Computer-Aided Cooling Curve Analysis of A356 Aluminum Alloy

Ihsan-ul-haq,Shin, Je-Sik,Lee, Zin-Hyoung 대한금속재료학회 2004 METALS AND MATERIALS International Vol.10 No.1

Computer aided cooling curve analysis (CA-CCA) is very useful in the foundry industry for easy and fast evaluation of a variety of properties. Typical applications include the prediction of the temperatures and amounts of different phases appearing during solidification and monitoring of the quality of melt in terms of Si- modification, grain refinement, inoculation, and graphite spheoridization. The use of cooling curve analysis can be extended to many other areas of solidification also, assuming the calculated values are reasonably accurate. The calculation of zero curve, which is vital in cooling curve analysis, offers many problems however. In this study, an attempt was made to investigate the problems of zero curve calculation and a new method is suggested to minimize calculation errors. An in-house developed computer program was used for a complete analysis of aluminum alloy A356 to determine the latent heat and solid fraction values.

Artificial rearing of the olive fruit fly Bactrocera oleae (Rossi) (Diptera: Tephritidae) for use in the Sterile Insect Technique: improvements of the egg collection system

( Sohel Ahmad ),( Ihsan Ul Haq ),( Polychronis Rempoulakis ),( Dina Orozco ),( Andrew Jessup ),( Carlos Caceres ),( Hannes Paulus ),( Marc J. B. Vreysen ) 한국잠사학회 2016 International Journal of Industrial Entomology Vol.33 No.1

One major constraint in the development and implementation of a successful and cost-effective area-wide integrated pest management (AW-IPM) programme with a SIT component for Bactrocera oleae (Diptera: Tephritidae) is the ability to produce a large number of high quality mass-reared individuals. The aim of this study was to develop a more efficient and practical egg collection system in an attempt to improve the mass-rearing of this species. The following basic parameters were examined: egg production per female, egg hatch, pupal recovery, pupal weight, adult emergence and percentage of fliers. Three different strains (Israel wild-type, France wild-type, and Greece laboratory) were tested and each strain was evaluated for six generations. Female flies of the Israel strain produced significantly more eggs per female than the other two strains, but egg hatch was significantly lower. Egg hatch of the France wild type and the Greece laboratory strain was similar. For all other parameters, there was no significant difference between strains; however, there was a significant generational effect for all parameters observed. As a result of this study, a protocol was developed for the mass-rearing of this species that included the use of large adult holding cages that could house up to 96,000 flies per cage. The newly developed method of egg collection using a flat wax panel as one of the sides of an adult holding cage proved to be cost-effective, efficient, making colony growth easier for industrial mass-rearing.

Artificial rearing of the olive fruit fly Bactrocera oleae (Rossi) (Diptera: Tephritidae) for use in the Sterile Insect Technique: improvements of the egg collection system

Ahmad, Sohel,Haq, Ihsan ul,Rempoulakis, Polychronis,Orozco, Dina,Jessup, Andrew,Caceres, Carlos,Paulus, Hannes,Vreysen, Marc J.B. Korean Society of Sericultural Science 2016 International Journal of Industrial Entomology Vol.33 No.1

One major constraint in the development and implementation of a successful and cost-effective area-wide integrated pest management (AW-IPM) programme with a SIT component for Bactrocera oleae (Diptera: Tephritidae) is the ability to produce a large number of high quality mass-reared individuals. The aim of this study was to develop a more efficient and practical egg collection system in an attempt to improve the mass-rearing of this species. The following basic parameters were examined: egg production per female, egg hatch, pupal recovery, pupal weight, adult emergence and percentage of fliers. Three different strains (Israel wild-type, France wild-type, and Greece laboratory) were tested and each strain was evaluated for six generations. Female flies of the Israel strain produced significantly more eggs per female than the other two strains, but egg hatch was significantly lower. Egg hatch of the France wild type and the Greece laboratory strain was similar. For all other parameters, there was no significant difference between strains; however, there was a significant generational effect for all parameters observed. As a result of this study, a protocol was developed for the mass-rearing of this species that included the use of large adult holding cages that could house up to 96,000 flies per cage. The newly developed method of egg collection using a flat wax panel as one of the sides of an adult holding cage proved to be cost-effective, efficient, making colony growth easier for industrial mass-rearing.

Synthesis of zinc oxide and silver nanoparticles using ficus palmata - Forssk leaf extracts and assessment of antibacterial activity

Sabaoon Shamshad,Jamshaid Rashid,Ihsan-ul-haq,Naseem Iqbal,Saif Ullah Awan 대한환경공학회 2021 Environmental Engineering Research Vol.26 No.6

Multidrug resistance of bacteria is an emerging human health hazard and warrants development of novel antibacterial agents with more effective mode of action. Here, zinc oxide and silver nanomaterials were prepared using Ficus palmata Forssk leaf extract with efficient antibacterial activity. SEM coupled with EDS confirmed the spherical symmetry with average particle diameter 50 to 65 nm while the XRD confirmed crystalline face centered cubic structure of silver and hexagonal crystallize phase of zinc oxide nanoparticles. Antibacterial activity was evaluated for 8 pathogenic bacterial strains including 3 drug resistant pathogenic strains. The nanoparticles showed enhanced growth inhibition for resistant strains in comparison with the broad-spectrum antibiotics i.e. roxithromycin and cefixime. Minimum inhibitory concentration in μg.mL−1 of silver nanoparticles was found to be as low as 33.3 for resistant Streptococcus haemolyticus; 11.1 for Staphylococcus aureus and E Coli; and 3.7 mu;g.mL−1 for resistant Pseudomonas aeruginosa. Similarly, the minimum inhibitory concentration of zinc oxide nanoparticles was found to be 100 mu;g.mL−1 against resistant Streptococcus haemolyticus and Staphylococcus aureus; 11.1 mu;g.mL−1 for resistant Pseudomonas aeruginosa; and 3.7 mu;g.mL−1 against resistant E coli. Ficus palmata Forssk leaf extracts can be explored effectively for synthesizing active antibacterial nanomaterials as a non-toxic and environmentally benign synthesis route.

Synthesis of zinc oxide and silver nanoparticles using ficus palmata - Forssk leaf extracts and assessment of antibacterial activity

Sabaoon Shamshad,Jamshaid Rashid,Ihsan-ul-haq,Naseem Iqbal,Saif Ullah Awan 대한환경공학회 2021 Environmental Engineering Research Vol.26 No.6

Multidrug resistance of bacteria is an emerging human health hazard and warrants development of novel antibacterial agents with more effective mode of action. Here, zinc oxide and silver nanomaterials were prepared using Ficus palmata Forssk leaf extract with efficient antibacterial activity. SEM coupled with EDS confirmed the spherical symmetry with average particle diameter 50 to 65 nm while the XRD confirmed crystalline face centered cubic structure of silver and hexagonal crystallize phase of zinc oxide nanoparticles. Antibacterial activity was evaluated for 8 pathogenic bacterial strains including 3 drug resistant pathogenic strains. The nanoparticles showed enhanced growth inhibition for resistant strains in comparison with the broad-spectrum antibiotics i.e. roxithromycin and cefixime. Minimum inhibitory concentration in μg.mL<SUP>-1</SUP> of silver nanoparticles was found to be as low as 33.3 for resistant Streptococcus haemolyticus; 11.1 for Staphylococcus aureus and E Coli; and 3.7 μg.mL<SUP>-1</SUP> for resistant Pseudomonas aeruginosa. Similarly, the minimum inhibitory concentration of zinc oxide nanoparticles was found to be 100 μg.mL<SUP>-1</SUP> against resistant Streptococcus haemolyticus and Staphylococcus aureus; 11.1 μg.mL<SUP>-1</SUP> for resistant Pseudomonas aeruginosa; and 3.7 μg.mL<SUP>-1</SUP> against resistant E coli. Ficus palmata Forssk leaf extracts can be explored effectively for synthesizing active antibacterial nanomaterials as a non-toxic and environmentally benign synthesis route.

Genetic Variability among Different Populations of Root Knot Nematodes Based on Their Encumbrance Response to Pasteuria Isolates Using PCR-RFLP

Muhammad Kamran,Nazir Javed,Ihsan Ullah,Shahid Nazir,Shakra Jamil,Muhammad Zafar Iqbal,Huma Abbas,Sajid Aleem Khan,Muhammad Ehetisham ul Haq 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.1

A great variable response was observed when PP-3 and PP-J encumbered with 116 populations of root knot nematode (RKN) at two different temperatures (25 ± 2°C and 30 ± 2°C) and concentrations (104 and 105 spores/ml). The PCR reaction amplified intergenic region between cytochrome oxidase subunit II gene (COII) and large subunit of rRNA gene (lrRNA) of the mitochondrial genome of different RKN species. The primer C2F3 and 1108 identified M. incognita with the highest frequency (52.6%) followed by M. javanica (36.8%) and M. arenaria (10.5%). The sizes of PCR products were 1.7 kb for M. incognita and M. javanica populations while populations of M. arenaria produced 1.1 kb fragment. The digestion with Hinf I yielded three different fragment length patterns on 1.5 % agarose gel. From current research it is concluded that intra-Meloidogyne genetic variability exist in RKN populations which have better encumbrance with P. penetrans.

Genetic Variability among Different Populations of Root Knot Nematodes Based on Their Encumbrance Response to Pasteuria Isolates Using PCR-RFLP

Kamran, Muhammad,Javed, Nazir,Ullah, Ihsan,Nazir, Shahid,Jamil, Shakra,Iqbal, Muhammad Zafar,Abbas, Huma,Khan, Sajid Aleem,Haq, Muhammad Ehetisham ul The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.1

A great variable response was observed when PP-3 and PP-J encumbered with 116 populations of root knot nematode (RKN) at two different temperatures ($25{\pm}2^{\circ}C$ and $30{\pm}2^{\circ}C$) and concentrations ($10^4$ and $10^5$ spores/ml). The PCR reaction amplified intergenic region between cytochrome oxidase subunit II gene (COII) and large subunit of rRNA gene (lrRNA) of the mitochondrial genome of different RKN species. The primer C2F3 and 1108 identified M. incognita with the highest frequency (52.6%) followed by M. javanica (36.8%) and M. arenaria (10.5%). The sizes of PCR products were 1.7 kb for M. incognita and M. javanica populations while populations of M. arenaria produced 1.1 kb fragment. The digestion with Hinf I yielded three different fragment length patterns on 1.5 % agarose gel. From current research it is concluded that intra-Meloidogyne genetic variability exist in RKN populations which have better encumbrance with P. penetrans.

Oxidative stress, consequences and ROS mediated cellular signaling in rheumatoid arthritis

Phull, Abdul-Rehman,Nasir, Bakht,Haq, Ihsan ul,Kim, Song Ja Elsevier Pub. Co 2018 Chemico-biological interactions Vol.281 No.-

<P><B>Abstract</B></P> <P>There are numerous extra- and intra-cellular processes involved in the production of reactive oxygen species (ROS). Augmented ROS generation can cause the damage of biomolecules such as proteins, nucleic acid and lipids. ROS act as an intracellular signaling component and is associated with various inflammatory responses, chronic arthropathies, including rheumatoid arthritis (RA). It is well documented that ROS can activate different signaling pathways having a vital importance in the patho-physiology of RA. Hence, understanding of the molecular pathways and their interaction might be advantageous in the development of novel therapeutic approaches for RA.</P>

Analysis of diesel hydrocarbon decomposition using efficient indigenous bacterial isolate: Bacterial growth and biodegradation kinetics

Shazra Khalid,Aneela Iqbal,Asif Javed,Jamshaid Rashid,Ihsan ul Haq,Mohamed Abou El-Fetouh Barakat,Rajeev Kumar 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.11

Industrialization and urbanization have increased the demand for petroleum hydrocarbons; hence the likelihood of contamination of air, soil, and water bodies increases. The survival and biodegradation capabilities of fifteen bacterial isolates were tested in a harsh diesel environment. The bacteria were isolated from soil samples and identified by 16S rRNA gene sequencing. The biodegradation capability of isolates was performed in batch experiments, and diesel degradation analyses were conducted on gas chromatography-mass spectroscopy (GC-MS). The results revealed that only two bacterial isolates (A1 and E5) sufficiently consumed diesel hydrocarbons as a carbon-based energy source. 16S rRNA sequencing identified both isolates as Bacillus genera. An average of 60% of 3% (v/v) diesel was degraded in about 16 hours. Bacillus sp. E5 strain could degrade about 72% and 68% heavier compounds of C24 and C26. The Monod kinetic model for Bacillus sp. E5 utilizing diesel as a substrate showed maximum specific bacterial growth rate (μmax) as 0.1131 hr-1 at 1%, while 0.1287 hr-1 for 3% diesel. Results suggest that the isolated bacterial strain Bacillus sp. E5 has bioremediation potential and can be used as an alternative method for cleaning contaminated petroleum hydrocarbon field sites for sustainable development.

Coagulansin-A has beneficial effects on the development of bovine embryos <i>in vitro</i> via HSP70 induction

Khan, Imran,Lee, Kyeong-Lim,Fakruzzaman, Md.,Song, Seok-Hwan,Ihsan-ul-Haq,Mirza, Bushra,Yan, Chang ,Guo,Kong, Il-Keun Portland Press Ltd. 2016 Bioscience reports Vol.36 No.2

<P>Coagulansin-A (withanolide) is the steroidal lactone obtained from <I>Withania coagulans</I> which belong to Solanaceae family. The present study investigated the effects of coagulansin-A on bovine oocyte maturation and embryo development <I>in vitro</I>. All these oocytes were aspirated from the ovaries obtained from Korean Hanwoo cows at a local abattoir. To determine whether coagulansin-A has beneficial effects on bovine oocyte maturation <I>in vitro</I>, 355 oocytes per group (control and treated) in seven replicates were subjected with different concentrations (1, 2.5, 5, 7.5 and 10 μM) of coagulansin-A. The coagulansin-A was added in the <I>in vitro</I> maturation (IVM) media followed by <I>in vitro</I> fertilization (IVF) and then <I>in vitro</I> culture (IVC). Only treatment with 5 μM coagulansin-A remarkably (<I>P</I><0.05) improved embryos development (Day 8 blastocyst) having 27.30 and 40.01% for control and coagulansin-A treated groups respectively. Treatment with 5 μM coagulansin-A significantly induced activation of heat shock protein 70 (HSP70) (<I>P</I><0.05). Immunofluorescence analysis revealed that 5 μM coagulansin-A treatment also significantly inhibited oxidative stress and inflammation during bovine embryo development <I>in vitro</I> by decreasing 8-oxoguanosine (8-OxoG) (<I>P</I><0.05) and nuclear factor-κB (NF-κB) (<I>P</I><0.05)<I>.</I> The expressions of HSP70 and NF-κB were also conformed through real-time PCR (RT-PCR). Additionally, the terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) assay confirmed that coagulansin-A treatment significantly improved the embryo quality and reduced bovine embryo DNA damage (<I>P</I><0.05). The present study provides new information regarding the mechanisms by which coagulansin-A promotes bovine embryo development <I>in vitro</I>.</P>