Specific and Sensitive Detection of Venturia nashicola, the Scab Fungus of Asian Pears, by Nested PCR

Koh, Hyun Seok,Sohn, San Ho,Lee, Young Sun,Koh, Young Jin,Song, Jang Hoon,Jung, Jae Sung The Korean Society of Plant Pathology 2013 Plant Pathology Journal Vol.29 No.4

The fungus Venturia nashicola is the causal agent of scab on Asian pears. For the rapid and reliable identification as well as sensitive detection of V. nashicola, a PCR-based technique was developed. DNA fingerprints of three closely related species, V. nashicola, V. pirina, and V. inaequalis, were obtained by random amplified polymorphic DNA (RAPD) analysis. Two RAPD markers specific to V. nashicola were identified by PCR, after which two pairs of sequence characterized amplified region (SCAR) primers were designed from the nucleotide sequences of the markers. The SCAR primer pairs, designated as D12F/D12R and E11F/E11R, amplified 535-bp and 525-bp DNA fragments, respectively, only from genomic DNA of V. nashicola. The specificity of the primer sets was tested on strains representing three species of Venturia and 20 fungal plant pathogens. The nested PCR primer pair specific to V. nashicola was developed based on the sequence of the species-specific 525-bp DNA fragment amplified by primer set E11F/E11R. The internal primer pair Na11F/Na11R amplified a 235-bp fragment from V. nashicola, but not from any other fungal species tested. The nested PCR assay was sensitive enough to detect the specific fragment in 50 fg of V. nashicola DNA.

DCPP1 is the mouse ortholog of human PAUF that possesses functional analogy in pancreatic cancer

Song, H.,Song, J.,Kim, Y.J.,Jeong, H.H.,Min, H.J.,Koh, S.S. Academic Press 2017 Biochemical and biophysical research communication Vol. No.

Pancreatic adenocarcinoma upregulated factor (PAUF) overexpressed in pancreatic ductal adenocarcinoma (PDAC) plays a major role in tumor progression and metastasis by autocrine and paracrine manners. However, underlying molecular mechanism of PAUF functioning in pancreatic cancer are not fully understood yet. The objective of this study was to evaluate the potential of demilune cell and parotid protein 1 (DCPP1) as a putative mouse ortholog of human PAUF by sequence alignment and functional studies. Overexpression of mouse DCPP1 in Chinese hamster ovary (CHO) cells or pancreatic cancer cells increased cell proliferation, migration, invasion, and adhesion ability in vitro. Treatment of human pancreatic cancer cells with recombinant mouse DCPP1 elevated cell growth, motility, invasiveness, and adhesiveness. Mouse DCPP1 exerted its function on pancreatic cancer cells by activating intracellular signaling pathways involved in aggressive cancer phenotype of human pancreatic cancer cells. Moreover, subcutaneous injection of mice with DCPP1-overexpressing CHO cells increased tumor sizes. Taken together, we conclude that mouse DCPP1 is a multifunctional promoter of tumor growth through functional activation of pancreatic cancer cells, suggesting it to be an ortholog of human PAUF.

A Case of Pleural Paragonimiasis Confused with Tuberculous Pleurisy

Song, Junwhi,Hong, Goohyeon,Song, Jae-Uk,Kim, Wooyoul,Han, Seo Goo,Ko, Yousang,Chang, Boksoon,Jeong, Byeong-Ho,Eom, Jung Seop,Lee, Ji Hyun,Jhun, Byung Woo,Jeon, Kyeongman,Kim, Hong Kwan,Koh, Won-Jung The Korean Academy of Tuberculosis and Respiratory 2014 Tuberculosis and Respiratory Diseases Vol.76 No.4

Here, we report a case of pleural paragonimiasis that was confused with tuberculous pleurisy. A 38-year-old man complained of a mild febrile sensation and pleuritic chest pain. Radiologic findings showed right pleural effusion with pleural thickening and subpleural consolidation. Adenosine deaminase (ADA) activity in the pleural effusion was elevated (85.3 IU/L), whereas other examinations for tuberculosis were negative. At this time, the patient started empirical anti-tuberculous treatment. Despite 2 months of treatment, the pleural effusion persisted, and video-assisted thoracoscopic surgery was performed. Finally, the patient was diagnosed with pleural paragonimiasis based on the pathologic findings of chronic granulomatous inflammation containing Paragonimus eggs. This case suggested that pleural paragonimiasis should be considered when pleural effusion and elevated ADA levels are observed.

cDNA SYNTHESIS OF TMV AND INSERTION OF TMV SEQUENCE INTO PLANT GENOMES

Song, Eun-Kyung,Koh, Hyung-Kon,Kim, Kyung-Hoon,Song, Jae-Young,Park, Young-In,Park, Won-Mok,Lee, Se-Yong Gyeongsang National University Chinju, Korea 1992 Plant molecular biology and biotechnology research Vol.1992 No.

The Serine Proteinase Inhibitor OsSerpin Is a Potent Tillering Regulator in Rice

( Song Yion Yeu ),( Bong Soo Park ),( Wan Gyu Sang ),( Yang Do Choi ),( Min Chul Kim ),( Jong Tae Song ),( Nam Chon Paek ),( Hee Jong Koh ),( Hak Soo Seo ) 한국식물학회 2007 Journal of Plant Biology Vol.50 No.5

Tillering in rice (Oryza sativa L.) is an important agronomic trait that enhances grain production. A tiller is a specialized grainbearing branch that is formed on a non-elongated basal internode that grows independently of the mother stem. Transgenic rice over-expressing the transcription factor OsTB1, a homologue of maize TB1 (Teosinte Branched 1), exhibits markedly reduced lateral branching without the propagation of axillary buds being affected. However, the tillering mechanism remains unknown. Therefore, to further understand that mechanism, we applied proteomics methodology to isolate the proteins involved. Using two-dimensional gel electrophoresis and mass spectrometry, our analysis of the basal nodes from two rice cultivars that differ in their numbers of tillers showed that a rice serine proteinase inhibitor, OsSerpin, accumulates in great amounts in high-tillering ``Hwachung`` rice. Northern blot analysis revealed that much more OsSerpin transcript is found in ``Hwachung`` than in relatively low-tillering ``Hanmaeum``, likely because of high levels of transcription. Therefore, our data suggest that OsSerpin content determines the extent of lateral branching.

Mission Strategy/Approach : Central Asia

Koh, Moo Song 장로회신학대학교 세계선교연구원 2001 선교와 신학 Vol.7 No.-

American Protestants After 400 Years : A Presbyterian Perspective by James M. Phillips

Koh, Moo Song 장로회신학대학교 세계선교연구원 2001 선교와 신학 Vol.8 No.-

Correlation between Abdominal Fat Distribution and Abdominal Temperature in Korean Premenopausal Obese Women

Song, Eun-Mo,Kim, Eun-Joo,Kim, Koh-Woon,Cho, Jae-Heung,Song, Mi-Yeon The Society of Korean Medicine 2013 대한한의학회지 Vol.34 No.2

Objectives: Considering that homeothermy is a major component of metabolic rate, body temperature might play a role in the pathophysiology of obesity. This study aimed to determine the relationship between abdominal fat distribution and abdominal temperature in Korean, premenopausal, obese women. Methods: Weight and height were measured in 26 premenopausal, obese women to calculate body mass index (BMI). Obesity was defined as a $BMI{\geq}25kg/m^2$. Waist circumference (WC) was also measured as well as abdominal fat by computed tomography (CT) and abdominal temperature by digital infrared thermographic imaging (DITI). Results: Visceral abdominal fat area was found to have a significant negative correlation with the temperature of Guanyuan (CV4, lower abdomen acupoint). We also found the visceral-subcutaneous fat ratio had a significant negative correlation with the temperature of CV4 and Right Tianshu (RST25, lateral navel acupoint). Only visceral fat and its ratio to subcutaneous fat had a significant correlation with abdominal temperature. Subcutaneous fat area and total fat area were not correlated with abdominal temperature. Conclusions: This study suggests that abdominal visceral fat has a significant negative correlation with abdominal temperature. Further study is needed to uncover the relationship between abdominal fat distribution and temperature regulation in obese individuals and to define the role of body temperature in the pathogenesis of obesity.

Body Height Effect on Brain Volumes in Youth Decreases in Old Age in Koreans

Koh, In-Song Korean Society for Bioinformatics 2011 Interdisciplinary Bio Central (IBC) Vol.3 No.3

The MRI (magnetic resonance imaging) volumetric analysis of the brain was performed in 59 healthy elderly Koreans (aged 62-76 years; 34 male, 25 female) to investigate whether the previously reported significant correlations between body height and brain volumes in the young aged Koreans (20's) still exist in the old aged Koreans (60's and 70's). Unlike previously reported significant correlations in the young aged Koreans, neither the correlation between whole brain volume and body height in male nor the correlation between cerebellar volume and body height in female show any significance in the old aged Koreans. The significant correlation between body height and whole brain volume was still observed when both male and female data were combined (r=0.27, P<0.05), but the correlation coef-ficient and the level of significance markedly decreased from those of previously reported Korean youth data (r=0.67, P<0.01). Simple linear regression analysis shows decrease of explanatory power of height (measured in $r^2$) from 44% in the youth group to 7% in the old age group on the variance of whole brain volume. Multiple linear regression analysis shows that age and sex, rather than height, are major explanatory variables for whole brain volume in the old aged Koreans. The loss of correlations in the aged group is suspected to be mainly due to age related brain volume changes.

조선 후기 민중사상과 鄭鑑錄의 기능

고성훈(Koh, Song-Hoon) 한국역사민속학회 2015 역사민속학 Vol.0 No.47

조선 후기 민중사상의 주류는 정감록 사상이다. 정감록의 내용은 李氏를 대신하여 鄭氏가 도읍을 세운다는 ‘李亡鄭興’의 논리가 핵심이다. 이 논리를 실현시키는 방법으로는 海島에서 鄭眞人이 군사를 이끌고 나와 새 왕조를 세운다는 ‘海島起兵說’이 있다. 또 정감록에는 난리가 났을 때 몸을 보전할 수 있는 곳인 ‘山林’이나 ‘弓弓處’와 같은 곳을 제시하고 있다. 정감록의 ‘이망정흥’ 논리는 단독으로, 또는 生佛이나 무속, 미륵신앙과 함께 할 때도 있지만, ‘해도기병설’과 결부되어 있는 경우가 일반적이다. 조선 후기에는 도망노비나 유민을 비롯한 민인들이 해도로 들어갔다. 해도기병설을 현실과 연결하면, 해도에 들어간 사람을 정감록의 해도 군사로, 이들의 수장을 진인으로 볼 수도 있을 것이다. 정감록 관련 민중운동은 시기별, 왕대별로 특징이 있다. 숙종 때는 생불과 미륵신앙관련 사건이 자주 일어나며, 유언비어가 일반적이다. 영조 때는 괘서 사건이 잇따라 발생하였다. 영조 전 기간 동안 조정에서는 변란 유형의 사건이 일어나면 여지없이 무신란의 餘黨 소행으로 간주하였다. 정조 때는 ‘한글 정감록’이 나돌 만큼 정감록이 대중화되었고, 유언비어나 괘서와 함께 ‘거사 모의’가 변란의 주류였다. 조정에서는 정감록 관련 사건이 일어날 경우에 정감록을 차단하고 근절하고자 하였다. 또 국왕의 敎書 등을 통해 성리학의 지배 논리를 강화하면서 민심을 수습해 갔다. 조정에서는 변란의 주역들을 ‘思亂之心’을 가진 자로 인식하고 ‘위정척사’를 강조하였다. 변란 유형 민중운동의 주역들은 주로 ‘몰락양반’이다. 이들은 민인들과 접촉하면서 정감록 사상을 퍼뜨려 민심을 선동하고 변란을 기도하였다. 또한 ‘사란지심’을 품고 장기간에 걸쳐 반복적으로 변란을 기도하였다. ‘異人’과 같은 신이한 능력의 소유자로 불리기도 하였다. 이들 변란 주역의 擧事 명분으로는 신분제 혁파와 경제적 평등을 내세우기도 하였다. 정치적으로는 ‘反正’을 주장하였다. 또 義兵을 자처하기도 하였다. 조선 후기 정감록 사상은 변란 유형 민중운동을 사상적으로 뒷받침하는 역할을 하였고, 사회 변동의 촉매제 기능을 함으로써 민중의식 성장에 영향을 미쳤다.