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김종희,홍 민,한준희,이현주,최다혜,Hoang Viet Bach Khoa,Luong Van Dung,권태형,안용조 한국약용작물학회 2022 한국약용작물학회지 Vol.30 No.6
Background: In this study, we evaluated the antioxidant and anti-inflammatory effects of Oxalis corniculata hot water extract (OCWE) using lipopolysaccharide (LPS)-stimulated RAW264.7 cell. Methods and Results: Antioxidant activity was evaluated by measuring total polyphenol content, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity, 2,2′-azino-bis(3-ethylbenzothiazoline-6- sulfonic acid) radical scavenging activity, and reducing power ability. The total polyphenol content was found to be 33.06 ± 0.31 ㎎·gallic acid equivalent/g, and each evaluation showed reduction rates of 21.93 ± 2.43%, 64.93 ± 0.83%, and 0.260 ± 0.01 at the an OCWE concentration of 400 ㎍/㎖. To evaluate the anti-inflammatory effect of OCWE, a nitric oxide (NO) assay, reverse tras- cription polymerase chain reaction and western blotting were performed. At 400 ㎍/㎖ concentra- tion of OCWE, NO generation was reduced by 52.5 ± 1.37%, compared to LPS-stimulated RAW264.7 cells. OCWE also inhibited iNOS, COX-2, TNF-α, and IL-6 in at the protein ans mRNA levels in a dose-dependent manner. Conclusions: Taken together, these results indicate that OCWE had strong antioxidant and antiinflammatory effects. We suggest that OCWE may be an antioxidant and anti-inflammation agent in functional foods.
흑노호 줄기 추출물의 항산화 활성 및 산화적 손상에 따른 간세포보호 효과
한준희(Joon Hee Han),홍민(Min Hong),Hoang Viet Bach Khoa,Luong Van Dung,권태형(Tae Hyung Kwon),이수응(Soo Ung Lee) 한국약용작물학회 2024 한국약용작물학회지 Vol.32 No.1
Background: Reactive oxygen species (ROS) contribute to increased intracellular oxidative stress, which has detrimental effects on normal cells and reduces antioxidant levels. This oxidative stress, particularly in the liver, causes inflammation, which leads to the development of acute and chronic liver diseases. Hence, substances with the ability to efficiently reduce ROS levels and oxidative stress are needed. Methods and Results: Kadsura coccinea stem extract exhibited a concentration dependent antioxidant activity and imporved alcohol dehydrogenase and aldehyde dehydrogenase effects. Noncytotoxic effects and increased cell activity were observed in HepG2 cells treated with K. coccinea stem extract under conditions of hydrogen peroxide induced hepatotoxicity. Additionally, the extract effectively reduced ROS levels, as confirmed by 2′,7′-dichlorodihydrofluorescein diacetate (DCFHDA) assay in HepG2 cells simultaneously exposed to hydrogen peroxide and the K. coccinea stem extract. mRNA expression analysis revealed elevated levels of antioxidant enzymes, including superoxide dismutase 1 (SOD1), glutathione peroxidase 1 (GPx1), and catalase (CAT). Conclusions: These findings reveal the potential therapeutic value of K. coccinea stem extract for oxidative stress related liver disorders.Kadsura coccinea;Antioxidant;Ethanol Extract;Hepatoprotective Effects;Reactive Oxygen Species