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Short Communications : Efficacy of two virulence gene-deleted Salmonella
( John Hwa Lee ),( Yeong Hwan Choi ),( Min Su Choi ),( Jin Hur ) 한국예방수의학회 2013 예방수의학회지 Vol.37 No.4
We previously developed a novel attenuated Salmonella Typhimurium (S.Typhimurium) △lon△cpxR vaccine. This study was conducted in order to examine whether this vaccine could effectively protect growing piglets against Salmonella infection. Pregnant sows in group A were primed and boosted with the vaccine, whereas pregnant sows in group B received sterile PBS-sucrose. After farrowing, newborn piglets in groups A and B were challenged with a wild type virulent S. Typhimurium at three weeks of age. During the study, serum IgG titers of piglets in group A were significantly higher than those of piglets in group B (P<0.001). In addition, clinical signs were observed in 5.9% of piglets in group A during the entire experimental period after the challenge, while diarrhea was observed in 81.6% of piglets in group B. These results indicate that vaccination of the pregnant sows resulted in effective protection in piglets against Salmonella infection.
John Hwa Lee,Yeong Hwan Choi,Min Su Choi,Jin Hur 한국예방수의학회 2013 예방수의학회지 Vol.37 No.4
We previously developed a novel attenuated Salmonella Typhimurium (S.Typhimurium) △lon△cpxR vaccine. This study was conducted in order to examine whether this vaccine could effectively protect growing piglets against Salmonella infection. Pregnant sows in group A were primed and boosted with the vaccine, whereas pregnant sows in group B received sterile PBS-sucrose. After farrowing, newborn piglets in groups A and B were challenged with a wild type virulent S. Typhimurium at three weeks of age. During the study, serum IgG titers of piglets in group A were significantly higher than those of piglets in group B (P<0.001). In addition, clinical signs were observed in 5.9% of piglets in group A during the entire experimental period after the challenge, while diarrhea was observed in 81.6% of piglets in group B. These results indicate that vaccination of the pregnant sows resulted in effective protection in piglets against Salmonella infection.
Lee, John Hwa The Korean Association of Immunobiologists 2015 Immune Network Vol.15 No.1
In the present study, we investigated the protection conferred by a live attenuated Salmonella enterica serovar Typhimurium (ST) strain against Salmonella Typhimurium, Salmonella Gallinarum (SG), and Salmonella Enteritidis (SE) infection in layer chickens. Birds were orally primed with the attenuated ST strain at 7 days of age and then boosted at 4 weeks post prime immunization (PPI). Sequential monitoring of plasma IgG and mucosal secretory IgA (sIgA) levels revealed that inoculation with ST induced a significant antibody response to antigens against ST, SE, and SG. Moreover, significant lymphoproliferative responses to the 3 Salmonella serovars were observed in the immunized group. We also investigated protection against virulent ST, SE, and SG strain challenge. Upon virulent SG challenge, the immunized group showed significantly reduced mortality compared to the non-immunized group. The reduced persistence of the virulent ST and SE challenge strains in the liver, spleen, and cecal tissues of the immunized group suggests that immunization with the attenuated ST strain may not only protect against ST infection but can also confer cross protection against SE and SG infection.
Lee, Wi Hyoung,Kwak, Donghoon,Anthony, John E.,Lee, Hwa Sung,Choi, Hyun Ho,Kim, Do Hwan,Lee, Seung Goo,Cho, Kilwon WILEY‐VCH Verlag 2012 Advanced functional materials Vol.22 No.2
<P><B>Abstract</B></P><P>The phase‐separation characteristics of spin‐cast difluorinated‐triethylsilylethynyl anthradithiophene (F‐TESADT)/poly(methyl methacrylate) (PMMA) blends are investigated with the aim of fabricating transistors with a high field‐effect mobility and stability. It is found that the presence of PMMA in the F‐TESADT/PMMA blends prevents dewetting of F‐TESADT from the substrate and provides a platform for F‐TESADT molecules to segregate and crystallize at the air–film interface. By controlling the solvent evaporation rate of the spin‐cast blend solution, it is possible to regulate the phase separation of the two components, which in turn determines the structural development of the F‐TESADT crystals on PMMA. At a low solvent evaporation rate, a bilayer structure consisting of highly ordered F‐TESAT crystals on the top and low‐trap PMMA dielectric on the bottom can be fabricated by a one‐step spin‐casting process. The use of F‐TESADT/PMMA blend films in bottom gate transistors produces much higher field‐effect mobilities and greater stability than homo F‐TESADT films because the phase‐separated interface provides an efficient pathway for charge transport.</P>
( John Hwa Lee ) 대한인수공통전염병학회 2018 창립총회 및 학술대회 초록집 Vol.2018 No.1
Pre-stimulation of toll-like receptors (TLRs) by agonists has been shown to increase protection against influenza virus infection. In this study, we evaluated the protective response generated against influenza A/Puerto Rico/8/1934 (PR8; H1N1) virus by oral and nasal administration of live attenuated Salmonella enterica serovar Typhimurium, JOL911 strain, in mice. Oral and nasal inoculation of JOL911 significantly increased the mRNA copy number of TLR2, TLR4 and TLR5, and downstream type I interferon (IFN) molecules, IFN-α and IFN-β, both in peripheral blood mononuclear cells (PBMCs) and in lung tissue. Similarly, the mRNA copy number of interferon-inducible genes (ISGs), Mx and ISG15, were significantly increased in both the orally and the nasally inoculated mice. Post PR8 virus lethal challenge, the nasal JOL911 and the PBS control group mice showed significant loss of body weight with 70% and 100% mortality, respectively, compared to only 30% mortality in the oral JOL911 group mice. Post sub-lethal challenge, the significant reduction in PR8 virus copy number in lung tissue was observed in oral [on day 4 and 6 post-challenge (dpc)] and nasal (on 4 dpc) than the PBS control group mice. The lethal and sub-lethal challenge showed that the generated stimulated innate resistance (StIR) in JOL911 inoculated mice conferred resistance to acute and initial influenza infection but might not be sufficient to prevent the PR8 virus invasion and replication in the lung. Overall, the present study indicates that oral administration of attenuated S. Typhimurium can pre-stimulate multiple TLR pathways in mice to provide immediate early StIR against a lethal H1N1 virus challenge.