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Jeonghun Lee,Kuk Young Na,Jandee Lee,Su Jin Lee,Young Sil An,Joon Kee Yoon,Euy Young Soh 대한외과학회 2013 Annals of Surgical Treatment and Research(ASRT) Vol.84 No.4
Purpose: Regional lymph node (LN) metastases are detected in 57?85% of patients with papillary thyroid carcinoma (PTC) and are associated with increased tumor recurrence. However, the management of lymphatic disease in patients with PTC has been ongoing source of debate. We have prospectively assessed the usefulness and accuracy of sentinel LN (SLN) biopsy for the detection of LN metastases in patients with PTC on preoperative imaging using single photon emission computed tomography/computed tomography (SPECT/CT) and 99mTc phytate. Methods: We prospectively assessed 39 patients with PTC who had risk factors for recurrence or with the necessity of intraoperative LN sampling for suspicious LN metastases on preoperative imaging from August 2010 to March 2011. The patients underwent preoperative lymphoscintigraphy and SPETC/CT and intraoperative SLN biopsy (SLNB). Results: 99mTc lymphoscintigraphy and SPECT/CT localized SLN in 38 patients (97.4%), with the gamma probe identifying 2.15 mean SLNs in the lateral neck of the 39 patients. Skip metastasis was found in one patient, and lateral compartment LN metastasis in 17 (43.5%). The sensitivity, specificity, and accuracy of SLNB for lateral compartment LN metastasis were 88.2%, 100%, and 94.8%, respectively. SLNB was more accurate and useful for lateral than for central compartment LN metastasis. Conclusion: SPECT/CT improved SLN detection and anatomical localization compared with lymphoscintigraphy. SLNB in patients with risk factors for recurrence or the necessity of intraoperative LN sampling for suspected LN metastases on preoperative imaging was accurate in detecting LN metastases and may help in deciding whether to perform lateral compartment dissection in patients with PTC.
Lee, Jeonghun,Kim, Hyunjung,Kim, Seahee,Lee, Hyemi,Kim, Jin,Kim, Namkug,Park, Heon Joo,Choi, Eun Kyoung,Lee, Jin Seong,Kim, Chulhee The Royal Society of Chemistry 2012 Journal of materials chemistry Vol.22 No.28
<P>We report on the dual function of a silica–iron oxide hybrid nanoparticle combined with a stimulus responsive gatekeeper on the surface. Multifunctional hybrid nanoparticles (Fe@Si–DOX–CD–PEG) composed of an Fe<SUB>3</SUB>O<SUB>4</SUB> core, a mesoporous silica shell, and GSH-responsive CD gatekeepers are an excellent platform for anticancer drug delivery and MR imaging. In particular, the CD gatekeepers on the surface of the hybrid nanoparticle play a key role in accommodating anticancer drug molecules in the pore of the silica shell without premature release until CD gatekeepers are cleaved by GSH. We confirmed, from an <I>in vitro</I> study with the A549 cell line, that DOX was released from the internalized carriers due to GSH-mediated cleavage of the CD gatekeeper. Consequently, apoptotic and clonogenic death occurred in the cells treated with Fe@Si–DOX–CD–PEG. The accumulation of Fe@Si–DOX–CD–PEG in the tumors was detected by <I>in vivo</I> MR imaging. The growth of the tumor <I>in vivo</I> was effectively suppressed by the intravenously injected Fe@Si–DOX–CD–PEG. In addition, the results of the <I>in vivo</I> MR imaging reflected the <I>in vivo</I> inhibition of the cancer growth by Fe@Si–DOX–CD–PEG.</P> <P>Graphic Abstract</P><P>Multimodal hybrid nanoparticles composed of a Fe<SUB>3</SUB>O<SUB>4</SUB> core, a mesoporous silica shell, and GSH-responsive CD gatekeepers are an excellent theranostic platform for MR imaging and anticancer drug delivery not only <I>in vitro</I> but also <I>in vivo</I>. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2jm32137h'> </P>
KIM, SANGMIN,KIL, WON HO,LEE, JEONGMIN,OH, SOO-JIN,HAN, JEONGHUN,JEON, MYEONGJIN,JUNG, TAEWOO,LEE, SE KYUNG,BAE, SOO YOUN,LEE, HYUN CHUL,LEE, JUN HO,YI, HA WOO,KIM, SEOK WON,NAM, SEOK JIN,LEE, JEONG E Spandidos Publications 2014 ONCOLOGY REPORTS Vol.32 No.6
<P>Expression of the CD44 gene is upregulated in breast cancer cells and is correlated with patient survival. Aberrant CD44 expression promotes tumor progression and metastasis. In the present study, we investigated the role of zerumbone (ZER) on regulatory mechanisms of CD44 expression in breast cancer cells. Our results showed that CD44 expression was significantly increased by epidermal growth factor receptor (EGFR) ligands in SKBR3 breast cancer cells. In contrast, EGF-induced CD44 expression was decreased by a MEK1/2 inhibitor, UO126, or STAT3 inhibitor, STAT3 VI, respectively. Notably, ZER downregulated the basal level of CD44 expression in CD44+ breast cancer cells. In addition, the induction of CD44 expression by EGFR ligands, EGF or TGF-α, was markedly decreased by ZER treatment. Finally, we investigated the inhibitory mechanism of ZER on EGF-induced CD44 expression. Our results showed that EGF-induced phosphorylation of STAT3 was completely suppressed by ZER. Collectively, ZER suppressed EGF-induced CD44 expression through inhibition of the STAT3 pathway. Therefore, we suggested that ZER may act as a promising therapeutic drug for the treatment of breast cancer.</P>
Lee, Jeonghun,Oh, Eun-Taex,Lee, Jinyoung,Kang, Taehyeong,Kim, Ha Gyeong,Kang, Hansol,Park, Heon Joo,Kim, Chulhee The Royal Society of Chemistry 2019 NEW JOURNAL OF CHEMISTRY Vol.43 No.3
<P>Adopting conformational conversion of a peptide as a trigger to control on-off gatekeeping on the surface of mesoporous silica nanoparticles has several advantages. Here, we designed a dual-functional cyclic peptide with an iRGD sequence as a stimulus-responsive on-off gatekeeper on the surface of mesoporous silica nanocontainers. The cyclic peptide gatekeeper with an intramolecular disulfide bond between the two cysteine units at both terminals of the peptide sequence exhibited selective drug release triggered by stimulus-induced conformational conversion. Furthermore, the iRGD sequence of the cyclic peptide gatekeeper enhanced the intracellular uptake and therapeutic efficacy of the nanoparticles by specifically binding with NRP-1 receptor on the surface of target cancer cells.</P>
Lee, Jeonghun,Kim, Hyunmi,Han, Songyi,Hong, Eunjung,Lee, Keun-Hyeung,Kim, Chulhee American Chemical Society 2014 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.136 No.37
<P>The use of peptides as gatekeepers for payloads of mesoporous silica nanoparticles would allow triggering the release of guests by various biological stimuli. We investigated the effect of peptide conformation on their gatekeeping capability by employing two model peptides with a turn or a random structure. The conformation-dependent gatekeeping properties provided an opportunity to utilize the conformational conversion of peptides as a valuable motif for stimuli-responsive gatekeepers. Based on that investigation, we demonstrated that Fmoc-CGGC-SS-Si, which exhibited a zero-release property without any stimuli due to a turn-like conformation induced by the intramolecular disulfide bond, can be triggered to release guests by converting its conformation to a random structure, induced by reduction of the disulfide bond upon addition of glutathione. We further demonstrated that the conformational conversion of Fmoc-CGGC by Zn(II) ion can also be utilized as a triggering motif.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/2014/jacsat.2014.136.issue-37/ja507767h/production/images/medium/ja-2014-07767h_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ja507767h'>ACS Electronic Supporting Info</A></P>
Lee, Sang-Ryung,Yim, Hyunee,Han, Jae Ho,Lee, Kyi Beom,Lee, Jeonghun,Soh, Euy Young,Kim, Dae Jung,Chung, Yoon-Sok,Jeong, Seon-Yong,Sheen, Seung Soo,Park, So Hyun,Kim, Jang-Hee American Society for Clinical Pathology 2015 American journal of clinical pathology Vol.143 No.3
<P><B>Objectives:</B></P><P>We evaluated the utility of the VE1 antibody that can detect a mutant protein resulting from the <I>BRAF</I> V600E mutation as a diagnostic tool for thyroid fine-needle aspiration cytology (FNAC).</P><P><B>Methods:</B></P><P>We performed VE1 immunocytochemistry on 202 FNAC specimens from surgically confirmed thyroid nodules. The results were compared with the molecular analyses of the <I>BRAF</I> mutation in these specimens matched with their corresponding histology.</P><P><B>Results:</B></P><P>Diagnoses of FNAC specimens included benign (9.4%), atypia of undetermined significance/follicular lesion of undetermined significance (11.4%), follicular neoplasm/suspicious for follicular neoplasm (2.0%), suspicious for malignancy (9.4%), and malignancy (65.8%). VE1 immunostaining was positive in 71.3% of FNAC specimens. The overall sensitivity of the VE1 antibody was 88.8%, specificity was 71.2%, positive predictive value was 88.2%, negative predictive value was 72.4%, and diagnostic accuracy was 83.7%.</P><P><B>Conclusions:</B></P><P>VE1 immunocytochemistry in thyroid FNAC as a screening test for <I>BRAF</I> mutations is highly specific for malignant category cases but can be suboptimal due to its high false-positive rate for the nonmalignant cases.</P>