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- 저자 : Guochao Shi (검색결과 3 건)
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Guochao Shi,Mingli Wang,Yanying Zhu,Xiaoya Yan, Siye Pan,Siye Pan,Anqi Zhang 한국물리학회 2019 Current Applied Physics Vol.19 No.11
An effective SERS-based detection method has been developed to quantitatively diagnose the goat serum which overcomes the problem of diffusion limitation in traditional heterogeneous immunoassay. In this work, the ultrasensitive silver/anodic aluminum oxide (Ag/AAO) SERS platform was explored via magnetron sputtering which can precisely control the sample morphology and intergap distances. Results indicated that the localized surface plasmon resonance (LSPR) effect was sharply strengthened as the sub-10 nm nanogaps generated and the enhancement factor (EF) for crystal violet (CV) was calculated to be 3.677×107. This novel Ag/AAO substrate with substantial “hot spots” exhibited high SERS sensitivity which could obtain extremely low limits of detection (LOD) of 10−12M for CV. Importantly, this SERS platform was employed to detect goat serum and reached a LOD at 1 ng/μl. As a nondestructive detection technique, our SERS-based methodology required small sample quantity which expected to achieve more biomolecular detection.
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Optical properties of Ag@cicada wing substrate deposited by Ag nanoparticles
Wang Mingli,Yan Xiaoya,Shi Guochao,Shang Zubin,Zhang Anqi,Ma Wanli 한국물리학회 2020 Current Applied Physics Vol.20 No.11
In this paper, Ag nanoparticles were deposited on Ag@cicada wing array by using the cicada wings as templates to study its optical properties, including surface enhanced Raman scattering (SERS), polarization and surface enhanced fluorescence (SEF). The nanogaps between adjacent conical protrusion can be well dominated by adjusting the sputtering time and the optimal substrate AgNPs@Ag@cicada wing arrays have a noteworthy enhancement of SERS signal. Characterization of the prepared optimal substrate certified that it possesses the excellent SERS performances. Basically consistent SERS signal strength at the different polarization angles of the optimal substrate indicates that its polarization-independence. The SEF spectra shows that the optimal substrate has a slightly lower and unstable enhancement at this initial stage of repeated examination due to the weak adhesion between the Ag@cicada wing arrays and Ag nanoparticles. The outstanding optical properties indicate that it has enormous potential in the label-free detection and biological analytes determination.
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Lu Zhu,Jianxin Shi,Guochao Zhao,Dabing Zhang,Wanqi Liang 한국식물학회 2013 Journal of Plant Biology Vol.56 No.1
The tapetum of the anther locule encloses the malereproductive cells and plays a supportive role for normalpollen development. However, the underlying mechanismremains less understood. Previously, we identified a completerecessive male sterile mutant, post-meiotic deficient anther1(pda1), with abnormal postmeiotic tapetal development. Inthis study we comprehensively characterized pda1. Chemicalanalysis uncovered that pda1 anther had significant lowerlevels of cutin monomers and cuticular waxes. PDA1 geneencodes an ATP-binding cassette (ABC) half-transporter,namely OsABCG15, which is conserved from algae tohigher plants. In situ RNA hybridization assay showed thatPDA1 is strongly expressed in tapetal cells, and weakly inmicrospores during the anther development. Additionally,the expression of two pollen exine biosynthetic genesCYP704B2 and CYP703A3 was dramatically reduced inpda1 mutant anthers. Altogether, these observations suggestthat the tapetum-expressed ABC transporter PDA1 plays acrucial role in secreting lipidic precursors from the tapetumto developing microspores and the anther epidermis.
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