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Improving the Microbial Production of Amino Acids: From Conventional Approaches to Recent Trends
Guoqing Zhang,Xueni Ren,Xiuhong Liang,Yaqun Wang,Dexin Feng,Yujun Zhang,Mo Xian,Huibin Zou 한국생물공학회 2021 Biotechnology and Bioprocess Engineering Vol.26 No.5
Variable industrial strains have been applied in the fermentation of bulk amino acids. Strain discovery and evolution, process optimization are traditional approaches to improve the yield and efficiency of the bio-production process, hence to compete with chemical or enzymatic process in amino acids production. With the fast development of bioengineering and synthetic biology, the strains can be rationally engineered to achieve better performance and gain the capacity in the fermentation of broader range of amino acids, especially for value-added amino acids. This proposed review aims to summarize traditional and recent strains in the microbial production of amino acids, characterize their metabolic pathways and present potential objectives for rational evolution. In addition, this proposed review prospect the recent opportunities and challenges in the microbial production of value-added amino acids (rare amino acids, non-canonical amino acids, and unnatural amino acids).
Multiplicity results for a class of second order superlinear difference systems
Guoqing Zhang,Sanyang Liu 대한수학회 2006 대한수학회보 Vol.43 No.4
Using Minimax principle and Linking theorem in crit-ical point theory, we prove the existence of two nontrivial solutionsfor the following second order superlinear dierence systems(P)8<:2x(k 1) + g(k;y(k)) = 0; k2 [1;T];2y(k 1) + f(k;x(k)) = 0; k2 [1;T];x(0) = y(0) = 0;x(T + 1) = y(T + 1) = 0 ;where T is a positive integer, [1,T] is the discrete intervalf1;2;:;Tg; x(k) = x(k + 1) x(k) is the forward dierence operator and42x(k) = 4 (4 x(k)).
Development of Innovative Work Behavior Scale for PE Teachers in Primary and Middle Schools
( Guoqing Zhang ),( Jun Li ),( Chuan Yi ) 아시아스포츠융합과학회 2021 Asia Pacific Journal of Applied Sport Sciences Vol.2 No.2
PURPOSE This study analyzes the structure of innovative work behavior of PE teachers in primary and middle schools and creates a scale for assessing the innovative work behavior of PE teachers in primary and middle schools. METHOD In this study, questionnaires were combined literature analysis and behavioral event interview. The questionnaire was used for three surveys, and the number of valid questionnaires was 104, 232 and 267 respectively. The reliability and validity of the scale were tested. RESULT The innovative work behavior of PE teachers in primary and middle schools includs three factors: the innovative idea generation, the innovative idea promotion, and the innovative idea realization, and the cumulative explanatory variance of the three factors is 71.631%. The innovative work behavior scale of physical education teachers in primary and secondary schools has good internal consistency reliability, retest reliability, content validity, and structure validity. CONCLUSION The innovative work behavior of PE teachers in primary and middle schools is a multi-dimensional structure composed of three factors. The scale used in this study can be an effective tool to evaluate the innovative work behavior of PE teachers in primary and middle schools.
Guoqing Zhang,Kyungsik Kim 아시아스포츠융합과학회 2022 Asia Pacific Journal of Applied Sport Sciences Vol.3 No.3
PURPOSE The purpose of this study is to verify the fit of the behavioral loyalty (BL) model for customers in commercial sport centers in Hunan Province, China, and verify the relationship between sport service quality (SSQ), perceived value (PV), satisfaction (S) and BL. METHOD The research subject is the customers of the commercial sport centers in Hunan Province, China. A total of 995 questionnaires were collected and after deleting 28 incomplete or incorrect questionnaires, 967 questionnaires were retained for data analysis. We used CFA to validate the measurement model and SEM for hypothesis testing. RESULT First, SSQ influences customers’ PV and S but does not influence BL. That is to say, a high level of SSQ in a sport centers can improve customers’ PV and S. Second, PV influences BL and S, and S also affects BL. In other words, a high level of PV can improve S and BL. CONCLUSION The results of this research provide some effective suggestions for the sport industry of commercial sport centers, and provide a reference direction for the future sport industry research field.
Multiple periodic solutions for eigenvalue problems with a p-Laplacian and non-smooth potential
Guoqing Zhang,Sanyang Liu 대한수학회 2011 대한수학회보 Vol.48 No.1
In this paper, we establish a multiple critical points theorem for a one-parameter family of non-smooth functionals. The obtained result is then exploited to prove a multiplicity result for a class of periodic eigenvalue problems driven by the p-Laplacian and with a non-smooth potential. Under suitable assumptions, we locate an open subinterval of the eigenvalue.
MULTIPLE PERIODIC SOLUTIONS FOR EIGENVALUE PROBLEMS WITH A p-LAPLACIAN AND NON-SMOOTH POTENTIAL
Zhang, Guoqing,Liu, Sanyang Korean Mathematical Society 2011 대한수학회보 Vol.48 No.1
In this paper, we establish a multiple critical points theorem for a one-parameter family of non-smooth functionals. The obtained result is then exploited to prove a multiplicity result for a class of periodic eigenvalue problems driven by the p-Laplacian and with a non-smooth potential. Under suitable assumptions, we locate an open subinterval of the eigenvalue.
MULTIPLICITY RESULTS FOR A CLASS OF SECOND ORDER SUPERLINEAR DIFFERENCE SYSTEMS
Zhang, Guoqing,Liu, Sanyang Korean Mathematical Society 2006 대한수학회보 Vol.43 No.4
Using Minimax principle and Linking theorem in critical point theory, we prove the existence of two nontrivial solutions for the following second order superlinear difference systems $$(P)\{{\Delta}^2x(k-1)+g(k,y(k))=0,\;k{\in}[1,\;T],\;{\Delta}^2y(k-1)+f(k,\;x(k)=0,\;k{\in}[1,\;T],\;x(0)=y(0)=0,\;x(T+1)=y(T+1)=0$$ where T is a positive integer, [1, T] is the discrete interval {1, 2,..., T}, ${\Delat}x(k)=x(k+1)-x(k)$ is the forward difference operator and ${\Delta}^2x(k)={\Delta}({\Delta}x(k))$.
Xianku Zhang,Guoqing Zhang 제어·로봇·시스템학회 2016 International Journal of Control, Automation, and Vol.14 No.4
This study is concerned with robust adaptive fuzzy stabilization of the nonlinearly parameterized andperiodically time-varying system with known period of the parameterized disturbance. In this note, a novel uncertaintydelivering approach is developed to overcome the problems: “the initial evident oscillating error”, “explosionof complexity” and “curse of dimensionality” from the root causes that are common in the current literatures onapproximation-based algorithms. Furthermore, a novel adaptive fuzzy control scheme is proposed with advantagesof concise structure and easy-to-implementation in practical engineering. Only one fuzzy type approximator isintroduced to compensate uncertainties of the whole system in the scheme. Based on small gain theory, all signalsof the composite closed-loop system are guaranteed to be uniformly ultimately bounded. Finally, three simulationexperiments are presented to demonstrate the effectiveness of our results.
Xiao Tang,Kun Tong,Lishan Zhu,Guoqing Fu,Wei Chang,Ting Zhou,Zhibing Zhang,Ling Tong,Ling Zhang,Yuqin Shi,Ling Zhang,Yuqin Shi 대한독성 유전단백체 학회 2016 Molecular & cellular toxicology Vol.12 No.4
Di-2-ethylhexyl phthalate (DEHP) which belongs to phthalatic-acid esters, is a kind of harmful, global environmental pollutants. It is a known endocrine disrupting chemical and male reproductive toxicant. However, the mechanism by which DEHP exposure result in male reproductive toxicity is still unclear. To elucidate the productive toxicity mechanism of DEHP, we attemptted to investigate oxidative stress, apoptotic effects, mRNA and protein expression of apoptosis-associated genes including FasL, caspase-3, and caspase-8 in GC-2spd (mouse spermatogenic cells). The results showed that, with the increase of DEHP concentration, cell apoptosis rate increased; the activities of relation index of oxidative stress such as malondialdehyde (MDA), superoxide dismutase (SOD) and glutatione peroxidase (GSH-PX) changed significantly; the mRNA and protein expression levels of FasL, caspase-3, -8, altered obviously. These results suggested that DEHP could induce apoptosis of GC-2spd cells through oxidative stress and FasL-dependent pathway.