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      저자 : Han Xiaoshuang (검색결과 2 건)
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        Accuracy evaluation of 3D time-domain Green function in infinite depth

        Zhang Teng,Zhou Bo,Li Zhiqing,Han Xiaoshuang,Gho Wie Min 대한조선학회 2021 International Journal of Naval Architecture and Oc Vol.13 No.1

        An accurate evaluation of three-dimensional (3D) Time-Domain Green Function (TDGF) in infinite water depth is essential for ship’s hydrodynamic analysis. Various numerical algorithms based on the TDGF properties are considered, including the ascending series expansion at small time parameter, the asymptotic expansion at large time parameter and the Taylor series expansion combines with ordinary differential equation for the time domain analysis. An efficient method (referred as “Present Method”) for a better accuracy evaluation of TDGF has been proposed. The numerical results generated from precise integration method and analytical solution of Shan et al. (2019) revealed that the “Present method” provides a better solution in the computational domain. The comparison of the heave hydrodynamic coefficients in solving the radiation problem of a hemisphere at zero speed between the “Present method” and the analytical solutions proposed by Hulme (1982) showed that the difference of result is small, less than 3%.

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        Single-Cell RNA Sequencing Shows T-Cell Exhaustion Landscape in the Peripheral Blood of Patients with Hepatitis B Virus-Associated Acute-on-Chronic Liver Failure

        ( Jia Yao ),( Yaqiu Ji ),( Tian Liu ),( Jinjia Bai ),( Han Wang ),( Ruoyu Yao ),( Juan Wang ),( Xiaoshuang Zhou ) 대한소화기기능성질환·운동학회 2024 Gut and Liver Vol.18 No.3

        Background/Aims: The occurrence and development of hepatitis B virus-associated acute-on-chronic liver failure (HBV-ACLF) is closely related to the immune pathway. We explored the heterogeneity of peripheral blood T cell subsets and the characteristics of exhausted T lymphocytes, in an attempt to identify potential therapeutic target molecules for immune dysfunction in ACLF patients. Methods: A total of 83,577 T cells from HBV-ACLF patients and healthy controls were screened for heterogeneity by single-cell RNA sequencing. In addition, exhausted T-lymphocyte subsets were screened to analyze their gene expression profiles, and their developmental trajectories were investigated. Subsequently, the expression of exhausted T cells and their capacity in secreting cytokines (interleukin 2, interferon γ, and tumor necrosis factor α) were validated by flow cytometry. Results: A total of eight stable clusters were identified, among which CD4<sup>+</sup> TIGIT<sup>+</sup> subset and CD8<sup>+</sup> LAG-3<sup>+</sup> subset, with high expression of exhaust genes, were significantly higher in the HBV-ACLF patients than in normal controls. As shown by pseudotime analysis, T cells experienced a transition from naïve T cells to effector T cells and then exhausted T cells. Flow cytometry confirmed that the CD4<sup>+</sup>TIGIT<sup>+</sup> subset and CD8<sup>+</sup>LAG-3<sup>+</sup> subset in the peripheral blood of the ACLF patients were significantly higher than those in the healthy controls. Moreover, in vitro cultured CD8<sup>+</sup>LAG-3<sup>+</sup> T cells were significantly fewer capable of secreting cytokines than CD8<sup>+</sup>LAG-3<sup>-</sup> subset. Conclusions: Peripheral blood T cells are heterogeneous in HBV-ACLF. The exhausted T cells markedly increase during the pathogenesis of ACLF, suggesting that T-cell exhaustion is involved in the immune dysfunction of HBV-ACLF patients. (Gut Liver 2024;18:520-530)

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