http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Joomin Park (검색결과 5 건)
- 무료
- 기관 내 무료
- 유료
-
Proteome profile changes in SH‐SY5y neuronal cells after treatment with neurotrophic factors
Park, Seyeon,Lee, Joomin Wiley Subscription Services, Inc., A Wiley Company 2011 Journal of cellular biochemistry Vol.112 No.12
<P><B>Abstract</B></P><P>Artemin, one of glial cell line‐derived neurotrophic factors (GDNFs) supports sensory neuron. Although a role of artemin and GDNF as neurite outgrowth regulators in early neuron development has been suggested, the immediate effects of artemin and GDNF on neuronal cells have not been elucidated. Here, we investigated artemin and GDNF actions on the neuronal cell proteome. To identify immediate‐early protein changes by artemin and GDNF in neuronal cells, we used a differential proteomics approach in SH‐SY5y human neuronal cells treated with artemin or GDNF for 1 h. Eleven proteins that changed after both artemin and GDNF treatment were identified using two‐dimensional gel electrophoresis and matrix‐assisted laser desorption ionization time‐of‐flight tandem mass spectroscopy. The calcium ion‐binding chaperone calreticulin and calcium/calmodulin‐binding nuclear matrix protein matrin 3 showed common quantitative differences after both artemin and GDNF treatment. Cytoskeletal proteins also showed quantitative profile differences, which are functionally relevant to cytoskeletal rearrangement leading to the neurite elongation in neurons. These protein changes were detected in neuronal cells without accompanying changes in mRNA levels. These results suggest that immediate changes induced by artemin and GDNF are related to cytoskeletal protein level changes without transcriptional regulation. J. Cell. Biochem. 112: 3845–3855, 2011. © 2011 Wiley Periodicals, Inc.</P>
-
Proteomic analysis to identify early molecular targets of pregabalin in C6 glial cells.
Park, Seyeon,Lee, Joomin Published for the International Federation for Cel 2010 Cell biology international Vol.34 No.1
<P>Pregabalin is a lipophilic amino acid derivative of gamma-amino butyric acid that displays anticonvulsant and analgesic activities against neuropathic pain. Although a role for glial cells as an important player in pain control and also as a new target for pain medicine has been suggested, the effect of pregabalin on glial cells has not been elucidated. In the present study, we have investigated the action of pregabalin on the glial cell proteome. To identify immediate early protein targets of pregabalin in glial cells, a differential proteomics approach in C6 rat glioma cells treated with pregabalin was used. Seven proteins that sensitively reacted to pregabalin treatment were identified using two-dimensional gel electrophoresis and MALDI-TOF-MS (matrix-assisted laser-desorption ionization-time-of-flight MS). The calcium-ion-binding chaperone, calreticulin, and the oxidative response protein, DJ-1, were up-regulated after pregabalin treatment. Hsp (heat-shock protein)-90-beta, cytoskeleton protein actin and myosin also showed quantitative expression profile differences. Functionally relevant to the proteome result, immediate actin depolymerization was observed after treatment with pregabalin. These results suggest a previously undefined role of pregabalin in the regulation of chaperone activity and cytoskeleton remodelling in glial cells.</P>
-
Properties of Heparinoids Premixed with Tumor-Derived Extracellular Vesicles
Manandhar, Sumeet,Park, Jooho,Kothandan, Vinoth K.,Lee, Joomin,Alam, Farzana,Jee, Jun-Pil,Hwang, Jinsu,Byun, Youngro,Hwang, Seung Rim American Chemical Society 2018 Bioconjugate chemistry Vol.29 No.11
<P>Tumor-derived exosomes are bound and internalized to organ-specific cells, affecting metastasis. Heparan sulfate proteoglycans mediate the interaction between cells and exosomes. Exosome transfer to the recipient cell can be competitively blocked by heparinoids, because heparin is structurally similar to heparan sulfate. It is hypothesized that there may be structural requirements of heparinoids to attenuate the cellular uptake and metastatic activity of tumor-derived exosomes. Here, we compared the properties of unfractionated heparin (UFH), glycol-split UFH, low-molecular-weight heparin (LMWH), glycol-split LMWH, and ultra-LMWH premixed with A549-derived exosomes. Uptake of A549-derived exosomes (0.1 mg/mL) into BEAS-2B cells was significantly blocked by 0.4 mg/mL of heparinoids. Heparinoids attenuated migration of BEAS-2B cells stimulated by A549-derived exosomes. Glycol-split LMWH with no antifactor Xa activity exhibited the strongest antimigratory effects than other heparinoids. Thus, heparinoids with proper molecular weight and structure can inhibit tumor-derived exosomes, not proportionally to the anticoagulant activity.</P> [FIG OMISSION]</BR>
-
Jang, Myoung Jin,You, Dalsan,Park, Jin Young,Kim, Kyung,Aum, Joomin,Lee, Chunwoo,Song, Geehyun,Shin, Ha Chul,Suh, Nayoung,Kim, Yong Man,Kim, Choung-Soo Korean Society for Stem Cell Research 2018 International journal of stem cells Vol.11 No.2
<P>Although previous and ongoing clinical studies have used stromal cells during renal ischemia-reperfusion injury (IRI), there is little consensus regarding the optimal protocol. We aimed to optimize the protocol for hypoxic preconditioned human bone marrow-derived mesenchymal stromal cell (HP-hBMSC) therapy in a rat model of renal IRI. We determined the optimal injection route (renal arterial, renal parenchymal, and tail venous injection), dose (low-dose: 1×10<SUP>6</SUP>, moderate-dose: 2×10<SUP>6</SUP>, and high-dose: 4×10<SUP>6</SUP>), and injection period (pre-, concurrent-, and post-IRI). During optimal injection route study, renal arterial injections significantly reduced the decreasing glomerular filtration rate (GFR), as compared to GFRs for the IRI control group, 2 and 4 days after IRI. Therapeutic effects and histological recoveries were the greatest in the group receiving renal arterial injections. During the dose finding study, high-dose injections significantly reduced the decreasing GFR, as compared to GFRs for the IRI control group, 3 days after IRI. Therapeutic effects and histological recoveries were the greatest in the high-dose injection group. While determining the optimal injection timing study, concurrent-IRI injection reduced elevated serum creatinine levels, as compared to those of the IRI control group, 1 day after IRI. Pre-IRI injection significantly reduced the decreasing GFR, as compared with GFRs for the IRI control group, 1 day after IRI. Therapeutic effects and histological recoveries were the greatest in the concurrent-IRI group. In conclusion, the concurrent-IRI administration of a high dose of HP-hBMSC via the renal artery leads to an optimal recovery of renal function after renal IRI.</P>
-
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
