Molecular cloning, characterization, and heterologous expression analysis of heat shock protein genes (hsp70 and hsp90) of the invasive alien weed, Ageratina adenophora (Asteraceae)

Gong, Wei Na,Xie, Bing Yan,Wan, Fang Hao,Guo, Jian Ying The Korean Society of Weed Science and The Turfgra 2010 Weed Biology and Management Vol.10 No.2

An invasive alien weed, Ageratina adenophora, has become widespread in the southwest of China due to its high environmental adaptation. In the present study, in order to understand the molecular aspects of resistance to heat and cold stress, we investigated the characterization and expression of heat shock protein genes (designated as Aahsp70 and Aahsp90) in A. adenophora. The cDNA of A. adenophora Aahsp70 and Aahsp90 was cloned by the combination of homology cloning and rapid amplification of cDNA ends (RACE). The Basic Local Alignment Search Tool analyses revealed that Aahsp70 and Aahsp90 shared high levels of identity with corresponding proteins from other species. Hybridization was employed to examine the expression patterns of Aahsp70 and Aahsp90. The relative expression levels of the Aahsp70 and Aahsp90 transcripts were both up-regulated and reached maximal levels at 2-4 h after heat shock. Then, Aahsp70 dropped progressively to the original level at ~4 h after heat shock. The transcriptional changes of Aahsp70 and Aahsp90 both were more obvious under cold stress. Recombinant Aahsp70 and Aahsp90 improved the viability, in comparison with the control cultures, of Escherichia coli under stress conditions. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of the cell lysates suggested that the protective effect in vivo was related to increased thermo-stability of the soluble cytosolic proteins. This study emphasizes that the relationship between, and regulation of, heat shock proteins might incorporate variable strengths to increase the adaptation of A. adenophora.

Trajectory control of tunnel boring machine based on adaptive rectification trajectory planning and multi-cylinders coordinated control

Tong Liu,Guo-fang Gong,Hua-yong Yang,Yu-xi Chen,Yi Zhu 한국정밀공학회 2019 International Journal of Precision Engineering and Vol.20 No.10

Trajectory control of tunnel boring machine (TBM) has important implications for excavation efficiency and tunnel quality. A novel rectification trajectory planning method is proposed for TBM with adaptively designed direction and curvature against different attitude deviations and target path, and is comparatively studied with traditional method via numerical simulations. TBM could be fully-actuated by decoupling thrust and torque cylinders in horizontal and vertical planes, respectively. After the tropology analysis of the 4-SPS/PS structure, the real-time expected motions of torque cylinders in vertical plane are derived via reverse kinematics together with synchronous thrust cylinders; so does cylinders in horizontal plane. The proportional direction valves of torque and gripper cylinders are compensated with fixed pressure drop. A compound displacement tracking controller could be established, including flow-speed feed-forward with dead-band compensation and displacement feedback by fuzzy proportional–integral (PI) controller with separated integration. Synchronous controller of integral separated PI structure is also proposed. Experiment results on a Φ 2.5 m scaled TBM indicate that, the displacement tracking performance of cylinders under the compound controller against unbalanced load, which is ± 0.9 mm for high-speed thrust cylinders and ± 0.13 mm for low-speed gripper and torque cylinders, could meet the tolerance of trajectory planning and realize accurate attitude correction.

Sliding Mode Control with Adaptive Fuzzy Immune Feedback Reaching Law

Chenchen Sun,Guo-fang Gong,Hua-yong Yang 제어·로봇·시스템학회 2020 International Journal of Control, Automation, and Vol.18 No.2

In this paper, a novel adaptive fuzzy immune feedback reaching law (AFIFRL) based sliding mode control (SMC) strategy is proposed for uncertain nonlinear systems with time-varying disturbances. First, a nonlinear immune feedback reaching law (IFRL) inspired by biological immune feedback regulation mechanism is designed to alleviate chattering effect without losing the robustness against disturbances. Second, an improved IFRL is developed in a thin boundary layer to enhance tracking performance. Then, the applied fuzzy controller adjusts the boundary layer online to further improve control performance despite large system uncertainties and disturbances. Furthermore, an adaptive law is employed to estimate the unknown bound of uncertainties, which can effectively attenuate chattering and minimize control effort. The stability analysis is derived by Lyapunov stability theorem. Finally, numerical simulations are conducted to evidence the effectiveness and superiority of the proposed AFIFRL based SMC scheme.

Inhibition of Citrate Synthase Thermal Aggregation In Vitro by Recombinant Small Heat Shock Proteins

Wei Na Gong,Ming Yue,Bing Yan Xie,Fang Hao Wan,Jian Ying Guo 한국미생물 · 생명공학회 2009 Journal of microbiology and biotechnology Vol.19 No.12

Identification of seven novel virulence genes from Xanthomonas citri subsp. citri by Tn5-based random mutagenesis

Xue Song,Jing Guo,Wen-xiu Ma,Zhi-yuan Ji,Li-fang Zou,Gong-you Chen,Hua-song Zou 한국미생물학회 2015 The journal of microbiology Vol.53 No.5

To identify novel virulence genes, a mutant library of Xanthomonas citri subsp. citri 29-1 was produced using EZ-Tn5 transposon and the mutants were inoculated into susceptible grapefruit. Forty mutants with altered virulence phenotypes were identified. Nine of the mutants showed a complete loss of citrus canker induction, and the other 31 mutants resulted in attenuated canker symptoms. Southern blot analysis revealed that each of the mutants carried a single copy of Tn5. The flanking sequence was identified by plasmid rescue and 18 different ORFs were identified in the genome sequence. Of these 18 ORFs, seven had not been previously associated with the virulence of X. citri subsp. citri and were therefore confirmed by complementation analysis. Real-time PCR analysis showed that the seven genes were upregulated when the bacteria were grown in citrus plants, suggesting that the expression of these genes was essential for canker development.

Mll3 Genetic Variants Affect Risk of Gastric Cancer in the Chinese Han Population

Li, Bing,Liu, Hong-Yi,Guo, Shao-Hua,Sun, Peng,Gong, Fang-Ming,Jia, Bao-Qing Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.7

It is reported that the expression level of MLL3 in gastric cancer tissue highly correlates with tumor progression. However, whether MLL3 genetic variants are associated with the risk of gastric cancer remains unclear. In this study, we conducted a genotyping analysis for MLL3 in 314 cases of gastric cancer and 322 controls from the Chinese Han population. 4 SNPs (rs6943984, rs4725443, rs3800836, rs6464211) were selected for the present analysis. We found 2 SNPs (rs6943984, rs4725443) of MLL3 gene were significantly associated with the risk of gastric cancer : the rs6943984 with the minor allele A and rs4725443 with the minor allele C revealed strong associations with increased gastric cancer risk [P < 0.001, OR=1.97, 95% CI=1.48~2.64 and P <0.001, OR=2.23, 95% CI=1.54~3.24]. Haplotype analysis of the four SNPs showed that haplotype A-T-A-C, G-T-G-C, and G-C-A-C increased the risk of gastric cancer (P <0.001, P=0.18, and P<0.001, respectively), while haplotype G-T-A-C significantly reduced the risk of gastric cancer (P <0.001). We concluded that MLL3 variants are significantly associated with gastric cancer risk. Our results for the first time provided new insight into susceptibility factors of MLL3 gene variants in carcinogenesis of gastric cancer of the Chinese Han population.

MicroRNA-328 Inhibits Proliferation of Human Melanoma Cells by Targeting TGFB2

Li, Jing-Rong,Wang, Jian-Qin,Gong, Qing,Fang, Rui-Hua,Guo, Yun-Long Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.4

Some microRNAs (miRNAs) have been shown to act as oncogenes or tumor suppressor genes in human melanomas. miR-328 is upregulated in blood cells of melanoma patients compared to in healthy controls. This suggests a role for miR-328 in melanoma that warrants investigation. In this study, we demonstrated miR-328 levels to be dramatically decreased in human melanoma cell lines. Moreover, forced expression of miR-328 inhibited proliferation and induced G1-phase arrest of the SK-MEL-1 melanoma cell line. We identified TGFB2 as a direct target gene for miR-328 using a fluorescent reporter assay and western blotting. Levels of TGFB2 were dramatically increased in human melanoma cell lines and were inversely correlated with the miR-328 expression level. Our findings provide new insights into the mechanisms of human melanoma development, indicating that miR-328 has therapeutic potential for this disease.