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- 저자 : Guo Junyun (검색결과 2 건)
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Wang Haibo,Guo Junyun 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.1
The abscisic acid (ABA) plays a crucial role not only in plant development but also in adaptive responses to biotic and abiotic stresses. 9-cis-epoxycarotenoid dioxygenase (NCED), a key rate-limiting enzyme in ABA biosynthesis in plants, is involved in the response of plants to stress. To better understand the roles of ABA and its interactions during cold stress in Jatropha curcas, the JcNCED3 gene was cloned from J. curcas, and its overexpression was functionally verified in yeast and Arabidopsis thaliana. The full-length cDNA of JcNCED3 was 2450 bp and contained an entire open reading frame (ORF) of 1770 bp that encodes a polypeptide of 589 amino acids with a theoretical molecular weight of 65.45 kDa and pI value of 6.97. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis revealed that JcNCED3 was specifically expressed in different organs, abundantly in leaves and cotyledons, but scarcely in stems. The expression of the JcNCED3 gene was remarkably cold-induced in stems and roots, reaching its highest levels after 3 h (1.23-fold) of chilling stress in stems. Interestingly, overexpression of JcNCED3 in yeast could notably enhance the cold resistance of the INVSc1 host strain. Furthermore, transgenic Arabidopsis also showed stronger resistance to cold than the control. The results of this study might provide a theoretical foundation for further studies on the putative application potential of JcNCED3-related genetic engineering in J. curcas.
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Fu Shangfeng,Zhang Hongyan,Li Xiancai,Zhang Qiling,Guo Chunyan,Qiu Keqing,Feng Junyun,Liu Xiaoxiao,Liu Dewu 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.2
BACKGROUND: Diabetic wound healing remains a major challenge due to the impaired functionality of angiogenesis by persistent hyperglycemia. Mesenchymal stem cell exosomes are appropriate candidates for regulating the formation of angiogenesis in tissue repair and regeneration. Here, we explored the effects of exosomes derived from human amniotic mesenchymal stem cell (hAMSC-Exos) on the biological activities of human umbilical vein endothelial cells (HUVECs) treated with high glucose and on diabetic wound healing and investigate lncRNAs related to angiogenesis in hAMSC-Exos. METHODS: hAMSCs and hAMSC-Exos were isolated and identified by flow cytometry or western blot. A series of functional assays such as cell counting kit-8, scratching, transwell and tube formation assays were performed to evaluate the potential effect of hAMSC-Exos on high glucose-treated HUVECs. The effect of hAMSC-Exos on diabetic wound healing were tested by measuring wound closure rates and immunohistochemical staining of CD31. Subsequently, the lncRNAs profiles in hAMSC-Exos and hAMSCs were examined to screen the lncRNAs related to angiogenesis. RESULTS: The isolated hAMSC-Exos had a size range of 30–150 nm and were positive for CD9, CD63 and CD81. The hAMSC-Exos facilitate the functional properties of high glucose-treated HUVECs including the proliferation, migration and the angiogenic activities as well as wound closure and angiogenesis in diabetic wound. hAMSC-Exos were enriched lncRNAs that related to angiogenesis, including PANTR1, H19, OIP5-AS1 and NR2F1-AS1. CONCLUSION: Our findings demonstrated hAMSC-Exos facilitate diabetic wound healing by angiogenesis and contain several exosomal lncRNAs related to angiogenesis, which may represent a promising strategy for diabetic wound healing.
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