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Evaluation of Morus alba leaf extracts after bioconversion for development of anti-diabetic agent
Ive Farha MOONMOON,Jin Hyeok KIM,Tae Jung KIM,Kyung Tae KIM,Young Ho KIM,Cheong Won CHO,Hyung Min KIM,Jong Seong KANG 한국분석과학회 2021 학술대회논문집 Vol.2021 No.11
Morus alba has been widely used as herbal medicines and functional foods for its well known bioactivities, such as antitumor, antidiabetic, and antioxidant activities. Recently, many studies have been reported which improved the bioactivities and function of materials using bioconversion technology such as fermentation by microorganisms and enzymes. Therefore, we applied bioconversion method to M. alba to evaluate enhanced bioactivities. The goal of this study was to develop analytical method to evalutate improved bioactivities and to conduct quality control of bio-converted M. alba leaf products. First, liquid-liquid extraction was performed with water and ethyl acetate to achieve best extraction efficiency and to minimize the solvent matrix effects. The water layer was analyzed by HPLC-DAD-ESI-MS on a C18 column (4.6 × 250 mm, 5 ㎛) with 0.1% formic acid in water (A) and 0.1% formic acid in acetonitrile (B). Flow rate was set as 0.7 mL/min with gradient condition, detection with 320 nm of UV detector, and in room temperature. As a result, five peaks were increased upon bioconversion of M. alba leaves. Among increased peaks, four peaks were identified by comparison of UV spectra and molecular weight with the literature and MS spectrum aquired by LC-MS. It was identified as trans-caffeic acid, morin 3-O-ß-D-glucopyranoside, moracin M 3’-O-ß-glucopyranoside, and astragalin. These compounds were well known for bioactivities such as anti-diabetes. Therefore, the increased peaks would help to explain the enhanced bioactivities of enzyme processed M. alba leaves. Collectively, these compounds can be a marker candidates for the quality control of enzyme processed M. alba leaf extracts.
Chong Woon CHO,Farha Ive MOONMOON,Jin Hyeok KIM,Dan GAO,Hyung Min KIM,Jong Seong KANG 한국분석과학회 2021 학술대회논문집 Vol.2021 No.11
Recently, sulfur hexafluoride (SF6) and octafluoropropane (C₃F8) in ophthalmology have been used to treat the retinal detachment by pneumatic retinopexy. However, the fatal medical accidents such as blindness have been reported during the treatment of retinal detachment with these gases. Additionally, these gases as medical gases have no regulation for safety management in each country of the world. The purpose of this study is to develop the analytical method and evaluate the content of main compounds and impurities in these gases for the safety management using GC-BID. GC-BID analysis was carried out on two type columns as follows. One was a capillary column for analyzing carbon dioxide, carbon tetrafluoride, and hexafluoroethane as well as two main compounds. The other was two type of the packed column for analyzing hydrogen, oxygen, nitrogen, methane, and carbon monoxide. The developed method for the impurities and main compounds in sample gases was validated according to ICH guidelines. And, the content of main compounds in two gases were not less than 99.99%. Additionally, the impurities of C₃F8 were hydrogen (< 0.666 ppmv), oxygen (8.2 ppmv) nitrogen (22.7 ppmv), methane (< 0.087 ppmv), carbon monoxide (< 0.126 ppmv) and others (< 20.0 ppmv). While, those of SF6 were oxygen (23.1 ppmv), nitrogen (30.2 ppmv) and others (not more than LODs). The content of all main components and impurities in two gases were suitable in an accordance with inner content criteria. And severe toxic compounds in all two gases were not observed. In conclusion, the content criteria and analytical methods will be useful for the quality control and safety management of impurities and main compound contents in SF6 and C₃F8 samples.