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      • KCI등재

        Theoretical Study of the Elastic and the Thermodynamic Properties of Re$_2$C under High Pressure

        Hui-Ru Lei,Li-Hong Zhang,Xu Li,Xiao-Zhen Gao,Jing Li 한국물리학회 2019 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.74 No.11

        The structural properties of Re$_2$C in anti-MoS$_2$ and anti-ReB$_2$ structures have been investigated by using the pseudopotential plane wave methods based on the density functional theory. The anti-ReB$_2$ structure is found to be more stable than the anti-MoS$_2$ structure. In particular, for the first time, we have studied the elastic properties of Re$_2$C in the anti-ReB$_2$ structure under high pressure. The ductile-brittle behavior and Vickers hardness for the anti-ReB$_2$ structure are also been analyzed. In addition, the Debye temperature, heat capacity and thermal expansion coefficient are discussed by using the quasiharmonic Debye model method.

      • KCI등재

        Isolation and characterization of LHT-type plant amino acid transporter gene from Panax ginseng Meyer

        Ru Zhang,Jie Zhu,Hong-Zhe Cao,Xiao-Lei Xie,Jing-Jia Huang,Xiang-Hui Chen,Zhi-Yong Luo 고려인삼학회 2013 Journal of Ginseng Research Vol.37 No.3

        A lysine histidine transporter (LHT) cDNA was isolated and characterized from the roots of Panax ginseng, designated PgLHT. The cDNA is 1,865 bp with an open reading frame that codes for a protein with 449 amino acids and a calculated molecular mass of 50.6 kDa with a predicted isoelectric point of 8.87. Hydropathy analysis shows that PgLHT is an integral membrane protein with 9 putative membrane-spanning domains. Multiple sequence alignments show that PgLHT shares a high homology with other plant LHTs. The expression profile of the gene was investigated by real-time quantitative polymerase chain reaction during various chemical treatments. PgLHT was up-regulated in the presence of abscisic acid, salicylic acid, methyl jasmonate, NaCl, and amino acids. To further explore the function of PgLHT gene, full-length cDNA of PgLHT was introduced into P. ginseng by Agrobacterium rhizogenes A4. The overexpression of PgLHT in the hairy roots led to an obviously increase of biomass compared to the controls, and after addition of the amino acids, the overexpressed-PgLHT hairy roots grew more rapidly than untreated controls during early stage of the culture cycle. The results suggested that the PgLHT isolated from ginseng might have role in the environmental stresses and growth response.

      • SCIESCOPUSKCI등재

        Isolation and characterization of LHT-type plant amino acid transporter gene from Panax ginseng Meyer

        Zhang, Ru,Zhu, Jie,Cao, Hong-Zhe,Xie, Xiao-Lei,Huang, Jing-Jia,Chen, Xiang-Hui,Luo, Zhi-Yong The Korean Society of Ginseng 2013 Journal of Ginseng Research Vol.37 No.3

        A lysine histidine transporter (LHT) cDNA was isolated and characterized from the roots of Panax ginseng, designated PgLHT. The cDNA is 1,865 bp with an open reading frame that codes for a protein with 449 amino acids and a calculated molecular mass of 50.6 kDa with a predicted isoelectric point of 8.87. Hydropathy analysis shows that PgLHT is an integral membrane protein with 9 putative membrane-spanning domains. Multiple sequence alignments show that PgLHT shares a high homology with other plant LHTs. The expression profile of the gene was investigated by real-time quantitative polymerase chain reaction during various chemical treatments. PgLHT was up-regulated in the presence of abscisic acid, salicylic acid, methyl jasmonate, NaCl, and amino acids. To further explore the function of PgLHT gene, full-length cDNA of PgLHT was introduced into P. ginseng by Agrobacterium rhizogenes A4. The overexpression of PgLHT in the hairy roots led to an obviously increase of biomass compared to the controls, and after addition of the amino acids, the overexpressed-PgLHT hairy roots grew more rapidly than untreated controls during early stage of the culture cycle. The results suggested that the PgLHT isolated from ginseng might have role in the environmental stresses and growth response.

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