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- 저자 : Heesch, Svenja (검색결과 2 건)
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Heesch, Svenja,Cho, Ga Youn,Peters, Akira F.,Le Corguillé,, Gildas,Falentin, Cyril,Boutet, Gilles,Coë,del, Solè,ne,Jubin, Claire,Samson, Gaelle,Corre, Erwan,Coelho, Susana M.,Mark Cock Blackwell Publishing Ltd 2010 The New phytologist Vol.188 No.1
<P><B>Summary</B></P><P><P> <I>Ectocarpus siliculosus</I> has been proposed as a genetic and genomic model for the brown algae and the 214 Mbp genome of this organism has been sequenced. The aim of this project was to obtain a chromosome‐scale view of the genome by constructing a genetic map using microsatellite markers that were designed based on the sequence supercontigs.</P><P>To map genetic markers, a segregating F<SUB>2</SUB> population was generated from a cross between the sequenced strain (Ec 32) and a compatible strain from northern Chile. Amplified fragment length polymorphism (AFLP) analysis indicated a significant degree of polymorphism (41%) between the genomes of these two parental strains. Of 1,152 microsatellite markers that were selected for analysis based on their location on long supercontigs, their potential as markers and their predicted ability to amplify a single genomic locus, 407 were found to be polymorphic.</P><P>A genetic map was constructed using 406 markers, resulting in 34 linkage groups. The 406 markers anchor 325 of the longest supercontigs on to the map, representing 70.1% of the genome sequence.</P><P>The <I>Ectocarpus</I> genetic map described here not only provides a large‐scale assembly of the genome sequence, but also represents an important tool for future genetic analysis using this organism.</P></P>
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Novita Intan Arovah,Kristiann C. Heesch 대한예방의학회 2020 Journal of Preventive Medicine and Public Health Vol.53 No.3
Objectives: The Short Form 36 (SF-36) questionnaire is increasingly being used to measure health-related quality of life (HRQoL) in Indonesia. However, evidence that it is valid for use in Indonesian adults is lacking. This study assessed the validity and reliability of the SF-36 in Indonesian middle-aged and older adults. Methods: Adults aged 46-81 years (n=206) in Yogyakarta, Indonesia completed the SF-36, another measure of HRQoL (the EuroQoL visual analogue scale [EQ-VAS]), and measures assessing their demographic characteristics. Fifty-four percent (n=121) completed the SF-36 measure again 1 week later. Confirmatory factor analysis was conducted to confirm the factor structure of the SF-36. Internal consistency reliability was estimated using Cronbach’s alpha, and test-retest reliability was assessed using intraclass correlations. Convergent and discriminant validity were assessed by computing correlations among SF-36 subscales, between subscales and the 2 component scores, and between component scores and EQ-VAS scores. Results: Most scaling assumptions were met. The hypothetical factor structure fit the data poorly (root mean square error of approximation [RMSEA]=0.108) and modification was required for a good fit (RMSEA=0.060). Scores on all subscales demonstrated acceptable internal consistency (α>0.70) and test-retest reliability (r>0.70). Divergent validity was supported by weak to moderate inter-scale correlations (r=0.19 to 0.64). As expected, the 2 summary scores were moderately to strongly correlated with the EQ-VAS (r>0.60). Conclusions: The findings adequately support the use of SF-36 in Indonesian middle-aged and older adults, although the optimal algorithm for computing component scores in Indonesia warrants further investigation.
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