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Calculation of the Allowable Drainage of Parallel Tunnels Based on Ecological Environment Protection
Helin Fu,Pengtao An,Guowen Cheng,Shujie Wen,Jie Li 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.5
To determine a reasonable value of parallel tunnel drainage, a steady-flow calculation model is established. The differential equilibrium equation of seepage is established, and the equation of the falling funnel curve in a steady state with drainage parameters is solved by using boundary conditions. The maximum drawdown depth of the control point is used to calculate the allowable discharge of the tunnel. Then, the curve equation of the falling funnel is determined, the volume of the drainage funnel is calculated, and the total discharge in the steady state is calculated according to the water storage coefficient. Finally, the theoretical formula is verified based on an engineering case and numerical simulation. Considering the actual engineering geology and hydrogeological conditions of the tunnel site, the total drainage, allowable drainage, and initial support bearing head height calculated with the proposed method are different from the actual measurements collected on site by 10%, 18.9% and 13.6%, respectively. Therefore, the calculation method can provide a reference for similar engineering.
Metabolic Characteristic of the Liver of Dairy Cows during Ketosis Based on Comparative Proteomics
Xu, Chuang,Wang, Zhe,Liu, Guowen,Li, Xiaobing,Xie, Guanghong,Xia, Cheng,Zhang, Hong You Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.7
The objective of the present study was to identify differences in the expression levels of liver proteins between healthy and ketotic cows, establish a liver metabolic interrelationship of ketosis and elucidate the metabolic characteristics of the liver during ketosis. Liver samples from 8 healthy multiparous Hostein cows and 8 ketotic cows were pooled by health status and the proteins were separated by two-dimensional-electrophoresis (2D-E). Statistical analysis of gels was performed using PDQuest software 8.0. The differences in the expression levels of liver proteins (p<0.05) between ketotic and healthy cows were identified by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF-TOF) tandem mass spectrometry. Five enzymes/proteins were identified as being differentially expressed in the livers of ketotic cows: expression of 3-hydroxyacyl-CoA dehydrogenase type-2 (HCDH), acetyl-coenzyme A acetyltransferase 2 (ACAT) and elongation factor Tu (EF-Tu) were down-regulated, whereas that of alpha-enolase and creatine kinase were up-regulated. On the basis of this evidence, it could be presumed that the decreased expression of HCDH, which is caused by high concentrations of acetyl-CoA in hepatic cells, in the livers of ketotic cows, implies reduced fatty acid ??oxidation. The resultant high concentrations of acetyl-CoA and acetoacetyl CoA would depress the level of ACAT and generate more ??hydroxybutyric acid; high concentrations of acetyl-CoA would also accelerate the Krebs Cycle and produce more ATP, which is stored as phosphocreatine, as a consequence of increased expression of creatine kinase. The low expression level of elongation factor Tu in the livers of ketotic cows indicates decreased levels of protein synthesis due to the limited availability of amino acids, because the most glucogenic amino acids sustain the glyconeogenesis pathway; thus increasing the level of alpha-enolase. Decreased protein synthesis also promotes the conversion of amino acids to oxaloacetate, which drives the Krebs Cycle under conditions of high levels of acetyl-CoA. It is concluded that the livers of ketotic cows possess high concentrations of acetyl-CoA, which through negative feedback inhibited fatty acid oxidation; show decreased fatty acid oxidation, ketogenesis and protein synthesis; and increased gluconeogenesis and energy production.