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Seeing the Previously Unseen: Biomedical Optics and Optical Metrology
H. Yoo(유홍기) Korean Society for Precision Engineering 2021 한국정밀공학회 학술발표대회 논문집 Vol.2021 No.11월
Recent advances in lasers, electronics, fiber optics, optical components, and signal and image processing techniques have introduced optical instrumentations for use in a variety of biomedical and industrial applications. In particular, confocal microscopy offers several advantages over conventional wide field optical microscopy, including the ability to control depth of field, the capability to collect serial optical sections from thick specimens, and the acquisition of three-dimensional images. Since confocal microscopy can provide extremely high-quality and three-dimensional images, it is widely used in various fields such as biological imaging and industrial measurement. In this talk, we discuss real-time three-dimensional imaging based on advanced confocal detection methods. In addition, multimodal multiphoton microscopy for precise tissue characterization is presented. Optical coherence tomography (OCT) acquires high-resolution tomographic images of biological systems. Especially, miniaturized fiber-based endoscopic OCT probe has been successfully translated into clinical diagnostics, including cardiology. While gray-scale OCT provides only the microstructural information of biological samples, multimodal endoscopic imaging techniques can provide comprehensive information, such as biochemical composition and molecular information, on top of the microstructure. We will cover the technical advancements of various fiber-based micro-endoscopy technologies and its biomedical applications.