern hybridization, all of the probes(KTS1, KTS2 and KTS3) reacted specifically to T sergenti DNA, but not to bovine leucocyte DNA. In order to find out the sensitivities of the digoxigenin-11-dUTP-labeled KTS1 and KTS3 as the probes, purified merozoite DNA and bovine DNA(control) were checked by dot blot hybridization with the probes. Both of the probes, KTS1 and KTS3, detected as minimum amount of 975pg of the T sergenti DNA, but not bovine DNA even to 500ng.

天然 및 養殖産 뱀장어의 蛋白質 및 아미노酸 組成比較

崔鎭浩,林采喚,崔映準,卞大錫,金昌睦,吳成基 경희대학교 부설 식량자원개발연구소 1986 硏究論文集 Vol.7 No.-

우리나라 전역에 걸쳐서 分布하고 있는 뱀장어의 營養學的 基礎資料를 提示하기 위하여 天然産과 養殖産으로 分類하여 蛋白質의 組成, 蛋白質의 아미노산酸 組成을 比較, 分析하였다. 그리고 天然 및 養殖産 뱀장어 肉蛋白質의 組成을 엄밀히 檢討하기 위해 肉蛋白質中 比較的 많은 量을 차지하는 筋形質蛋白質과 筋原纖維蛋白質을 SDS-PAG電氣泳動 分析하여 subunit의 差를 比較하였다. 또한 사료蛋白質의 아미노酸 組成을 分析함으로서 肉蛋白質의 아미노酸 組成에 미치는 영향도 아울러 比較, 檢討하였다. 天然 및 養殖産 뱀장어의 粗蛋白質 合量은 各各 14.3%와 15.4%이었으며, 粗脂肪은 25.0%와 29.7%로서 養殖産 뱀장어의 脂肪 含量이 天然産에 比하여 높은 편이있다. 肉蛋白質을 構成하는 蛋白質 組成은 天然産의 경우 筋形質蛋白質이 30.78%, 筋原纖維蛋白質이 59.02%, 細胞內殘渣蛋白質이 9.73%, 基質蛋白質이 2.47%이었으며, 養殖産은 筋形質蛋白質이 31.81%, 筋原纖維蛋白質이 58.37%, 細胞內殘渣蛋白質이 8.16%, 基質蛋白質이 1.80%를 차지하여 天然産과 養殖産뱀장어의 肉蛋白質 組成은 비교적 유사함을 알 수 있었다. 筋形質蛋白質과 筋原纖維蛋白質 分劃의 一部에 대하여 SDS-PAG電氣泳動 分析을 실시한 結果, 天然産 뱀장어의 筋形質蛋白質은 16개의 subunit로 構成되어 있었고, 養殖産은 分子量이 36,500, 46,000, 58,500, 75,000, 170,000, 235,000에 해당하는 4개의 subunit가 더 많은 22개의 subunit로 構成되어 있었다. 한편 筋原纖維蛋白質은 天然産이 14개의 subunit이었으며, 養殖産은 分子量 45,000에 해당하는 未知의 subunit가 하나 더 많은 15개로 이루어져 있었다. 肉蛋白質의 蛾眉노산 組成은 天然産이 養殖産에 比하여 glycine含量이 다소 높은 것을 제외하고는 대체로 비슷하였으며, lysine, aspartic acid, glutamic acid, glycine이 전체아미노酸의 45% 가량을 점하고 있었다. 사료蛋白質의 아미노酸 組成은 뱀장어의 필수아미노산 要求量을 만족시킬 만큼 양호한 組成을 보여주었으며, aspartic acid와 glutamic acid를 제외하고는 魚肉의 축적단백질로서 魚肉蛋白質의 아미노 組成과 비례관계를 갖는 것으로 판단되었다. The muscles of wild and cultured eel, Anguilla japonica, were analyzed for the protein composition and amino acid profile. The differences of the subunit distribution for the sarcoplasmic and myofibrillar proteins were discussed with sodium dodecylsulfate(SDS) polyacryamide gel electrophoresis. The muscle protein in wild eel was composed 30.78% of sarcoplasmic, 59.02% of myofibrillar, 9.73% of residual intracellular and 2.47% of stroma fraction. That in cultured eel was composed of 31. 81%, 58.37%, 8.16% and 1.80%, respectively. The sarcoplasmic and myofibrillar proteins were composed of 16 and 14 subunits in wild eel, and 22 and 15 subunts in cultured eel. The sarcoplasmic protein between wild and cultured muscles showed a similar trend in the subunits, except a few subunits such as 36,500, 46,000, 58,500, 75,000, 170,000 and 235,000 daltons in cultured eel. Only the existence of 45,000 dalton subunit was the difference between wild and cultured eel in myofibrillar protein. The distribution patterns of total amino acid in muscles of wild and cultured eel were found to be very similar trend, although glycine content in wild eel was slightly higher than that in cultured one.

Microbial pathogens in ticks, rodents and a shrew in northern Gyeonggi-do near the DMZ, Korea

Joon-Seok Chae,유도현,Smriti Shringi,Terry A. Klein,Heung-Chul Kim,Sung-Tae Chong,In-Yong Lee,Janet Foley 대한수의학회 2008 Journal of Veterinary Science Vol.9 No.3

A total of 1,618 ticks [420 individual (adults) and pooled (larvae and nymphs) samples], 369 rodents (Apodemus agrarius, Rattus norvegicus, Tscherskia triton, Mus musculus, and Myodes regulus), and 34 shrews (Crocidura lasiura) that were collected in northern Gyeonggi-do near the Demilitarized Zone (DMZ) of Korea during 2004-2005, were assayed by PCR for selected zoonotic pathogens. From a total of 420 individual and pooled tick DNA samples, Anaplasma (A.) phagocytophilum (16), A. platys (16), Ehrlichia (E.) chaffeensis (63), Borrelia burgdorferi (16), and Rickettsia spp. (198) were detected using species-specific PCR assays. Out of 403 spleens from rodents and shrews, A. phagocytophilum (20), A. platys (34), E. chaffeensis (127), and Bartonella spp. (24) were detected with species-specific PCR assays. These results suggest that fevers of unknown causes in humans and animals in Korea should be evaluated for infections by these vector-borne microbial pathogens.

Identification and prevalence of Ehrlichia chaffeensis infection in Haemaphysalis longicornis ticks from Korea by PCR, sequencing and phylogenetic analysis based on 16S RNA gene

Joon-Seok Chae,Seung-Ok Lee,Dong-Kyeun Na,Chul-Min Kim,Ying-Hua Li,Yoon-Hee Cho,Jin-Ho Park,John-Hwa Lee,Seong-Kug Eo,Terry A.Klein 대한수의학회 2005 Journal of Veterinary Science Vol.6 No.2

Genomic DNAs extracted from 1,288 Haemaphysalis longicornis ticks collected from grass vegetation and various animals from nine provinces of Korea were subjected to screening by genus-specific (Ehrlichia spp. or Anaplasma spp.) real-time TaqMan PCR and speciesspecific (E. chaffeensis) nested-PCR based on amplification of 16S rRNA gene fragments. In all, 611 (47.4%) ticks tested positive for genus-specific amplification of 116 bp fragment of 16S rRNA of Ehrlichia spp. or Anaplasma spp. Subsequently, 396 bp E. chaffeensis-specific fragment of 16S rRNA was amplified from 4.2% (26/611) tick samples. The comparison of the nucleotide sequence of 16S rRNA gene from one tick (EC-PGHL, GeneBank accession number AY35042) with the sequences of 20 E. chaffeensis strains available in the database showed that EC-PGHL was 100% identical or similar to the Arkansas (AF416764), the Sapulpa (U60476) and the 91HE17 (U23503) strains. The phylogenetic analysis also revealed that the E. chaffeensis EC-PGHL formed a single cluster with the above strains. This is the first study to report molecular detection and phylogenetic analysis of E. chaffeensis from H. longicornis ticks in Korea. The implicit significance of E. chaffeensis infection in H. longicornis ticks in Korea is discussed.

Development and Clinical Application of Equine Stem Cell Therapy Products

Joon seok Chae 대한수의학회 2013 대한수의학회 학술대회발표집 Vol.2013 No.-

Morphological Study of Haemadipsa rjukjuana (Hirudinida: Arhynchobdellida: Haemadipisdae) from Gageo-do in Korea

Joon seok Chae,So hyun Won,Baek jun Kim,Hye won Kim,Jun gu Kang,Tae seo Park,Hong yul Seo,Ye Eun,Bae keun Park 대한수의학회 2012 대한수의학회 학술대회발표집 Vol.2012 No.-

Tick (Acari: Ixodidae)-borne Pathogens in Korea

Joon-Seok Chae,Heung-Chul Kim,Terry A. Klein 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04

Nature conservation in the Republic of Korea (ROK), in addition the effects of global warming has increasingly changed Korea’s weather to a subtropical climate has resulted in increased populations of wild animals in association with their ectoparasites. Increased numbers of animal and bird hosts and warmer climate has resulted in higher populations of members of the Family Ixodidae (hard ticks), in addition to habitat and geographical distributions resulting from reforestation. There are; 6 Genera (Amblyomma, Boophilus, Dermacentor, Haemaphysalis, Ixodes, Rhipcephalus) in the Family Ixodidae, and with 35 species. Studies in the ROK over the past 10 years has resulted in the recognition of 3 Genera of Ixodidae (Haemaphysalis, Ixodes, Amblyomma) and 14 species (H. longicornis, H. flava, H. japonica, H. phasiana, H. ornithophila, H. formosensis, Ixodes nipponensis, I. persulcatus, I. turdus, I. pomerantzevi, I. granulatus, I. vespertilionis, I. simplex, Amblyomma testudinarium). A number of tick-borne pathogens, including severe fever with thrombocytopenia (SFTS) virus, tick-borne encephalitis (TBE) virus, Borrelia, Anaplasma, Ehrlichia, Rickettsia, Bartonella, Theileria and Babesia species, have been detected in ticks collected from the ROK. In addition to detecting these pathogens in zoonotic hosts, they have also been detected from pets, domestic animals, and humans. These pathogens often demonstrate vector and host-specificity, while affecting veterinary and medical health. The analysis of vector and pathogen distributions is crucial for the development of diseased mitigation strategies, requiring additional studies to determine the distributions of animal and bird hosts and vectors and associated pathogens.

Identical small subunit ribosomal RNA gene nucleotide sequence of bovine Theileria isolates (Korea and Japan) and Theileria buffeli (Marula, Kenya)

Joon-seok CHAE(채준석),Oh-deog KWON(권오덕),Patricia J. HOLMAN,Suryakant D. WAGHELA,Gerald G. WAGNER,Joo-mook LEE(이주묵) 대한기생충학열대의학회 1998 The Korean Journal of Parasitology Vol.36 No.1