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벼 葯培養에서 低溫 前處理가 callus 및 植物體 分化率에 미치는 影響
Huhn Pal Moon(文憲八),Sun Hee Choi(崔善嬉),Kyung Ho Kang(姜京鎬),Soo Yeon Cho(趙守衍) 한국육종학회 1989 한국육종학회지 Vol.21 No.3
The increasing callus induction and plant regeneration is the first target to apply anther culture technique to rice varietal improvement. The effects of low-temperature pretreatment on the callus induction and plant regeneration were examined with different temperatures and different treatment durations in four rice varieties. Low-temperature treatment on rice anther prior to anther plating increased remarkably both callus induction and plant regeneration, especially in the callus induction. The treatment temperature was more critical than the duration. In general, the proper treatment would be 8-12℃ in temperature and 8-16 days in duration with higher temperature for Tongil type rice varieties, which were derived from indical/japonica hybridization. Highly significant correlation was found between the callus induction and plant regeneration in Tongil type rice varieties, Milyang 23 and Taebaegbyeo, while no significant correlation in Japonica varieties. The ratio of haploid and diploid plants among the anther-derived plants with lowtemperature pertreatment was varied from different rice varieties, averaging approximately 7 haploids : 3 diploids.
벼葯培養에서 培地 agar 濃度의 變化가 植物體 分化에 미치는 影響
Huhn Pal Moon(文憲八),Sun Hee Choi(崔善姬),Soo Yeon Cho(趙守衍),Young Hee Son(孫永姬) 한국육종학회 1988 한국육종학회지 Vol.20 No.4
The low frequency of plant regeneration is one of the problems in the practical appliction of anther culture technique to rice(Oryza sativa L.) breeding. In addition to the improving chemical composition, the improving physical properties of the culture medium would be necessary in terms of the controlling osmotic potential in the medium for the raising plant regeneration. Rice calli derived from four homozygous varieties and four F₁ combinations in three different callus media were evaluated for plant regeneration with four different concentrations of agar in the regeneration medium. Regardless of plant genotype and callus media, plant regeneration rate was remarkably increased with the increased agar concentration in the plant regeneration medium. The 1.6 and 1.2% agar concentrations gave the highest frequency of plant regeneration. The 1.6% agar medium was also found for the improving callus ability to sub-culture.
벼 葯培養에서 1次培地 植物體分化 硏究 Ⅰ. NAA와 2,4-D 混合 1次培地에 의한 植物體分化
Huhn Pal Moon(文憲八),Sun Hee Choi(崔善姬),Young Hee Son(孫永姬),Soo Yeon Cho(趙守衍),Rae Kyeong Park(朴來敬) 한국육종학회 1988 한국육종학회지 Vol.20 No.3
Anter culture tachnique is practically used for rice varietal development. Rice plantlets via anther culture are usually produced by a two-step procedure: callus induction and plantlet development. An one-step procedure which rice plantlets are generated directly from plated anthers without transferring the calluses to plant regeneration medium would provide the saving labor, space and time in the anter culture. Three media formulations, which cantained the mixtures of NAA(5×10⁻⁶M and 2.4-D(10⁻⁶, 10⁻⁷ and 10⁻⁸M) in the N6 medium, produced rice plantlets directly without transferring calluses to another medium. The plantlets by the one-step procedure were developed about 4 weeks after anthers were placed on the medium. The plant regeneration frequencies with the one-step procedure varied from 0% for “Taebaegbyeo” to 4.8% for “Cheonmabyeo”. In general, the media which contained 5x10⁻⁶M of NAA and 10⁻⁷~ 10⁻⁸M of 2.4-D showed better responses for the one-step procedure of rice anter culture.
Huhn Pal Moon(文憲八),Kyung Ho Kang(姜京鎬)Soo Yeon Cho(趙守衍) 한국육종학회 1991 한국육종학회지 Vol.23 No.2
One-step culture in which the pollen plantlets are produced directly from either embryoids or embryogenic callus from microspores in a single operation of the culture procedure would save labors, time, laboratory materials and spaces required with two-step culture in which callus are induced in a medium and subsequently transferred to the different medium for plant development. Three phytohormones, 2,4-D, kinetin and ABA were added with combined formulations in N6 basal medium to determine the effects on the development of pollen plant through one-step procedure of anther culture without transferring callus. Two japonica(Cheonmabyeo and Chucheongbyeo) and two Tongil-type(indica/japonica) varieties(Milyang 23 and Taebaegbyeo) were used as donor plant materials. All the varieties used produced pollen plants in any one formulation of 24 treatment combinations from 4 weeks after anther plating with the large between- and within-varietal variability:0.6~26.3% for Cheonmabyeo, 0.3~13.5 % for Chucheongbyeo, 0~2.3 % for Milyang 23 and 01.5% for Taebaegbyeo. In general, the N₆ basic medium supplemented with 0.002-0.02mg/ℓ of 2,4-D showed higher plant regenerations for japonica varieties in one-step culture. The combination of 0.002mg/ℓ 2,4-D and 0.5mg/ℓ kinetin enhanced the frequency of direct plant production, while the effect of abscisic acid was not clear.
Heterosis for vegetative stage cold tolerance in rice
Huhn Pal Moon(文憲八),알 엔 카우(R.N. Kaw),Jong Doo Yae(芮鍾斗) 한국육종학회 1987 한국육종학회지 Vol.19 No.4
Heterosis for low-temperature tolerance at the vegetative growth stage was studied in ninety F₁ rice hybrids. The F₁s were transplanted in 2.25-m long single row plots, replicated thrice and were subjected to cold water stress at 17℃ and 20℃, in separate blocks, starting from 20 days after transplanting until maturity. Leaf-discoloration was used as a measure of cold tolerance at the vegetative stage. Average mid-parent heterosis of - 40.7% and high-parent heterosis of - 61.9% was recorded in the japonica/indica group of crosses as compared to -18.6% and -26.0%, respectively, in the indica/indica group. Heterosis for cold hardiness was greater for the japonica/indica group of hybrids with 95.8% hybrids showing significant negative mid-parent heterosis and 100% high-parent heterosis (low leaf-discoloration scores mean high cold hardiness). In indica/indica group, only 6.3% had lower scores in japonica/indica group, suggesting some involvement of complimentary gene action in the inheritance of cold hardiness. Barkat/IR 29506, Shimokita/IR 29506 and Suweon 235 /IR 29506 from the japonica/indica group exhibited better cold tolerance than their respective cold tolerant parents. The correlation between mid-parent value and hybrid perfromance was of sufficient magnitude to indicate that prediction of hybrid performance from parental value should prove effective.
수도 고단백계통 육성을 위한 기초적연구 IV. 단일 및 고온처리가 쌀의 Amylose 및 조단백질 함량에 미치는 영향
허문회,문헌팔,Mun-Hue Heu,Huhn-Pal Moon 한국작물학회 1974 Korean journal of crop science Vol.- No.15
In order to obtain the fundamental informations on the protein and amylose content in rice grain, 4 varieties were subjected to the 4 different shortday treatments in 2 kinds temperature. Results can be summarized as follows; 1). By warmer temperature amylose content in the rice dropped by 2 to 3%, but no significant changes in protein content. 2). Short-day treatment caused decreases in amylose content by 2 to 5% and increases in protein content by 2 to 6%. 3). Interactions among varieties, temperature and shortday was significant for both the amylose content and protein content. 고온과 단일이 쌀의 Amylose 및 단백질함량에 미치는 영향을 알기 위하여 일장감응성정도와 단백질 함량이 다른 4개품종을 고온(온실내)과 상온(실외)에서 전생육기간중 계속단일처리, 출수기까지만 단일처리, 출수기이후만 단일처리하고 무처리자연구와 대조하여 쌀의 Amylose 및 단백질함량을 조사비교하였다. 그 결과는 다음과 같이 요약된다. 1. 고온에 의하여 Amylose함량은 2-3% 낮아졌으나 단백질함량은 큰 변화가 없었다. 2. 단일처리로 Amylose함량을 2-5% 저하시켰고 단백질함량을 2-6%증가시켰다. 전생육기간단일에서 가장 크게 영향됐으며 출수전단일이 출수후단일보다 영향이 컸다. 3. 출수후단일조건은 조생종에서 영향이 컸고 출수전 및 전생육기간단일하에서는 고단백품종보다 저단백품종에서 영향이 컸으며 특히 감광성품종인 진흥은 출수전영향이 컸다. 4. 이상의 결과로 미루어 저Amylose 고단백계통육성을 위해서는 장일저온하의 선발이 효율이 높을 것으로 추론하였다.
Colchicine을 利用한 水稻 葯培養 半數體의 染色體 倍加
Young Hee Son(孫永姬),Huhn Pal Moon(文憲八),Soo Yeon Cho(趙守衍),Rae Kyeong Park(朴來敬) 한국육종학회 1985 한국육종학회지 Vol.17 No.1
The addition of dimethyl sulfoxide(DMSO) and Tween 20 to colchicine solution applied to anther derived rice haploid plants increased the percentage of induced diploids more than did colchicine alone. When Gibberellic acid(GA 3) was added, plant mortality caused by Tween 20 was decreased, thus the percentage of haploids with doubled sectors was increased. Concentration of colchicine solution used was 0.1% and those of DMSO and Tween 20 were 2% and 0.03 %, respectively. At a concentration of 20 ㎎/ℓ, GA3 was more effective.
Estimates of combining ability for cold tolerance at reproductive stage in rice
램 나스 카우(Ram Nath Kaw),Huhn Pal Moon(文憲八),Jong Doo Yae(芮鍾斗),로미 엠 비스페라스(R.M. Visperas) 한국육종학회 1989 한국육종학회지 Vol.21 No.3
Ninety F₁ rice hybrids, produced by crossing 15 rice cultivars having lowtemperature tolerance at different growth stages with 6 high yielding elite IRRI cultivars, were evaluated for combining ability for reproductive stage cold tolerance. Evaluation was done by subjecting the plants to 17℃ and 20℃ water temperatures, in separate blocks, from 20 days after transplanting to maturity. Both general (GCA) and specific (SCA) combining ability variances were significant, with estimates of GCA being larger for all traits, showing predominance of additive type of gene action. The indica parents viz. Samgangbyeo, Suweon 287, Shoa-Nan-Tsan, K 39-96, China 988, IR 9202, Leng Kwang, IR 7167 and IR 15889 proved to be good donors for reproductive stage cold tolerance. Parental performance per se was a good indicator of general combining ability effects of the parents, but no association was observed between per se performance of the crosses and their specific combining ability effects.
Estimates of combining ability for vegetative stage cold tolerance in rice
알 엔 카우(R. N. Kaw),로미 엠 비스페라스(R. M. Visperas),Huhn Pal Moon(文憲八),Jong Doo Yae(芮種斗) 한국육종학회 1986 한국육종학회지 Vol.18 No.1
Ninety F₁ rice hybrids were evaluated for combining abilily for low-temperature tolerance at the vegetative growth stage. Evaluation was done by subjecting the plant to 17℃ and 20℃ water temperatures in separate blocks from 20 days afler transplanting to maturity. Combining ability analysis showed the predominance of additive gene action. The Japonica parents viz. Shimokita, K48, SR5204-91-4-1, Stejaree 45, Barkat and K332 were good donors for vegetative stage cold tolerance. The best sca combinations involved both high and low general combiners. Parental performance per se was a good indicator of gca effects of the parents.