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        Hair follicle development and related gene and protein expression of skins in Rex rabbits during the first 8 weeks of life

        Zhenyu Wu,Liangzhan Sun,Gongyan Liu,Hongli Liu,Hanzhong Liu,Zhiju Yu,Shuang Xu,Fuchang Li,Yingbe Qin 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.4

        Objective: We aimed to observe hair follicle (HF) development in the dorsal skin and elucidate the expression patterns of genes and proteins related to skin and HF development in Rex rabbits from birth to 8 weeks of age. Methods: Whole-skin samples were obtained from the backs of Rex rabbits at 0, 2, 4, 6, and 8 weeks of age, the morphological development of primary and secondary HFs was observed, and the gene transcript levels of insulin-like growth factor-I (IGF-I), epidermal growth factor (EGF), bone morphogenetic protein 2 (BMP2), transforming growth factor β-1, 2, and 3 (TGFβ-1, TGFβ-2, and TGFβ-3) were examined using quantitative real-time polymerase chain reaction (PCR). Additionally, Wnt family member 10b (Wnt10b) and β-Catenin gene and protein expression were examined by quantitative real-time PCR and western blot, respectively. Results: The results showed significant changes in the differentiation of primary and secondary HFs in Rex rabbits during their first 8 weeks of life. The IGF-I, EGF, TGFβ-2, and TGFβ-3 transcript levels in the rabbits were significantly lower at 2 weeks of age than at birth and gradually increased thereafter, while the BMP2 and TGFβ-1 transcript levels at 2 weeks of age were significantly higher than those at birth and gradually decreased thereafter. β-Catenin gene expression was also significantly affected by age, while the Wnt10b transcript level was not. However, the Wnt10b and β-catenin protein expression levels were the lowest at 2 and 4 weeks of age. Conclusion: Our data showed that a series of changes in HFs in dorsal skin occurred during the first 8 weeks. Many genes, such as IGF-I, EGF, BMP2, TGFβ-1, TGFβ-2, TGFβ-3, and β-Catenin, participated in this process, and the related proteins Wnt10b and β-Catenin in skin were also affected by age.

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        Compensation-based Characteristic Modeling and Tracking Control for Electromechanical Servo Systems With Backlash and Torque Disturbance

        Xiang Wang,Hanzhong Liu,Jiali Ma,Yang Gao,Yifei Wu 제어·로봇·시스템학회 2024 International Journal of Control, Automation, and Vol.22 No.6

        For two-inertia servo mechanisms, the high order, backlash nonlinearity and external disturbance make the precise modeling and control difficult to implement. This article provides an easily implemented modeling and control strategy to deal with this problem. A characteristic modeling framework of disturbed nonlinear systems is proposed. To restrain the modeling error, the backlash nonlinearity and torque disturbance are observed by constructing a finite-time extended state observer (FESO) based on homogeneity properties and then the compensation action can be taken. Based on the compensated system, the discrete-time characteristic model is established using the sampled input-output data, which degrades the modeling complicacy. To estimate the model parameters, an adaptation law with projection algorithm is proposed using the tracking error and the estimation error as the excitation signal. A discrete-time second-order fast terminal sliding-mode control (DSFTSC) is proposed based on the characteristic model to stabilize the whole system, where an improved reaching law is designed to enhance the rapidity and weaken the chattering and the utilization of the fast terminal switching surface also speeds up the regression rate and decreases the tracking error. Finally, the effectiveness of the characteristic modeling, the adaptive law and the control scheme is validated by simulations in Matlab and experiments in a practical test rig, respectively.

      • The genome of the mesopolyploid crop species Brassica rapa

        Wang, Xiaowu,Wang, Hanzhong,Wang, Jun,Sun, Rifei,Wu, Jian,Liu, Shengyi,Bai, Yinqi,Mun, Jeong-Hwan,Bancroft, Ian,Cheng, Feng,Huang, Sanwen,Li, Xixiang,Hua, Wei,Wang, Junyi,Wang, Xiyin,Freeling, Michael Nature Publishing Group, a division of Macmillan P 2011 Nature genetics Vol.43 No.10

        We report the annotation and analysis of the draft genome sequence of Brassica rapa accession Chiifu-401-42, a Chinese cabbage. We modeled 41,174 protein coding genes in the B. rapa genome, which has undergone genome triplication. We used Arabidopsis thaliana as an outgroup for investigating the consequences of genome triplication, such as structural and functional evolution. The extent of gene loss (fractionation) among triplicated genome segments varies, with one of the three copies consistently retaining a disproportionately large fraction of the genes expected to have been present in its ancestor. Variation in the number of members of gene families present in the genome may contribute to the remarkable morphological plasticity of Brassica species. The B. rapa genome sequence provides an important resource for studying the evolution of polyploid genomes and underpins the genetic improvement of Brassica oil and vegetable crops.

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