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Iftikhar Ali Khan,Khalid Pervaiz Akhtar,Fazal Akbar,Ishtiaq Hassan,Imran Amin,Muhammad Saeed,Shahid Mansoor 한국식물병리학회 2016 Plant Pathology Journal Vol.32 No.1
Cotton leaf curl is devastating disease of cotton characterized by leaf curling, vein darkening and enations. The disease symptoms are induced by DNA satellite known as Cotton leaf curl Multan betasatellite (CLCuMuB), dominant betasatellite in cotton but another betasatellite known as Chili leaf curl betasatellite (ChLCB) is also found associated with the disease. Grafting experiment was performed to determine if host plant resistance is determinant of dominant population of betasatellite in cotton (several distinct strains of CLCuMuB are associated with the disease). Infected scion of Gossypium hirsutum collected from field (the source) was grafted on G. arboreum, a diploid cotton species, resistant to the disease. A healthy scion of G. hirsutum (sink) was grafted at the top of G. arboreum to determine the movement of virus/betasatellite to upper susceptible scion of G. hirsutum. Symptoms of disease appeared in the upper scion and presence of virus/betasatellite in the upper scion was confirmed via molecular techniques, showing that virus/betasatellite was able to move to upper scion through resistant G. arboreum. However, no symptoms appeared on G. arboreum. Betasatelites were cloned and sequenced from lower scion, upper scion and G. arboreum which show that the lower scion contained both CLCuMuB and ChLCB, however only ChLCB was found in G. arboreum. The upper scion contained CLCuMuB with a deletion of 78 nucleotides (nt) in the non-coding region between Arich sequence and βC1 gene and insertion of 27 nt in the middle of βC1 ORF. This study may help in investigating molecular basis of resistance in G. arboreum.
Khan, Iftikhar Ali,Akhtar, Khalid Pervaiz,Akbar, Fazal,Hassan, Ishtiaq,Amin, Imran,Saeed, Muhammad,Mansoor, Shahid The Korean Society of Plant Pathology 2016 Plant Pathology Journal Vol.32 No.1
Cotton leaf curl is devastating disease of cotton characterized by leaf curling, vein darkening and enations. The disease symptoms are induced by DNA satellite known as Cotton leaf curl Multan betasatellite (CLCuMuB), dominant betasatellite in cotton but another betasatellite known as Chili leaf curl betasatellite (ChLCB) is also found associated with the disease. Grafting experiment was performed to determine if host plant resistance is determinant of dominant population of betasatellite in cotton (several distinct strains of CLCuMuB are associated with the disease). Infected scion of Gossypium hirsutum collected from field (the source) was grafted on G. arboreum, a diploid cotton species, resistant to the disease. A healthy scion of G. hirsutum (sink) was grafted at the top of G. arboreum to determine the movement of virus/betasatellite to upper susceptible scion of G. hirsutum. Symptoms of disease appeared in the upper scion and presence of virus/betasatellite in the upper scion was confirmed via molecular techniques, showing that virus/betasatellite was able to move to upper scion through resistant G. arboreum. However, no symptoms appeared on G. arboreum. Betasatelites were cloned and sequenced from lower scion, upper scion and G. arboreum which show that the lower scion contained both CLCuMuB and ChLCB, however only ChLCB was found in G. arboreum. The upper scion contained CLCuMuB with a deletion of 78 nucleotides (nt) in the non-coding region between Arich sequence and ${\beta}C1$ gene and insertion of 27 nt in the middle of ${\beta}C1$ ORF. This study may help in investigating molecular basis of resistance in G. arboreum.
Improved photocatalytic degradation efficiency of methylene blue via MgAl2O4–graphene nanocomposite
Ali Ahmed,Gul Iftikhar Hussain,Khan Muhammad Zarrar,Javaid Farhan 한국세라믹학회 2023 한국세라믹학회지 Vol.60 No.2
In the present work, Magnesium Aluminate (MgAl2O4) nanoparticles and MgAl2O4–graphene nanocomposites with various graphene weight percentages (1, 2, 3, 4, and 5%) were prepared via sol–gel and ultra-sonication methods, respectively. The formation of the spinel phase was confirmed through XRD analysis. Scanning Electron Microscopy was used to study the morphology of the MgAl2O4 nanoparticles and their anchoring on the graphene sheets. The FTIR analysis also confirmed the presence of tetrahedral and octahedral bands at 695 and 526 cm−1, respectively. For magnesium aluminate nanoparticles, the bandgap was calculated to be 5.4 eV, which decreases to 4.9 eV with 5% graphene loading on the MgAl2O4–graphene nanocomposites. For methylene blue dye, the degradation efficiency of the 5% MgAl2O4–graphene nanocomposites was also found to be higher (90%) as compared to the pristine MgAl2O4 nanoparticles (70%). This increase in efficiency depicts their enhanced photocatalytic activity and strongly suggests that the MgAl2O4–graphene nanocomposites could be a good candidate for industrial wastewater remediation.
Rizvi, Syed Zaki Husain,Shah, Fawad Ali,Khan, Namrah,Muhammad, Iftikhar,Ali, Khan Hashim,Ansari, Muhammad Mohsin,Din, Fakhar ud,Qureshi, Omer Salman,Kim, Kyoung-Won,Choe, Yeong-Hwan,Kim, Jin-Ki,Zeb, A Elsevier 2019 International journal of pharmaceutics Vol.560 No.-
<P><B>Abstract</B></P> <P>The objective of current study was to develop solid lipid nanoparticles-loaded with simvastatin (SIM-SLNs) and investigate their <I>in vivo</I> anti-hyperlipidemic activity in poloxamer-induced hyperlipidemia model. Nano-template engineering technique was used to prepare SIM-SLNs with palmityl alcohol as lipid core and a mixture of Tween 40/Span 40/Myrj 52 to stabilize the core. The prepared SIM-SLNs were evaluated for physicochemical parameters including particle diameter, surface charge, morphology, incorporation efficiency, thermal behaviour and crystallinity. <I>In vitro</I> release profile of SIM-SLNs in simulated gastric and intestinal fluids was evaluated by using dialysis bag technique and anti-hyperlipidemic activity was assessed in hyperlipidemia rat model. SIM-SLNs revealed uniform particle size with spherical morphology, zeta potential of −24.9 mV and high incorporation efficiency (∼85%). Thermal behaviour and crystallinity studies demonstrated successful incorporation of SIM in the lipid core and its conversion to amorphous form. SIM-SLNs demonstrated a sustained SIM release from the lipid core of nanoparticles. SIM-SLNs significantly reduced the elevated serum lipids as indicated by ∼3.9 and ∼1.5-times decreased total cholesterol compared to those of untreated control and SIM dispersion treated hyperlipidemic rats. In conclusion, SIM-SLNs showed a great promise for improving the therapeutic outcomes of SIM via its effective oral delivery.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>