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- 검색키워드
- 저자 : Guoqiang Cao (검색결과 3 건)
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Drought stress-induced changes of microRNAs in diploid and autotetraploid Paulownia tomentosa
Guoqiang Fan,Xibing Cao,Lin Cao,Minjie Deng,Zhenli Zhao,Suyan Niu,Zhe Wang,Yuanlong Wang 한국유전학회 2017 Genes & Genomics Vol.39 No.1
Drought stress adversely affects plant productivity. Growth and timber production of Paulownia trees are limited under drought stress. Changes in gene expression patterns and miRNA in different ploidy of Paulownia tomentosa have been investigated. However, the responses of P. tomentosa to drought stress at the microRNA (miRNA) level have not been reported so far. To identify miRNA candidates and their target genes involved in the drought stress response in diploid and tetraploid P. tomentosa, four small RNA and four degradome libraries from diploid and autotetraploid P. tomentosa under normal and drought stress conditions were constructed and sequenced. A total of 41 conserved and 90 novel miRNAs were identified. Among these miRNAs, 67 (26 conserved and 41 novel) and 53 (six conserved and 47 novel) were significantly differentially expressed in response to drought stress in diploid and autotetraploid P. tomentosa, respectively. Degradome analysis identified 356 candidate miRNA target genes that encoded proteins with functions that included plant defense, transcriptional regulation, and hormone metabolism. In particular, miR4 and miR156 were identified only in autotetraploid P. tomentosa under drought stress. These results will help us build a foundation for future studies of the biological functions of miRNAmediated gene regulation in P. tomentosa.
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Fuzzy adaptive PID control method for multi-mecanum-wheeled mobile robot
Guoqiang Cao,Xinyu Zhao,Changlong Ye,Suyang Yu,Bangyu Li,Chunying Jiang 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.4
Mecanum-wheeled mobile robots are widely used because they can easily realize omnidirectional movement and have flexible movement characteristics. However, existing mecanum-wheeled robots generally have problems of low movement accuracy and poor stability. Structural optimization method can improve the movement accuracy and stability of mecanum-wheeled robots, but it cannot solve the problem fundamentally. In this study, the method of geometric modeling is applied to deduce the parameters kinematic error e, slip factor μ, and overturning angle λ, all of which have an effect on the movement accuracy and stability of the robot. Combined with the advantages of PID and fuzzy control, a fuzzy adaptive PID control method is proposed. The experimental prototype of the eight-mecanum-wheeled omnidirectional mobile robot is built to carry out the trajectory tracking control experiment, and the superiority in the fuzzy adaptive PID control method is verified.
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Deyu Xu,Jing Zhao,Guoqiang Cao,Jinyu Wang,Qinggang Li,Ping Zheng,Shuxin Zhao,Jibin Sun 한국생물공학회 2018 Biotechnology and Bioprocess Engineering Vol.23 No.1
Phosphoenolpyruvate carboxylase (PEPC) catalyzes the carboxylation of phosphoenolpyruvate (PEP) in the presence of bicarbonate to form oxaloacetate (OAA), and it plays an important role in high-efficient production of OAA-derived metabolites such as lysine, glutamate and succinate. However, PEPCs often suffered from serious feedback inhibition by various metabolic effectors like aspartate. Here, the feedback inhibition of PEPC from Corynebacterium glutamicum was removed by adding a short terminal peptide like His-tag. The effect of His-tag location on the structure and important properties such as activity, thermostability and feedback inhibition of PEPC has been investigated. The purified untagged PEPC, Nterminal His-tagged PEPC (PEPC-N-His) and C-terminal His-tagged PEPC (PEPC-C-His) were characterized. PEPCN- His (439.71/sec/mM) showed a 1.26 and 186-fold higher catalytic efficiency than untagged PEPC (348.59/sec/mM) and PEPC-C-His (2.36/sec/mM), respectively. Both PEPCN- His and untagged PEPC were significantly inhibited by aspartate at the concentrations above 4 mM (residual activities < 10%), while PEPC-C-His was almost desensitized to aspartate within 10 mM (around 90% of residual activity). Structural analysis showed that the extension of C-terminus may cause steric hindrance for aspartate binding with enzymes, leading to the deregulation of feedback inhibition of PEPC-C-His. This study provides a deeper understanding of the effect of terminal fragments on the structure and function of PEPCs, and helps to engineer the feedback inhibition of PEPCs and structurally similar enzymes.
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