The Combination of Naproxen and Citral Reduces Nociception and Gastric Damage in Rats

Mario I. Ortiz,Martha L. Ramírez-Montiel,Martha P. González-García,Héctor A. Ponce-Monter,Gilberto Castañeda-Hernández,Raquel Cariño-Cortés 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.10

It has been shown that the association of non-steroidal anti-inflammatory drugs with plant extracts can increase their antinociceptive activity, allowing the use of lower doses and, thus, limiting side effects. Therefore, the aim of this study was to examine the effects of the interaction between naproxen and citral on nociception and gastric injury in rats. Naproxen, citral, or combinations of naproxen and citral produced an antinociceptive effect. The administration of naproxen produced significant gastric damage, but this effect was not obtained with either citral or the naproxen-citral combination. The ED50 value was estimated for the individual drugs and an isobologram was constructed. The derived theoretical ED50 for the antinociceptive effect (423.8 mg/kg) was not significantly different from the observed experimental value (359.0 mg/kg); hence, the interaction between naproxen and citral mediating the antinociceptive effect is additive. These data suggest that the naproxen-citral combination interacts at the systemic level, produces minor gastric damage, and potentially has therapeutic advantages for the clinical treatment of inflammatory pain.

Photo-catalysis of phenol derivatives with Fe2O3 nanoparticles dispersed on SBA-15

E. Montiel-Palacios,A. K. Medina-Mendoza,A. Sampieri,C. Angeles-Chávez,I. Hernández-Pérez,R. Suarez-Parra 한양대학교 세라믹연구소 2009 Journal of Ceramic Processing Research Vol.10 No.4

Highly ordered hexagonal (p6mm) mesoporous silica SBA-15 was synthesized through a hydrothermal treatment under acidic conditions. Fe2O3/SBA-15 catalysts were prepared by impregnation of SBA-15 media with iron(III) acetylacetonate, iron(III) chloride or iron(II) sulfate solutions. X-ray Diffraction (XRD), X-ray fluorescence (XRF), high angle annular dark field scanning transmission electron microscopy (HAADF-STEM), energy dispersive X-ray (EDX) spectroscopy and nitrogen physisorption characterization were carried out for SBA-15 and Fe2O3/SBA-15 materials. After impregnation and calcination at 823 K, the iron oxide dispersion in SBA-15 was analyzed by STEM and EDX. FeCl3 provides the highest amount of Fe loading in mesoporous SBA-15. The photocatalytic properties of Fe2O3/SBA-15 samples at pH = 3 and pH = 6 were evaluated in catechol and hydroquinone photodecomposition by inducing visible radiation. The conversion of catechol and hydroquinone, at pH = 3 and pH = 6 with iron(III) acetylacetonate and FeCl3 as the precursor of iron oxide nanoparticles, were measured by UV-Vis spectroscopy, chemical oxygen demand (COD) and total organic carbon (TOC) analysis. Highly ordered hexagonal (p6mm) mesoporous silica SBA-15 was synthesized through a hydrothermal treatment under acidic conditions. Fe2O3/SBA-15 catalysts were prepared by impregnation of SBA-15 media with iron(III) acetylacetonate, iron(III) chloride or iron(II) sulfate solutions. X-ray Diffraction (XRD), X-ray fluorescence (XRF), high angle annular dark field scanning transmission electron microscopy (HAADF-STEM), energy dispersive X-ray (EDX) spectroscopy and nitrogen physisorption characterization were carried out for SBA-15 and Fe2O3/SBA-15 materials. After impregnation and calcination at 823 K, the iron oxide dispersion in SBA-15 was analyzed by STEM and EDX. FeCl3 provides the highest amount of Fe loading in mesoporous SBA-15. The photocatalytic properties of Fe2O3/SBA-15 samples at pH = 3 and pH = 6 were evaluated in catechol and hydroquinone photodecomposition by inducing visible radiation. The conversion of catechol and hydroquinone, at pH = 3 and pH = 6 with iron(III) acetylacetonate and FeCl3 as the precursor of iron oxide nanoparticles, were measured by UV-Vis spectroscopy, chemical oxygen demand (COD) and total organic carbon (TOC) analysis.

Potential Control of Foodborne Pathogenic Bacteria by Pediococcus pentosaceus and Lactobacillus graminis Isolated from Fresh Vegetables

C.J. González-Pérez1,I. Vargas-Arispuro,E. Aispuro-Hernández,C.L. Aguilar-Gil,Y.E. Aguirre-Guzmán,A. Castillo,A. Hernández-Mendoza,J.F. Ayala-Zavala,M.A. Martínez-Téllez1 한국미생물·생명공학회 2019 한국미생물·생명공학회지 Vol.47 No.2

The consumption of fresh vegetables has been related to recurrent outbreaks of foodborne diseases (FBD) worldwide. Therefore, the development of effective alternative technologies is necessary to improve the safety of these products. This study aimed to isolate and identify epiphytic lactic acid bacteria (LAB) from fresh fruits and leafy vegetables and characterize their antagonistic capacity due to their ability to produce bacteriocins or antibacterial compounds. For this, 92 LAB isolates from fruits and leafy vegetables were screened for antagonistic activity. Two strains with the highest and broadest antagonistic activities were selected for further characterization; one from cantaloupe melon (strain CM175) and one from cilantro leaves (strain C15). The cell-free supernatants (CFS) of CM175 and C15 were found to exhibit antagonistic activity against FBD-causing pathogens. The CM175 and C15 strains were identified as Pediococcus pentosaceus and Lactobacillus graminis, respectively. Notably, the P. pentosaceus CM175 CFS stopped the growth of Salmonella Typhimurium, Salmonella Saintpaul, Staphylococcus aureus, and Listeria monocytogenes, and delayed Escherichia coli O157:H7 growth. Moreover, L. graminis C15 CFS delayed the growth of all indicator pathogens, but did not completely stop it. Organic acids and bacteriocin-like molecules were determined to be possibly exerting the observed antagonistic activity of the identified LAB strains. Thus, application of the antagonistic compounds produced by Pediococcus pentosaceus and Lactobacillus graminis could be a novel and ecological strategy in developing antimicrobial biopreservatives for the food industry and mitigating FBD by reducing the biological contamination in fruit and vegetable orchards, mainly via their potential in controlling both gram-negative and gram-positive pathogenic bacteria.

Alcohol dehydrogenase 1 participates in the Crabtree effect and connects fermentative and oxidative metabolism in the Zygomycete Mucor circinelloides

Rosa Angélica Rangel-Porras,Sharel P. Díaz-Pérez,Juan Manuel Mendoza-Hernández,Pamela Romo-Rodríguez,Viridiana Alejandre-Castañeda,Marco I Valle-Maldonado,Juan Carlos Torres-Guzmán,Gloria Angélica Gon 한국미생물학회 2019 The journal of microbiology Vol.57 No.7

Mucor circinelloides is a dimorphic Zygomycete fungus that produces ethanol under aerobic conditions in the presence of glucose, which indicates that it is a Crabtree-positive fungus. To determine the physiological role of the alcohol dehydrogenase (ADH) activity elicited under these conditions, we obtained and characterized an allyl alcohol-resistant mutant that was defective in ADH activity, and examined the effect of adh mutation on physiological parameters related to carbon and energy metabolism. Compared to the Adh+ strain R7B, the ADH-defective (Adh-) strain M5 was unable to grow under anaerobic conditions, exhibited a considerable reduction in ethanol production in aerobic cultures when incubated with glucose, had markedly reduced growth capacity in the presence of oxygen when ethanol was the sole carbon source, and exhibited very low levels of NAD+-dependent alcohol dehydrogenase activity in the cytosolic fraction. Further characterization of the M5 strain showed that it contains a 10-bp deletion that interrupts the coding region of the adh1 gene. Complementation with the wild-type allele adh1+ by transformation of M5 remedied all the defects caused by the adh1 mutation. These findings indicate that in M. circinelloides, the product of the adh1 gene mediates the Crabtree effect, and can act as either a fermentative or an oxidative enzyme, depending on the nutritional conditions, thereby participating in the association between fermentative and oxidative metabolism. It was found that the spores of M. circinelloides possess low mRNA levels of the ethanol assimilation genes (adl2 and acs2), which could explain their inability to grow in the alcohol.