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Streptobacillus sp.가 생산하는 pullulanase에 관한 연구
오두환,지희숙,최형택,신원철,유주현 연세대학교 산업기술연구소 1986 논문집 Vol.18 No.2
A bacterium with the highest pullulanase activity was isolated from the soil and identified as Streptobacillus sp. Y-716. The optimum culture condition of Streptobacillus sp. for the production of pullulanase was as follows; 1.2%(w/v)soluble starch, 0.7%(w/v) CH₃COONH₄, 0.05%(w/v) MgSO₄7 H₂O, 0.01%(w/v)FeSO₄7H₂O, at 30℃, for 48 hrs. The pullulanase from the culture broth of Streptobacillus sp. Y-716 was purified about 46 fold by ammonium sulfate precipitation, dialysis. DEAE-cellulose and Sephadex G-200 column chromatography. The purified enzyme was stable between pH 5.0 and pH 7.5. It was inactivated rapidly above 35℃. The Km value and Vmax of the purified enzyme were 0.0137mg/m/ and 0.666μm/min. on pullulan, respectively.
Risk Factors of Postoperative Pancreatic Fistula in Curative Gastric Cancer Surgery
Yu, Hyeong Won,Jung, Do Hyun,Son, Sang-Yong,Lee, Chang Min,Lee, Ju Hee,Ahn, Sang-Hoon,Park, Do Joong,Kim, Hyung-Ho The Korean Gastric Cancer Association 2013 Journal of gastric cancer Vol.13 No.3
Purpose: Postoperative pancreatic fistula is a dreadful complication after gastric cancer surgery. The purpose of this study is to evaluate the actual incidence and risk factors of postoperative pancreatic fistula after curative gastrectomy for gastric cancer. Materials and Methods: A total of 900 patients who underwent gastrectomy for gastric cancer (laparoscopic gastrectomy, 594 patients; open gastrectomy 306 patients) were enrolled between January 2009 and December 2010. Clinical outcomes, including postoperative pancreatic fistula grade based on the International Study Group on Pancreatic Fistula, were investigated. Results: Overall, the postoperative pancreatic fistula rate was 3.3% (30/900) (1.5% in laparoscopic gastrectomy versus 6.9% in open gastrectomy, P<0.001). Patients who underwent D2 lymphadenectomy, total gastrectomy, splenectomy or distal pancreatectomy showed higher postoperative pancreatic fistula rates (4.7%, 13.8%, 13.6%, or 57.1%, respectively, P<0.001). Patients with postoperative pancreatic fistula had higher morbidity (46.7% versus 13.1%, P<0.001), delayed gas out (4.9 days versus 3.8 days, P<0.001), belated diet start (5.8 days versus 3.5 days, P<0.001) and longer postoperative hospital stay (13.7 days versus 6.8 days, P<0.001). On the multivariate analysis, total gastrectomy (odds ratio 9.751, 95% confidence interval: 3.348 to 28.397, P<0.001), distal pancreatectomy (odds ratio 7.637, 95% confidence interval: 1.668 to 34.961, P=0.009) and open gastrectomy (odds ratio 2.934, 95% confidence interval: 1.100 to 7.826, P=0.032) were the independent risk factors of postoperative pancreatic fistula. Conclusions: Laparoscopic gastrectomy had an advantage over open gastrectomy in terms of the lower postoperative pancreatic fistula rate. Total gastrectomy and combined resection, such as distal pancreatectomy, should be performed carefully to minimize postoperative pancreatic fistula in gastric cancer surgery.
Hyeong Won Yu,Jee-Hye Choi,Ja Kyung Lee,김우철,June Young Choi 대한내시경복강경외과학회 2022 Journal of Minimally Invasive Surgery Vol.25 No.2
Lateral neck lymph node dissection (LND) along with total thyroidectomy is the standard treatment for thyroid cancer patients with metastases to the lateral neck lymph nodes. In general, lateral neck LND removes lymph nodes located at levels II to V ipsilateral to the thyroid cancer and preserves the spinal accessory nerve, internal jugular vein, and sternomastoid muscle during surgery. This video article was written to introduce the robotic bilateral axillo-breast approach for lateral neck LND and to describe the surgical method.
Hyeong Won Yu,June Young Choi,Young Suk Park,Hyung Sub Park,YoungRok Choi,Sang-Hoon Ahn,Eunyoung Kang,오흥권,Eun-Kyu Kim,Jai Young Cho,Duck-Woo Kim,Do Joong Park,Yoo-Seok Yoon,Sung Bum Kang,Hyung-Ho Kim 대한외과학회 2019 Annals of Surgical Treatment and Research(ASRT) Vol.96 No.5
Purpose: To evaluate superiority of a night float (NF) system in comparison to a traditional night on-call (NO) system for surgical residents at a single institution in terms of efficacy, safety, and satisfaction. Methods: A NF system was implemented from March to September 2017 and big data analysis from electronic medical records was performed for all patients admitted for surgery or contacted from the emergency room (ER). Parameters including vital signs, mortality, and morbidity rates, as well as promptness of response to ER calls, were compared against a comparable period (March to September 2016) during which a NO system was in effect. A survey was also performed for physicians and nurses who had experienced both systems. Results: A total of 150,000 clinical data were analyzed. Under the NO and NF systems, a total of 3,900 and 3,726 patients were admitted for surgery. Mortality rates were similar but postoperative bleeding was significantly higher in the NO system (0.5% vs. 0.2%, P = 0.031). From the 1,462 and 1,354 patients under the NO and NF systems respectively, that required surgical consultation from the ER, the time to response was significantly shorter in the NF system (54.5 ± 70.7 minutes vs. 66.8 ± 83.8 minutes, P < 0.001). Both physicians (90.4%) and nurses (91.4%) agreed that the NF system was more beneficial. Conclusion: This is the first report of a NF system using big data analysis in Korea, and potential benefits of this new system were observed in both ward and ER patient management
Yu Hyeong Won,Lee Ja Kyung,Kim Woochul,Kwak JungHak,Kim Su-jin,Chai Young Jun,Choi June Young,Lee Kyu Eun 대한내분비외과학회 2022 The Koreran journal of Endocrine Surgery Vol.22 No.3
Purpose: Most thyroid nodules are not associated with symptoms, even if they are thyroid carcinomas. However, the thyroid gland is located superficially in the neck, and is therefore easy to palpate with the appropriate training. We aimed to develop and evaluate the feasibility of a method of self-examination of the thyroid gland for the detection of nodules. Methods: Between August 2014 and February 2015, 554 patients with thyroid nodules visited the outpatient clinic of the Thyroid Cancer Center, Seoul National University Bundang Hospital. The patients were trained to palpate their nodules using a brochure and the results were compared with those of examinations by a physician. Results: The sensitivity and specificity of thyroid self-examination were 66.3% and 81.7%; and the positive and negative predictive values were 80.7% and 67.7%, respectively. The false positive rate was 18.3%. After the patients had been trained, the thyroid nodule detection rate increased from 66.3% to 88.4%. Conclusion: Thyroid self-examination was found to be easy to learn and represents a feasible method of screening for thyroid nodules in an outpatient setting.