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RISS 인기검색어
- 검색키워드
- 저자 : Gulshan Mohammed (검색결과 3 건)
- 무료
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Duration of Preservation Affect the Quality of Chilled Black Bengal Buck Semen
Md Gulshan Anowar Pradhan,Md Saidur Rahman,Woo-Sung Kwon,Dipendra Mishra,Md Mostofa Kamal,Mohammad Musharraf Uddin Bhuiyan,Mohammed Shamsuddin 韓國受精卵移植學會 2013 한국동물생명공학회지 Vol.28 No.2
The study focuses on the quality assessment of Black Bengal buck semen preserved at chilled condition. In this in vitro trial, collected semen from Black Bengal bucks was preserved at chilling temperature (4▲5줛) in tris-glucosecitrate yolk medium of 1:5 ratios for four days. Artificial Vagina (AV) method was utilized to collect semen from buck. General evaluation of semen includes the color, mass activity and density were measured by direct visual examination. However, computer-assisted sperm analysis (CASA) and phase contrast microscopy were used to figure out the motility (%), hyper-activated (HYP) motility (%) and number of abnormal spermatozoa (%) initially, and at every 24 h intervals. The result revealed that spermatozoa preserved at chilling temperature showed significantly (P<0.05) lower motility and HYP motility with the progression of preservation. The number of phenotypically abnormal spermatozoa significantly (P<0.05) increased following preservation. Although significant positive correlation (r=0.945; P<0.05) was existed between % motile and % HYP motile spermatozoa however, the % of morphologically abnormal spermatozoa was negatively correlated with % motile (r=긏0.997; P<0.05) and % HYP motile spermatozoa (r=긏0.946; P<0.01). Therefore, we concluded that the quality of chilled semen progressively losses its viability and doesn…t remain useable after certain period of preservation with respect to its motility and morphology.
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Duration of Preservation Affect the Quality of Chilled Black Bengal Buck Semen
Pradhan, Md. Gulshan Anowar,Rahman, Md. Saidur,Kwon, Woo-Sung,Mishra, Dipendra,Kamal, Md. Mostofa,Bhuiyan, Mohammad Musharraf Uddin,Shamsuddin, Mohammed The Korean Society of Embryo Transfer 2013 한국동물생명공학회지 Vol.28 No.2
The study focuses on the quality assessment of Black Bengal buck semen preserved at chilled condition. In this in vitro trial, collected semen from Black Bengal bucks was preserved at chilling temperature ($4{\sim}5^{\circ}C$) in tris-glucosecitrate yolk medium of 1:5 ratios for four days. Artificial Vagina (AV) method was utilized to collect semen from buck. General evaluation of semen includes the color, mass activity and density were measured by direct visual examination. However, computer-assisted sperm analysis (CASA) and phase contrast microscopy were used to figure out the motility (%), hyper-activated (HYP) motility (%) and number of abnormal spermatozoa (%) initially, and at every 24 h intervals. The result revealed that spermatozoa preserved at chilling temperature showed significantly (P<0.05) lower motility and HYP motility with the progression of preservation. The number of phenotypically abnormal spermatozoa significantly (P<0.05) increased following preservation. Although significant positive correlation (r=0.945; P<0.05) was existed between % motile and % HYP motile spermatozoa however, the % of morphologically abnormal spermatozoa was negatively correlated with % motile (r=-0.997; P<0.05) and % HYP motile spermatozoa (r=-0.946; P<0.01). Therefore, we concluded that the quality of chilled semen progressively losses its viability and doesn't remain useable after certain period of preservation with respect to its motility and morphology.
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Naga Thirumalesh Chevala,Suryaprabha Matangi,Naga Pavan Kumar Chevala,Gulshan Mohammed,Sarvan Mani Kiran Seethamraju,Rama Rao Nadendla 한국약제학회 2015 Journal of Pharmaceutical Investigation Vol.45 No.6
The current research concerns the development of the formulation of fluoxetine HCl (anti-depressant) oro flashes the new prospective by using solvent casting method. Oro flashes are quick dissolving oral films and the materials used were hydrophilic. Fluoxetine HCl formulations were developed since oro flashes improves bioavailability to attain onset of action, along with the patient compliance. Fluoxetine HCl is used to treat major depressive disorder, bulimia nervosa (an eating disorder), obsessive–compulsive disorder, panic disorder, and premenstrual dysphoric disorder. Polyvinyl alcohol (PVA) undergoes polymerization and forms oro flash film. Five different formulations were developed by using different polymeric concentrations of PVA. Polyvinyl pyrollidone K30 (PVP K30) used as super disintegrating agent and plasticizer, whereas menthol and mannitol were used as sweetener, flavoring agent respectively and water as a solvent system. In the study five formulations (F1, F2, F3, F4, and F5) were evaluated for the properties such as thickness, folding endurance, drug content uniformity, surface pH, in vitro disintegration time, dissolution studies, % moisture loss, % moisture uptake, weight variation test, conductivity test. Results revealed that, F3 formulation can be considered as best, which has morphological features are homogenous and transparent with optimum thickness 0.23 ± 0.02 lm, folding endurance (>250 times number of folds on the same crease), drug content uniformity (λmax at 225 nm) 97.60 ± 1.28, surface pH 6.654 ± 0.020, in vitro disintegration time 11 ± 1.20 s, in vitro dissolution studies 97.30 ± 0.75 %, % moisture loss 5.021 ± 0.052 %, % moisture uptake 3.634 ± 0.102 %, weight variation test 48.74 ± 1.0, conductivity test 61 μS which has maximum drug release (97.3 %) which facilitates maximum bioavailability.
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