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RISS 인기검색어
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- 저자 : Iman Rusmana (검색결과 5 건)
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Fermentation Characteristics and Microbial Diversity of Tropical Grass-legumes Silages
Ridwan, Roni,Rusmana, Iman,Widyastuti, Yantyati,Wiryawan, Komang G.,Prasetya, Bambang,Sakamoto, Mitsuo,Ohkuma, Moriya Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.4
Calliandra calothyrsus preserved in silage is an alternative method for improving the crude protein content of feeds for sustainable ruminant production. The aim of this research was to evaluate the quality of silage which contained different levels of C. calothyrsus by examining the fermentation characteristics and microbial diversity. Silage was made in a completely randomized design consisting of five treatments with three replications i.e.: R0, Pennisetum purpureum 100%; R1, P. purpureum 75%+C. calothyrsus 25%;, R2, P. purpureum 50%+C. calothyrsus 50%; R3, P. purpureum 25%+C. calothyrsus 75%; and R4, C. calothyrsus 100%. All silages were prepared using plastic jar silos (600 g) and incubated at room temperature for 30 days. Silages were analyzed for fermentation characteristics and microbial diversity. Increased levels of C. calothyrsus in silage had a significant effect (p<0.01) on the fermentation characteristics. The microbial diversity index decreased and activity was inhibited with increasing levels of C. calothyrsus. The microbial community indicated that there was a population of Lactobacillus plantarum, L. casei, L. brevis, Lactococcus lactis, Chryseobacterium sp., and uncultured bacteria. The result confirmed that silage with a combination of grass and C. calothyrsus had good fermentation characteristics and microbial communities were dominated by L. plantarum.
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Fermentation Characteristics and Microbial Diversity of Tropical Grass-legumes Silages
Roni Ridwan,Iman Rusmana,Yantyati Widyastuti,Komang G. Wiryawan,Bambang Prasetya,Mitsuo Sakamoto,Moriya Ohkuma 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.4
Calliandra calothyrsus preserved in silage is an alternative method for improving the crude protein content of feeds for sustainable ruminant production. The aim of this research was to evaluate the quality of silage which contained different levels of C. calothyrsus by examining the fermentation characteristics and microbial diversity. Silage was made in a completely randomized design consisting of five treatments with three replications i.e.: R0, Pennisetum purpureum 100%; R1, P. purpureum 75%+C. calothyrsus 25%;, R2, P. purpureum 50%+C. calothyrsus 50%; R3, P. purpureum 25%+C. calothyrsus 75%; and R4, C. calothyrsus 100%. All silages were prepared using plastic jar silos (600 g) and incubated at room temperature for 30 days. Silages were analyzed for fermentation characteristics and microbial diversity. Increased levels of C. calothyrsus in silage had a significant effect (p<0.01) on the fermentation characteristics. The microbial diversity index decreased and activity was inhibited with increasing levels of C. calothyrsus. The microbial community indicated that there was a population of Lactobacillus plantarum, L. casei, L. brevis, Lactococcus lactis, Chryseobacterium sp., and uncultured bacteria. The result confirmed that silage with a combination of grass and C. calothyrsus had good fermentation characteristics and microbial communities were dominated by L. plantarum.
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( Yuni Puji Hastuti ),( Iman Rusmana ),( Kukuh Nirmala ),( Ridwan Affandi ),( Yuli Siti Fatma ) 한국미생물 · 생명공학회 2021 한국미생물·생명공학회지 Vol.49 No.3
Microbial community plays important roles in the culture environment of mud crab Scylla serrata. One of the environmental management efforts for the cultivation of S.serrata is by stabilizing microorganisms involved in nitrogen cycle process. The availability of dissolved inorganic nitrogen in its culture environment under a recirculating system closely relates to the nitrogen cycle, which involves both anaerobic and aerobic bacterial activities. Anaerobically, there are two major nitrogen compound degradation processes, i.e., denitrification and dissimilatory nitrate reduction to ammonium (DNRA). This study aimed to identify denitrifying and DNRA bacteria isolated from the recirculating cultivation of S. serrata. The water samples were collected from anaerobic filters called close filter system, which is anaerobically conditioned with the addition of varying physical filter materials in the recirculating mud crab cultures. The results showed that three denitrifying bacterial isolates and seven DNRA bacterial isolates were successfully identified. The phylogenetic analysis based on 16S rRNA gene of the denitrifying bacteria revealed that HIB_7a had the closest similarity to Stenotrophomonas daejeonensis strain MJ03. Meanwhile, DNRA bacterial isolate of HIB_92 showed a 100% similarity to Bacillus sonorensis strain N3, Bacillus vallismortis strain VITS-17, Bacillus tequlensis strain TY5, Geobacillus sp. strain DB24, Bacillus subtilis strain A1, and Bacillus mojavensis strain SSRAI21. This study provides basic information denitrifying and DNRA bacterial isolates identity which might have the potential to be applied as probiotics in aquaculture systems in order to maintain optimal environmental conditions.
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Dominant Enterobacteriaceae in tempeh were primarily originated from soybean
Horizon M. Ilham,Michael Wijaya,Antonius Suwanto,Iman Rusmana 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.6
During tempeh production, boiling was consideredas heat treatment that could significantly reduce oreliminate bacterial population in soybean before fungalinoculation. The objective of this study was to enumerateand trace Enterobacteriaceae communities in pre-boilingsoybean, post-boiling soybean, and fresh tempeh designatedas RTI and EMP. Standard plate count and qRT-PCRwere employed to determine the culturable and non-culturablebacteria, while Enterobacterial Repetitive IntragenicConsensus PCR was conducted to determine theintraspecies genomic variations. Fresh tempeh from bothRTI and EMP contained approximately 107 and 108 CFU/gof Enterobacteriaceae respectively. The number of bacteriain pre-boiling soybean were 10,000 times lower than infresh tempeh. Our study showed that most Enterobacteriaceaewere severely injured or quiescent during boilingprocess and quickly recovered up to 109 CFU/g in freshtempeh. Some Klebsiella isolates found in tempeh weregenetically identical to isolates in soybean, but differentfrom those of medical isolates. This study suggested thatsoybean could be the main origin of Klebsiella in freshtempeh.
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Impact of Tempeh Supplementation on Mucosal Immunoglobulin A in Sprague-Dawley Rats
Susan Soka,AntoniusSuwanto,Dondin Sajuthi,Iman Rusmana 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.4
Tempeh, a well-known Indonesian fermented food made from soybeans, results from mixed-culture fermentation using a diverse group of microorganisms. The presence of many nonviable microorganisms in cooked tempeh may trigger responses in the immune system. Thirty female Sprague-Dawley rats were fed a standard diet supplemented with either non-fermented soybeans or tempeh (uncooked or cooked), for 28 days. Gene expression of intestinal immunoglobulin A (IgA) was analyzed using semi-quantitative real-time PCR, and intestinal IgA was further quantified from the ileum wash using ELISA. There was no significant (p>0.05) difference in IgA gene expression between animals groups receiving feed supplemented with cooked or uncooked tempeh. However, a significant (p<0.05) difference was observed between animals receiving feed supplemented with tempeh and with non-fermented soybeans. Microbial cells in tempeh might increase IgA protein secretion.
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