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- 저자 : Jiko, Chimari (검색결과 3 건)
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Structural Basis for Selective Binding of Export Cargoes by Exportin-5
Yamazawa, Ryuji,Jiko, Chimari,Choi, Saehae,Park, Il Yeong,Nakagawa, Atsushi,Yamashita, Eiki,Lee, Soo Jae Elsevier 2018 Structure Vol.26 No.10
<P><B>Summary</B></P> <P>In the nucleus, RanGTP binding to importin dissociates the cargo. On the other hand, RanGTP enables exportin to bind export cargo and form the export complex by each exportin's own cargo selection mechanism. Here, we present two X-ray structures for Exportin-5 (Exp-5) alone and Exp-5:RanGTP intermediate complex. The structure of Exp-5 adopts a ring-shaped closed conformation by C-terminal anchor residues 1,167–1,179, interacting with N-terminal heat repeats 4–9. The closed form of Exp-5 is important for the stability of the cargo-free state. Interaction between Exp-5 and RanGTP induces elimination of intramolecular contacts of the C-terminal anchor. A large movement of N-terminal 1–9th heat repeats and C-terminal 19–20th heat repeats creates an open space for RanGTP accommodation. Exp-5 in Exp-5:RanGTP and Exp-5:RanGTP:pre-miRNA adopts the same conformation. RanGTP binding to Exp-5 creates a selective molecular cage area for accepting its cargoes, such as small double-stranded RNAs, without conformational change in Exp-5:RanGTP.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Exp-5 alone structure showed a closed conformation </LI> <LI> Exp-5s in Exp-5:RanGTP and Exp-5:RanGTP:pre-miRNA adopt the same conformation </LI> <LI> RanGTP binding to Exp-5 creates a selective cage for pre-miRNAs and small ds-RNAs </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>[DISPLAY OMISSION]</P>
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흰가루병균 분생포자 ethanol 추출분획의 대맥엽 형광화세포 유도활성
김기청,사산자효,Kim Ki Chung,Shishiyama Jiko 한국응용곤충학회 1979 한국식물보호학회지 Vol.18 No.4
보리흰가루병(Erysiphe graminis hordei race I)에 의한 대맥엽침입부에 항균성 형광화세포를 유도하는 물질의 추출분획을 얻기 위하여 실험을 실시한 결과 분생포자의 ethanol 추출분획이 형광화세포의 유도활성을 가지고 있음이 밝혀졌다. 이 분획은 비친화성인 Turkey 290품종에나 친화성품종에나 마찬가지로 형광화세포 유도활성을 나타냈으나 그 유도에 요하는 시간은 Turkey 290에서 8시간 이내 Kobingataki에서 16시간이내이었다. The autofluorescent cells in the penetration area of powdery mildewed leaves of barley had been reported. The present experiments were performed in order to obtain the fluorescence-inducing extract fraction from the conidia. The preparations were made by extractions and residue which were extracted from the conidia of Erysiphe graminis hordei race I with water, ethanol, and ethyl ether. Bioassaying was carried out on the culled-leaf surface of incompatible Turkey 290 and compatible Kobingataki varieties by placing the drops of extract solutions. Fluorescence-elicitor activity was shown only in tile ethanol-extract fraction to both varieties. However, fluorescence-eliciting rate was more rapid on the leaves of incompatible variety Turkey 290 than on tile those of compatible variety Kobingataki; Turkey 290 less than 8 hours, Kobingataki less than 16 hours.
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