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- 검색키워드
- 저자 : Imran M. Mirza (검색결과 3 건)
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Kashif Ali,A.K. Sarfraz,Imran M. Mirza,A. Bahadur,S. Iqbal,A. ul Haq 한국물리학회 2015 Current Applied Physics Vol.15 No.8
Maghemite (γ-Fe2O3) nanoparticles have been synthesized using a wet chemical route, optimizing the reaction time, PH value and size of the crystallite during synthesis. The Powder X-ray diffraction (XRD) measurements confirmed the presence of an impurity free maghemite phase in our sample with an average crystallite size of 16 nm as calculated from the DebyeeScherrer equation. In physical characterization, the room temperature hysteresis (M-H loop) and blocking temperature (as observed from the M-T plot) revealed that the particles are in the superparamagnetic phase at room temperature. Dielectric behaviour of γ-Fe2O3 with respect to the variation of frequency and temperature was also performed. At room temperatures, we observe a decaying behaviour of both dielectric constant (έ) and tangent looses (tanδ) at smaller frequencies while at higher frequencies both saturate to smaller constant values. In temperature dependent dielectric properties we notice that the dielectric constant (both real and imaginary parts) show an increasing trend with increasing temperatures but an overall slower enhancement at elevated frequencies. The former effect can be attributed to the possible delocalization of impurities at higher temperatures while the latter effect can be explained as an inability of the electric dipole moments to respond at higher frequencies.
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Lupeol supplementation improves the developmental competence of bovine embryos <i>in vitro</i>
Khan, Imran,Chowdhury, M.M.R.,Song, Seok-Hwan,Mesalam, Ayman,Zhang, Shimin,Khan Khalil, Atif Ali,Jung, Eun-Hee,Kim, Ju-Boong,Jafri, Laila,Mirza, Bushra,Kong, Il-Keun Elsevier 2018 Theriogenology Vol.107 No.-
<P><B>Abstract</B></P> <P>Lupeol is a triterpene with various pharmacological properties. This study investigated the effect of lupeol on the <I>in vitro</I> development of bovine embryos. Oocytes <B>(</B>270 per group, 1620 in total<B>)</B> obtained from slaughterhouse-derived ovaries were matured and fertilized <I>in vitro</I> and then cultured for 8 days in a humidified atmosphere of 5% CO<SUB>2</SUB> in air at 38.5 °C. The <I>in vitro</I> maturation medium was supplemented with 0.5, 1.0, 2.0, 3.0, and 4.0 μM lupeol. Treatment with 2.0 μM lupeol significantly (<I>P</I> < 0.05) improved blastocyst development. Hoechst 33342 staining and terminal deoxynucleotidyl transferase dUTP nick-end labeling showed that treatment with 2.0 μM lupeol improved blastocyst quality by increasing the total cell number and reducing the apoptotic cell number. Confocal microscopy confirmed that treatment with 2.0 μM lupeol significantly (<I>P</I> < 0.05) reduced the level of 8-oxoguanine, an indicator of reactive oxygen species. Lupeol treatment also significantly attenuated protein expression of nuclear factor-kappa B subunit 1 (NFKB1), cyclooxygenase (COX) 2, and CASP3. Real-time PCR analysis of <I>nitric oxide synthase 2</I>, <I>NFKB1</I>, <I>COX2</I>, <I>CASP3</I>, and <I>BCL2-associated X protein</I> supported the immunofluorescence data. In conclusion, lupeol is a potent antioxidant that improves bovine embryo development <I>in vitro</I>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Lupeol is a potent antioxidant and it significantly improves the cleavage and blastocyst developmental potentials <I>in vitro</I>. </LI> <LI> Lupeol protects oocytes against oxidative stress in in <I>vitro</I>-culture system. </LI> <LI> Lupeol improves the embryo quality by increasing the total cell numbers and decreasing the apoptotic nuclei of embryo. </LI> <LI> Lupeol down regulates the NF-kB pathway and improves the bovine embryo quality <I>in vitro</I>. </LI> </UL> </P>
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Khan, Imran,Lee, Kyeong-Lim,Xu, Lianguang,Mesalam, Ayman,Chowdhury, M M R,Joo, Myeong-Don,Mirza, Bushra,Kong, Il-Keun Bioscientifica 2017 Reproduction Vol.153 No.4
<P>Heat stress has large effects on reproduction including conception rate in cattle. In this study, we examined the effects of coagulansin-A (coa-A), a steroidal lactone, on acquired thermo tolerance during <I>in vitro</I> production of bovine embryos. Oocytes were incubated in <I>in vitro</I> maturation (IVM) media with or without coa-A at two different temperatures, 40.5˚C and 42˚C, for 20 h. The treatment of coa-A significantly improved blastocyst development only at 40.5˚C (<I>P</I> < 0.05). Interestingly, immunofluorescence analysis demonstrated that coa-A induced heat shock protein 70 (HSP70) and phosphatidylinositol-3-kinase (PI3K), but significantly attenuated nuclear factor kappa B (NF-κB) and cyclooxygenase-2 (COX2). To determine the expression patterns of related genes at the transcription level, qRT-PCR was performed. Expression of <I>HSP70</I> and <I>PI3K</I> was elevated, whereas expression of <I>NF-κB</I>, <I>COX2</I> and inducible nitric oxide synthase (<I>iNOS</I>) was significantly (<I>P</I> < 0.05) downregulated in the coa-A-treated group compared with the control group. Moreover, pro-apoptotic genes were downregulated, and antiapoptic genes were upregulated in the coa-A group. We also counted the total cell number and apoptotic nuclei at the blastocyst and found that more cell numbers (143.1 ± 1.5) and less apoptotic damages (6.4 ± 0.5) in the coa-A treatment group comparing to control group (131.4 ± 2.0 and 10.8 ± 0.5), indicating the enhanced embryo quality. In conclusion, our results demonstrate that the coa-A not only improved the blastocyst development <I>in vitro</I> but also increased their resistance to heat stress condition through induction of <I>HSP70/PI3K</I>.</P>
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