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      • KCI등재

        Expression of the IKr components KCNH2 (rERG) and KCNE2 (rMiRP1) during late rat heart development

        K. R. J. Chun,M. Koenen,H. A. Katus,J. Zehelein 생화학분자생물학회 2004 Experimental and molecular medicine Vol.36 No.4

        To understand molecular mechanisms that regulate formation and maintenance of cardiac IKr (rapidly activating component of the delayed rectifier K+ curent), we have investigated the spatiotemporal expresion patern of two rat po-tassium voltage-gated channels, namely sub-family H (eag-related), member2 (KCNH2) (alias name: rERG) and Isk-related family, member2 bryonic development by means of the in situ hybridization technique. KCNE2 is transcribed predominantly in atrial und ventricular myocar-dium at stages E14.5-E18.5dpc and only a minor signal emerged in the tongue at E16.5dpc. In contrast, KCNH2 transcripts appeared in a les confined patern with intense signals in atrial and ventricular myocardium, somites, spinal cord, bowel system, central nervous system and thymus at stages E14.5-E18.5dpc. Non-cardiac expresion even exceeds the intensity of the cardiac signal, indicating that KCNH2 contri-butes to K+ curents in non-cardiac tisue as well. Transcription of the rat β-subunit KCNE2 is present in all regions of the fetal myocardium and co-distributes perfectly with transcription of the pore forming α-subunit KCNH2. It seems likely that KCNH2 and KCNE2 are linked to form cardiac IKr channels, asociated to cardiogene-sis and cardiomyocyte excitability.

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