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- 저자 : Hajime Ohgushi (검색결과 2 건)
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Clinical Application of Marrow Mesenchymal Stem Cells for Hard Tissue Repair
Hajime Ohgushi,Shigeyuki Kitamura,Noriko Kotobuki,Motohiro Hirose,Hiroko Machida,Kaori Muraki,Yoshinori Takakura 연세대학교의과대학 2004 Yonsei medical journal Vol.45 No.SUP
Human marrow mesenchymal stem cells were cultured in a medium containing glycerophosphate, ascorbic acid, and dexamethasone (Dex) on alumina ceramic discs and on tissue culture polystyrene (TCPS) dishes. Cell proliferation followed by osteogenic differentiation was observed to be equal on both culture substrata. The differentiation resulted in the appearance of bone-forming osteoblasts, which fabricated mineralized matrices on these substrata. Stem cells kept at 4℃ for 24 h outside a CO2 incubator maintained a viability level of more than 90%. The regenerative cultured bone outside the incubator also maintained high alkaline phosphatase activity for several hours. These results verified that cultured bone fabricated at a cell processing center can be transported to distant hospitals for use in hard tissue repair. To date, the tissue engineered cultured bone formed on alumina ceramics in this environment have already been used in clinical situations, such as total ceramic ankle replacements.
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Zinc-containing tricalcium phosphate and related materialsfor promoting bone formation
Atsuo Ito,Makoto Otsuka,Haruo Kawamura,Masako Ikeuchi,Hajime Ohgushi,Yu Sogo,Noboru Ichinose 한국물리학회 2005 Current Applied Physics Vol.5 No.5
Calcium phosphate is a suitable carrier of zinc, an essential element that has stimulatory effects on bone formation in vitro and in vivo as well as an inhibitory effect on osteoclastic bone resorption in vitro. The highest zinc content is attained in b-tricalcium phosphate, where the zinc content reaches 6 wt%. Both rat and human bone marrow cells (BMCs) cultured on zinc-containing b-tricalcium phosphate and hydroxyapatite composite ceramics differentiated more than BMCs cultured on zinc-free composite ceramics in the presence of b-glycerophosphate and dexamethasone. The acceptable dose of zinc was higher for human BMCs than for rat BMCs. The solubility of ZnTCP, which contains a nontoxic level of zinc, decreased to 52–92% that of pure TCP in the pH range of 5.0–7.4. However, the resorbed volume of ZnTCP was much lower than that expected from the in vitro solubility of ZnTCP, becoming as low as 26–20% that of TCP, which indicates that the reduction in the resorbability of ZnTCP would be attributable principally to its lowered cellular activation property relative to that associated with pure TCP. Probably due to the lowered cellular activation property associated with ZnTCP, bone loss at the bone-implant interface was significantly arrested in the longterm implantation of ZnTCP/HAP. The intramuscular injection of ZnTCP powder is effective in increasing bone mineral density in the vicinity of the injected site in osteopenic animals. All these findings suggest that zinc-containing calcium phosphate is a biomaterial that promotes bone formation.
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