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        A NOTE ON THE REMOVAL OF PHYTATE IN SOYBEAN MEAL USING Aspergillus usami

        Ilyas, A.,Hirabayasi, M.,Matsui, T.,Yano, H.,Yano, F.,Kikishima, T.,Takebe, M.,Hayakawa, K. Asian Australasian Association of Animal Productio 1995 Animal Bioscience Vol.8 No.2

        Soybean meal was fermented by Aspergillus usami in order to reduce phytate content. Aflatoxin B1 was not detected in the fermented soybean meal. The contents of crude protein, crude fiber, ether extract and crude ash were slightly increased following fermentation with a concomitant reduction in nitrogen free extract. Though the fermentation partly degraded proteins in the soybean meal, there was small difference in amino acid composition between the soybean meal and the fermented soybean meal. The results showed that the fermentation did not affect nutritional value of protein in soybean meal. Approximately 55% of phosphorus extracted by trichloroacetic acid was inositol hexaphosphate (phytate) in the soybean meal. The content of inositol tetra to hexaphosphates was not detected in the fermented soybean meal. These results indicated that the fermentation almost completely eliminated phytate in soybean meal. Phytase activity was not detected in the unfermented soybean meal. However, the enzyme activity in the fermented soybean meal was 167.7 U/g. When the fermented soybean meal in supplemented in formula feeds, phytase in the fermented soybean meal might partly degrade the phytate in other ingredients in the digestive tract. The fermented soybean meal is possibly used as a phytate-free protein source of feed, which contains high available phosphorus.

      • Performance of Fluidized Bed Gasification for Wood Biomass and Refused Paper/Plastics Fuel (RPF)

        ( Myo Min Win ),( Misuzu Asari ),( Ryo Hayakawa ),( Hiroyuki Hosoda ),( Junya Yano ),( Shin-ichi Sakai ) 한국폐기물자원순환학회(구 한국폐기물학회) 2018 한국폐기물자원순환학회 심포지움 Vol.2018 No.1

        Encouraging sound material cycles and waste management, the power generation from thermal wastetreatment facilities in small- and medium-scaleis considered as an important issue in Japan. This study intends to obtain a gasification data applying different kind of feedstock (wood biomass and waste) and consider the gasification performance, calorific conditions, and tar concentration level for syngas utilization in gas engine. We used the examples of biomass and waste streams (wood pellet and RPF) as feedstock. The gasification characteristics using fluidized bed gasifier (FBG) was obtained at a temperature range of 650-950℃ in varying equivalent ratios (ER) of 0.3, 0.4, and 0.5 with atmospheric air as the gasifying medium (Table 1). Here, ER of primary air was fixed in each conditions and ER of secondary air was adjusted to meet the test conditions. The product gas and tar were collected at the gasifier outlet and their composition and concentration were analyzed. As in the observed key gas compositions in relation to temperature (Figure 1a-b), carbon monoxide (CO) showed a decreasing trend for wood pellets and an increasing trend for RPF when the temperature goes higher. The CO concentration was significantly different between wood pellets and RPF at lower temperatures. H<sub>2</sub> yields were higher when using wood pellets compared to RPF, first declining before increasing with rising temperature. In the case of C2-C3 (C<sub>m</sub>H<sub>n</sub>), the concentration tendency was different between wood pellets and RPF, especially around 700℃. Lower heating value (LHV) is very important reflected by consistent gas composition for gas engines. At increased temperature, a decline in LHV was observed for both types of fuel but tar cracking was observed to be better (Figure 1c-d). It is important to operate the system in a way that maintains proper (LHV) while preserving the level of tars as sufficiently as possible in the FBG-generated gas. Following the technical examinations, we confirmed the temperature conditions for the applicable range of LHV in Runs 2 and 3 for wood pellets (4.3 and 4.7 MJ/Nm<sup>3</sup>, respectively) and Runs 4-6 for RPF (4.1, 5.1, and 5.9 MJ/Nm<sup>3</sup>, respectively). There are no common regulations for gas LHV with regards to gas engine for product gas, but 4.0 MJ/Nm<sup>3</sup> of LHV could be considered as one standard. Tar concentration tended to decrease as gasification temperature increased due to the apparent thermal cracking in the freeboard. When the LHV levels were held to the required limits in this study, the tar concentrations at the gasifier exit (at optimum ER) ranged from 4-14 g/Nm<sup>3</sup>-dry. The dew point of the gas can also be appropriately cited in identifying the tar concentration for engine applications. Realizing the applicable LHV performance, the ER range could be optimized at around 0.3-0.4 in our gasification test for mixed-plastic waste, RPF, and biomass in this study to be utilized in gas engines. An optimal ER of up to 0.4 was perceived considering LHV levels maintaining the required limits with a tar concentration to be sufficiently low at the gasifier outlet, such that further tar reduction by in process thermal/catalytic cracking or downstream post treatment would meet gas engine acceptable tolerance. In this study, we could investigate the feasibility of air-only gasifying mediums with regards to both the inherent advantages of small-scale systems and the potential economic benefits.

      • An alternative spliced mouse presenilin-2 mRNA encodes a novel γ-secretase inhibitor

        Suzuki, Y.,Ohta, K.,Itoh, M.,Sakoh-Sumitomo, Y.,Mitsuda, T.,Ueda, M.,Hayakawa-Yano, Y.,Li, S.,Hida, Y.,Inuzuka, T.,Jung, Y.K.,Nakagawa, T. North-Holland Pub ; Elsevier Science Ltd 2009 FEBS letters Vol.583 No.9

        The γ-secretase, composed of presenilin-1 (PS1) or presenilin-2 (PS2), nicastrin (NCT), anterior pharynx-defective phenotype 1 (APH-1), and PEN-2, is critical for the development of Alzheimer's disease (AD). PSs are autoproteolytically cleaved, producing an N-terminal fragment (NTF) and a hydrophilic loop domain-containing C-terminal fragment. However, the role of the loop domain in the γ-secretase complex assembly remains unknown. Here, we report a novel PS2 isoform generated by alternative splicing, named PS2β, which is composed of an NTF with a hydrophilic loop domain. PS2β disturbed the interaction between NCT and APH-1, resulting in the inhibition of amyloid-β production. We concluded that PS2β may inhibit γ-secretase activity by affecting the γ-secretase complex assembly. Structured summary: MINT-7025654: APH1 (uniprotkb:Q96BI3) physically interacts (MI:0218) with PEN2 (uniprotkb:Q9NZ42), PS2 beta (uniprotkb:Q61144-2) and PS1 (uniprotkb:P49769) by anti tag coimmunoprecipitation (MI:0007) MINT-7025631: APH1 (uniprotkb:Q96BI3) physically interacts (MI:0218) with NCT (uniprotkb:Q92542), PEN2 (uniprotkb:Q9NZ42) and PS1 (uniprotkb:P49769) by anti tag coimmunoprecipitation (MI:0007)

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