Ecofriendly synthesis of silver and gold nanoparticles by <i>Euphrasia officinalis</i> leaf extract and its biomedical applications

Singh, Hina,Du, Juan,Singh, Priyanka,Yi, Tae Hoo Informa UK (Taylor Francis) 2018 Artificial cells, nanomedicine, and biotechnology Vol.46 No.6

Lysobacter agri sp. nov., a bacterium isolated from soil

Singh, Hina,Won, KyungHwa,Du, Juan,Yang, Jung-Eun,Akter, Shahina,Kim, Ki-Young,Yi, Tae-Hoo Springer-Verlag 2015 Antonie van Leeuwenhoek Vol.108 No.3

<P>A bacterial strain, designated as THG-SKA3(T), was isolated from field soil of Kyung Hee University, South Korea. Cells of the isolate were observed to be Gram-negative, aerobic, rod-shaped and motile by gliding. The strain was found to grow optimally at 28?C, at pH 7 and in absence of NaCl. Based on 16S rRNA gene sequence comparisons, strain THG-SKA3(T) shared highest sequence similarity with Lysobacter niastensis KACC 11588(T) followed by Lysobacter panacisoli KACC 17502(T), Lysobacter enzymogenes LMG 8762(T) and Lysobacter oryzae KCTC 22249(T). The G+C content of THG-SKA3(T) was determined to be 68.9?mol%. The DNA-DNA relatedness values between strain THG-SKA3(T) and its closest phylogenetic neighbors were below 25.0?%.The major polar lipids of strain THG-SKA3(T) were determined to be diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant respiratory quinone was identified as ubiquinone 8 (Q-8). The major cellular fatty acids were identified as branched chain iso-C15:0, iso-C16:0 and unsaturated iso-C17:1 ω9c. On the basis of polyphasic data presented, it is evident that strain THG-SKA3(T) represents a novel species of the genus Lysobacter, for which the name Lysobacter agri sp. nov. (type strain THG-SKA3(T)?=?KACC 18283(T)?=?CSCTCC AB 2015126(T)) is proposed.</P>

Novosphingobium aquaticum sp. nov., isolated from lake water in Suwon, Republic of Korea

Singh, Hina,Du, Juan,Yang, Jung-Eun,Yin, ChangShik,Kook, MooChang,Yi, Tae-Hoo Springer-Verlag 2015 Antonie van Leeuwenhoek Vol.108 No.4

Lysobacter fragariae sp. nov. and Lysobacter rhizosphaerae sp. nov. isolated from rhizosphere of strawberry plant.

Singh, Hina,Du, Juan,Ngo, Hien T T,Won, KyungHwa,Yang, Jung-Eun,Kim, Ki-Young,Yi, Tae-Hoo N.V. Swets en Zeitlinger 2015 Antonie van Leeuwenhoek Vol.107 No.6

<P>Two bacterial strains, designated THG-DN8.7(T) and THG-DN8.3(T), were isolated from the rhizosphere of a strawberry plant in Gyeryong Mountain, South Korea. Cells of both isolates were observed to be Gram-negative, yellow-coloured and rod-shaped. Comparative 16S rRNA gene sequence analysis showed that strain THG-DN8.7(T) had highest sequence similarities to Lysobacter yangpyeongensis KACC 11407(T) (97.2?%), Lysobacter niabensis KACC 11587(T) (97.0?%) and Lysobacter oryzae KCTC 22249(T) (96.9?%), while strain THG-DN8.3(T) had closely similarity with L. niabensis KACC 11587(T) (98.1?%), L. oryzae KCTC 22249(T) (97.1?%) and L. yangpyeongensis KACC 11407(T) (96.1?%). DNA-DNA relatedness values between strains THG-DN8.7(T) and THG-DN8.3(T) and their closest phylogenetically neighbours were below 30.0?%, which indicates that strains THG-DN8.7(T) and THG-DN8.3(T) represent distinct species within the genus Lysobacter. Both strains were found to contain iso-C15:0, iso-C16:0 and iso-C17:1 ω9c as predominant fatty acids and ubiquinone-8 as major isoprenoid quinone. The major polar lipids were identified as phosphatidylethanolamine, phosphatidyl-N-methylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content of strains THG-DN8.7(T) and THG-DN8.3(T) were determined to be 66.9 and 67.8?mol%, respectively. These data are consistent with the affiliation of the two new species represented by THG-DN8.7(T) and THG-DN8.3(T) to the genus Lysobacter. The names Lysobacter fragariae sp. nov. and Lysobacter rhizosphaerae sp. nov. are proposed for these species with the type strains THG-DN8.7(T) (=KCTC 42236(T)?=?JCM 30322(T)) and THG-DN8.3(T) (=KCTC 42237(T)?=?JCM 30321(T)), respectively.</P>

Flavobacterium vireti sp. nov., isolated from soil.

Singh, Hina,Du, Juan,Won, KyungHwa,Yang, Jung-Eun,Akter, Shahina,Kim, Ki-Young,Yi, Tae-Hoo N.V. Swets en Zeitlinger 2015 Antonie van Leeuwenhoek Vol.107 No.6

<P>A novel Gram-negative, aerobic, yellow-pigmented, non-motile and rod-shaped bacterium, designated as THG-SM1(T), was isolated from field soil collected from Suwon, South Korea. The strain was found to grow optimally at 28?C, at pH 7.0 and in the absence of NaCl. Based on 16S rRNA gene sequence similarities, strain THG-SM1(T) belongs to the genus Flavobacterium and is most closely related to Flavobacterium terrae KACC 11731(T), followed by Flavobacterium columnare KACC 11683(T) and Flavobacterium enshiense KCTC 23775(T). The DNA G+C content of the novel isolate was determined to be 38.5?mol%. In DNA-DNA hybridization tests, the DNA relatedness between strain THG-SM1(T) and its closest phylogenetic neighbour F. terrae was below 50?%. Flexirubin-type pigments were found to be present. The major polar lipid and isoprenoid quinone were phosphatidylethanolamine and menaquinone 6 (MK-6), respectively. The main cellular fatty acids were identified as iso-C15:1G, iso-C15:0 3OH, iso-C16:0 and iso-C15:0. The DNA-DNA hybridization result and differentiating chemotaxonomic and phenotypic characteristics showed that strain THG-SM1(T) represents a novel species of the genus Flavobacterium, for which the name Flavobacterium vireti sp. nov. is proposed. The type strain is THG-SM1(T) (=KACC 18371(T)?=?CCTCC AB2014312(T)).</P>

Biosynthesis, characterization, and antimicrobial applications of silver nanoparticles

Singh, Priyanka,Kim, Yeon Ju,Singh, Hina,Wang, Chao,Hwang, Kyu Hyon,Farh, Mohamed El-Agamy,Yang, Deok Chun Dove Medical Press 2015 INTERNATIONAL JOURNAL OF NANOMEDICINE Vol.10 No.-

<P>In the present study, the strain <I>Brevibacterium frigoritolerans</I> DC2 was explored for the efficient and extracellular synthesis of silver nanoparticles. These biosynthesized silver nanoparticles were characterized by ultraviolet-visible spectrophotometry, which detected the formation of silver nanoparticles in the reaction mixture and showed a maximum absorbance at 420 nm. In addition, field emission transmission electron microscopy revealed the spherical shape of the nanoparticles. The dynamic light scattering results indicated the average particle size of the product was 97 nm with a 0.191 polydispersity index. Furthermore, the product was analyzed by energy dispersive X-ray spectroscopy, X-ray diffraction, and elemental mapping, which displayed the presence of elemental silver in the product. Moreover, on a medical platform, the product was checked against pathogenic microorganisms including <I>Vibrio parahaemolyticus</I>, <I>Salmonella enterica</I>, <I>Bacillus anthracis</I>, <I>Bacillus cereus</I>, <I>Escherichia coli</I>, and <I>Candida albicans</I>. The nanoparticles demonstrated antimicrobial activity against all of these pathogenic microorganisms. Additionally, the silver nanoparticles were evaluated for their combined effects with the commercial antibiotics lincomycin, oleandomycin, vancomycin, novobiocin, penicillin G, and rifampicin against these pathogenic microorganisms. These results indicated that the combination of antibiotics with biosynthesized silver nanoparticles enhanced the antimicrobial effects of antibiotics. Therefore, the current study is a demonstration of an efficient biological synthesis of silver nanoparticles by <I>B. frigoritolerans</I> DC2 and its effect on the enhancement of the antmicrobial efficacy of well-known commercial antibiotics.</P>

Development of superparamagnetic iron oxide nanoparticles via direct conjugation with ginsenosides and its in-vitro study

Singh, Hina,Du, Juan,Singh, Priyanka,Mavlonov, Gafurjon Tom,Yi, Tae Hoo Elsevier 2018 Journal of photochemistry and photobiology. B, Bio Vol.185 No.-

<P><B>Abstract</B></P> <P>The current study focused on direct conjugation of superparamagnetic iron oxide nanoparticles (SPIONs) with ginsenosides CK and Rg3. The direct conjugation approach was low-cost, eco-friendly, simple, fast and high yield. The synthesized conjugates (SPION-CK and SPION-Rg3) were characterized by field emission transmission electron microscopy, dynamic light scattering, zeta potential, X-ray diffractometer, and magnetometer. The characterization results confirmed the formation of SPIONs conjugates. The maximum attaching percentage for ginsenosides to SPIONs was found to be 5%. In vitro cytotoxicity assay in HaCaT keratinocyte cells revealed that the conjugates were non-cytotoxic to normal cells. Moreover, the anti-inflammatory activity of SPION-CK and SPION-Rg3 were investigated. The expression of reactive oxygen species (ROS) in lipopolysaccharide-activated RAW 264.7 (murine macrophage cells) were inhibited by SPIONs conjugates in a dose-dependent manner. In addition, SPION-CK and SPION-Rg3 significantly reduced the production of nitric oxide and inducible nitric oxide synthase (iNOS) in a dose-dependent manner in the lipopolysaccharide-induced RAW 264.7 cells. Overall the results suggested that the SPIONs were conjugated with ginsenosides CK and Rg3 by using direct conjugation approach were non-cytotoxic and can be used as a carrier for intracellular release of ginsenosides in inflammatory diseases.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Simple, cheap, ecofriendly and high yield direct conjugation method has been developed. </LI> <LI> Direct conjugation method was used for superparamagnetic iron oxide nanoparticles (SPIONs) conjugation. </LI> <LI> Direct conjugation method was successful in conjugating SPIONs with ginsenosides CK and Rg3. </LI> <LI> The ginsenosides conjugates were nontoxic to HaCaT keratinocyte cells. </LI> <LI> The conjugates showed anti-inflammatory effects in RAW 264.7 cells. </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>Conjugation scheme of ginsenosides with superparamagnetic iron oxide nanoparticles (SPIONs).</P> <P>[DISPLAY OMISSION]</P>

Nocardioides albidus sp. nov., an actinobacterium isolated from garden soil

Singh, Hina,Du, Juan,Trinh, Huan,Won, KyungHwa,Yang, Jung-Eun,Yin, ChangShik,Kook, MooChang,Yi, Tae-Hoo Microbiology Society 2016 International journal of systematic and evolutiona Vol.66 No.-

<P>A novel bacterial strain, designated THG-S11.7(T), was isolated from garden soil in Incheon, South Korea. Cells of the strain were Gram-stain-positive, aerobic, non-motile cocci, and were catalase- and oxidase-positive. Colonies of the strain were white. Strain THG-S11.7(T) grew optimally at 28 degrees C, at pH 7.0 and in the presence of 2.0 % NaCl. 16S rRNA gene sequence analysis indicated that the strain was a member of the genus Nocardioides. Strain THG-S11.7(T) showed a 16S rRNA gene sequence similarity of 98.2 % to Nocardioides kongjuensis KCTC 19054(T), 98.0 % to Nocardioides caeni KCTC 19600(T), 97.9 % to Nocardioides daeguensis KCTC 19799(T), 97.8 % to Nocardioides nitrophenolicus KCTC 047BP(T), 97.6 % to Nocardioides aromaticivorans KACC 20613(T), 97.5 % to Nocardioides simplex KACC 20620(T) and 97.0 % to Nocardioides ginsengisoli KCTC 19135(T). DNA-DNA relatedness values between strain THG-S11.7(T) and the closest phylogenetic neighbours were below 45.0 % and the DNA G + C content of strain THG-S11.7(T) was 72.2 mol%. Strain THG-S11.7(T) was characterized chemotaxonomically as having LL-diaminopimelic acid in the cell-wall peptidoglycan and menaquinone MK-8(H-4) as the predominant isoprenoid quinone. The major phospholipid was determined to be diphosphatidylglycerol. The major cellular fatty acids of strain THG-S11.7(T) were iso-C-15 : 0, C-16 : 0 and iso-C-16 : 0. Based on the phenotypic, genotypic and phylogenetic analyses, it is proposed that the isolate represents a novel species of the genus Nocardioides, for which the name Nocardioides albidus sp. nov. is proposed. The type strain is THG-S11.7(T) (=KCTC 39607(T)=CCTCC AB 2015297(T)).</P>

Taibaiella yonginensis sp. nov., a bacterium isolated from soil of Yongin city.

Singh, Hina,Du, Juan,Won, KyungHwa,Yang, Jung-Eun,Akter, Shahina,Kim, Ki-Young,Yin, ChangShik,Yi, Tae-Hoo N.V. Swets en Zeitlinger 2015 Antonie van Leeuwenhoek Vol.108 No.3

<P>A novel bacterial strain, designated as THG-SC4(T), was isolated from a soil sample collected from Yongin city in South Korea. Cells of the strain were Gram-negative, aerobic, rod-shaped and non-motile. The strain grew optimally at 28-30?C; at pH 7.0 and in the absence of NaCl. Flexirubin-type pigments were found to be present. On the basis of 16S rRNA gene sequence similarity, strain THG-SC4(T) was shown to belong to the genus Taibaiella and shares high sequence similarity with Taibaiella smilacinae KCTC 32316(T) (95.4?%), followed by Taibaiella koreensis KACC 17171(T) (94.3?%) and Taibaiella chishuiensis JCM 19637(T) (94.2?%). The DNA G+C content of the novel isolate was determined to be 43.1?mol% and MK-7 was identified as the predominant isoprenoid quinone. The only polyamine was homospermidine. The major polar lipids were phosphatidylethanolamine, an unidentified aminophospholipid and an unidentified polar lipid. The predominant fatty acids were identified as iso-C15:0, iso-C15:1 G, C16:0 and iso-C17:03-OH. On the basis of data from this polyphasic taxonomic study, strain THG-SC4(T) is considered to represent a novel species of the genus Taibaiella, for which the name Taibaiella yonginensis sp. nov. is proposed. The type strain is THG-SC4(T) (=KACC 18372(T) = CCTCC AB 2014316(T)).</P>

Brachybacterium horti sp. nov., isolated from garden soil

Singh, Hina,Du, Juan,Yang, Jung-Eun,Shik Yin, Chang,Kook, MooChang,Yi, Tae-Hoo Microbiology Society 2016 International journal of systematic and evolutiona Vol.66 No.-

<P>A bacterial strain, THG-S15-4(T), was isolated from garden soil taken from the Guro-gu district of Seoul, Republic of Korea. Strain THG-S15-4(T). was Gram-stain-positive, facultatively anaerobic, coccus-shaped and non-motile, forming white colonies. The strain grew optimally at 25-37 degrees C, at pH 7.0 and in the presence of 0-2.0 % (w/v) NaCI. Phylogenetic analysis, based on 16S rRNA gene sequences, showed that strain THG-S15-4(T) was affiliated to species of the genus Brachybacterium, and the most closely related species were Brachybacterium rhamnosum KCTC 9917(T) (98.5 % sequence similarity) and Brachybacterium squillarum KCTC 19899(T) (96.9 % sequence similarity). The DNA DNA relatedness between strain THG-S15-4(T). and B. rhamnosum KCTC 9917(T) was found to be below 20.0 %. The DNA G+C content was determined to be 69.5 mol%. The major isoprenoid quinone detected was MK-7.Strain THG-S15-4(T). was characterized chemotaxonomically as having meso-diaminopimelic acid in the cell-wall peptidoglycan. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, unidentified glycolipids and an unidentified polar lipid. The major fatty acids were found to be iso-C-16 : 0 and anteiso-C-15 (:) (0). The results of physiological and biochemical tests enabled strain THG-S15-4(T) to be differentiated phenotypically from species of the genus Brachybacterium with validly published names. Therefore, it is suggested that this newly isolated organism represents a novel species, for which the name Brachybacterium horti sp. nov. is proposed. The type strain is THG-S15-4(T) (=KCTC 39563(T)=CCTCC AB 2015116(T)).</P>