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- 검색키워드
- 저자 : Jinxing Song (검색결과 3 건)
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Liping Song,Xia Li,Feng Zu,Changbin Gao,Bincai Wang,Chufa Lin,Jinxing Tu,Aihua Wang,Guolin Zhou 한국유전학회 2019 Genes & Genomics Vol.41 No.12
Background WS24-3A is a newly bred non-heading Chinese cabbage genic male-sterile line, in which sterility is controlled by a recessive gene, designated as Bra2ms. WS24-3A has been used for hybrid breeding. Objective To reveal the underlying molecular mechanisms responsible for the sterility of WS24-3A. Methods Cytological observation of the process of sterile/fertile anther development was performed to determine the tissue and stage in which sterility occurs. Phenotyping and transcriptomic analyses were performed to identify differentially expressed genes (DEGs) between sterile and fertile flower buds at different stages. Results Cytological analysis revealed no tetrads at stage 7 or at later stages of anther development, and the degradation of callose was delayed. Abnormal meiocytes were surrounded by sustaining callose that degenerated gradually in WS24-3A. Comparative transcript profiling identified 3282 DEGs during three anther developmental stages, namely, pre-meiotic anther, meiotic anther, and anthers with single-celled pollen stage. The difference in DEG percentage between up-regulated and down-regulated at meiotic anther stage was obviously larger than at the other two stages; further, most DEGs are important for male meiosis, callose synthesis and dissolution, and tapetum development. Ten DEGs were found to be involved in anther and pollen development, which were analyzed by quantitative PCR. Conclusion Bra2ms affected gene expression in meiocytes and associated with callose synthesis, degradation and tapetum development. Our results provide clues to elucidate the molecular mechanism of genic male sterility in non-heading Chinese cabbage.
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Wang, Ming,Zheng, Jinxing,Song, Yuntao,Li, Ming,Zeng, Xianhu Korean Nuclear Society 2020 Nuclear Engineering and Technology Vol.52 No.9
For proton pencil beam scanning (PBS) technology, the accuracy of the dose distribution in a patient is sensitive to the properties of the incident beam. However, mechanical deformation of the proton therapy facility may occur, and this could be an important factor affecting the proton dose distribution in patients. In this paper, we investigated the effect of deformation on an SC200 proton facility's beam isocenter properties. First, mechanical deformation of the PBS nozzle, L-shape plate, and gantry were simulated using a Finite Element code, ANSYS. Then, the impact of the mechanical deformation on the beam's isocenter properties was evaluated using empirical formulas. In addition, we considered the simplest case that could affect the properties of the incident beam (i.e. if only the bending magnet (BG3) has an error in its mounting alignment), and the effect of the beam optics offset on the isocenter characteristics was evaluated. The results showed that the deformation of the beam position in the X and Y direction was less than 0.27 mm, which meets the structural design requirements. Compared to the mechanical deformation of the L-shape plate, the deformation of the gantry had more influence on the beam's isocenter properties. When the error in the mounting alignment of the BG3 is equal to or more than 0.3 mm, the beam deformation at the isocenter exceeds the maximum accepted deformation limits. Generally speaking, for the current design of the SC200 scanning beam delivery system, the effects of mechanical deformation meet the maximum accepted beam deformation limits. In order to further study the effect of the incident beam optics on the isocenter properties, a fine-scale Monte Carlo model including factors relating to the PBS nozzle and the BG3 should be developed in future research.
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Linxiao Chen,Wei Xia,Jinxing Song,Mengqi Wu,Zhizhen Xu,Xiangyang Hu,Wenqing Zhang 한국식물학회 2020 Journal of Plant Biology Vol.63 No.4
Due to global warming, high temperature has become the main abiotic stress affecting plant growth worldwide. LysM-containing receptor-like kinase 4 (LYK4) is the receptor for chitin, and ERECTA(ER) is a key factor in plant tolerance to high temperature. In this study, we constructed a chitin-induced chimeric LYK4-ER gene, in which the extracellular region and transmembrane domain of the LYK4 gene are fused with the intracellular region of the ER gene. Colony PCR, RT-PCR and western blot analyses of LYK4-ER transcription in plants, confirmed that the LYK4-ER gene was successfully constructed and transferred into Arabidopsis. The LYK4-ER gene localized to the cytomembrane and cytoplasm in vivo because of the binding properties of the transmembrane domain of the LYK4-ER gene to the cell membrane. The transgenic plants showed a higher germination rate and germination index as well as a shorter mean germination time than the wild-type plants, indicating that the LYK4-ER gene increases the heat tolerance of Arabidopsis. The lower H2O2 content and relative electrolytic leakage of the transgenic plants showed that the status of these plants under heat stress was improved. UPLC-MS/MS was used to analyze the phytohormones content, which suggested that the transgenic plants exhibited improved heat tolerance through jasmonic acid signal transduction pathways.
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