The Effect of Building Characteristics and Psychological Variables to Occupants' IEQ Satisfaction in Office Buildings

Heejin Han,Chungyoon Chun,Yoorim Choi,John Goins,Hui Zhang,Edward Arens 한국생활환경학회 2013 한국생활환경학회지 Vol.20 No.6

The purpose of this study is to find building and psychological variables which affect occupants" IEQ (Indoor environment quality) satisfaction in office buildings. The results showed that (1) occupants" IEQ satisfaction increased with behavior adjustability, satisfaction with interior design, and level of ensuring privacy. (2) Occupants" satisfaction with each physical condition (thermal, noise, lighting, indoor air quality) decreased when building scale is too large. Building scale also had a significant effect on behavior adjustability. (3) The linear relationship between all psychological variables and IEQ satisfaction was proved. (4) As IEQ satisfaction (thermal, noise, lighting, indoor air quality) increased, overall workspace satisfaction did also increase. Especially, among four IEQ satisfactions, noise satisfaction was the most effective factor on overall workspace satisfaction.

Effective Treatment of an Orthotopic Xenograft Model of Human Glioblastoma Using an EGFR-retargeted Oncolytic Herpes Simplex Virus

Uchida, Hiroaki,Marzulli, Marco,Nakano, Kenji,Goins, William F,Chan, Janet,Hong, Chang-Sook,Mazzacurati, Lucia,Yoo, Ji Young,Haseley, Amy,Nakashima, Hiroshi,Baek, Hyunjung,Kwon, Heechung,Kumagai, Izum Elsevier 2013 MOLECULAR THERAPY Vol.21 No.3

Gene therapy: Targeting HSV Infection to gD Receptor-negative Cells Using a Soluble Receptor

권희중 ( Hee Chung Kwon ),( Qing Bai ),( Kelly Felmet ),( William F. Goins ),( Justis F. Cohen ),( Joseph C. Glorioso ) 대한간암연구회 2004 대한간암연구회 학술심포지엄 Vol.7 No.-

Soluble V Domain of Nectin-1/HveC Enables Entry of Herpes Simplex Virus Type 1 (HSV-1) into HSV-Resistant Cells by Binding to Viral Glycoprotein D

Kwon, Heechung,Bai, Qing,Baek, Hyun-Jung,Felmet, Kelly,Burton, Edward A.,Goins, William F.,Cohen, Justus B.,Glorioso, Joseph C. American Society for Microbiology 2006 Journal of virology Vol.80 No.1

<B>ABSTRACT</B><P>Interaction of herpes simplex virus (HSV) glycoprotein D (gD) with specific cellular receptors is essential for HSV infection of susceptible cells. Virus mutants that lack gD can bind to the cell surface (attachment) but do not enter, implying that interaction of gD with its receptor(s) initiates the postattachment (entry) phase of HSV infection. In this report, we have studied HSV entry in the presence of the gD-binding variable (V) domain of the common gD receptor nectin-1/HveC to determine whether cell association of the gD receptor is required for HSV infection. In the presence of increasing amounts of the soluble nectin-1 V domain (sNec1123), increasing viral entry into HSV-resistant CHO-K1 cells was observed. At a multiplicity of 3 in the presence of optimal amounts of sNec1123, approximately 90% of the cells were infected. The soluble V domain of nectin-2, a strain-specific HSV entry receptor, promoted entry of the HSV type 1 (HSV-1) Rid-1 mutant strain, but not of wild-type HSV-1. Preincubation and immunofluorescence studies indicated that free or gD-bound sNec1123 did not associate with the cell surface. sNec1123-mediated entry was highly impaired by interference with the cell-binding activities of viral glycoproteins B and C. While gD has at least two functions, virus attachment to the cell and initiation of the virus entry process, our results demonstrate that the attachment function of gD is dispensable for entry provided that other means of attachment are available, such as gB and gC binding to cell surface glycosaminoglycans.</P>

Herpes Simplex Virus Targeting to the EGF Receptor by a gD-Specific Soluble Bridging Molecule

Nakano, Kenji,Asano, Ryutaro,Tsumoto, Kouhei,Kwon, Heechung,Goins, William F.,Kumagai, Izumi,Cohen, Justus B.,Glorioso, Joseph C. Elsevier 2005 MOLECULAR THERAPY Vol.11 No.4

<P><B>Abstract</B></P><P>Herpes simplex virus 1 (HSV-1) enters cells via initial binding of envelope glycoproteins (g) C and B to cell-surface glycosaminoglycans (GAGs) and subsequent membrane fusion involving envelope gD, gB, and gH/gL. Current insights suggest that the fusion process is initiated by interaction of gD with a cognate cellular receptor, such as the widely distributed cell adhesion molecule nectin-1. To redirect the tropism of HSV-1, we have generated a soluble adapter protein (P-V528LH) comprising the gD-binding variable domain of nectin-1 fused to a single-chain antibody (528LH) recognizing the EGF receptor. The adapter molecule enabled HSV-1 entry into naturally nonpermissive CHO cells expressing the human EGF receptor, but not into CHO cells lacking the receptor, and entry was not observed when the antibody portion of the adapter was replaced with an antibody of different specificity. Adapter-mediated entry increased with the viral dose and was nearly as efficient as direct viral entry into nectin-1-bearing CHO cells. Entry depended on viral gD and was diminished in the absence of cellular GAGs. These experiments represent the first demonstration that a soluble molecule can direct HSV infection via a new receptor, supporting the possible utility of this approach for HSV retargeting.</P>

스마트온실내 작물생산을 위한 인공광 재배시스템 연구

허정욱 ( Jeong-wook Heo ),김현환 ( Hyun-hwan Kim ),정충렬 ( Chung-ryeol Jeong ),이공인 ( Goin-in Lee ) 한국환경농학회 2016 한국환경농학회 학술대회집 Vol.2016 No.-

스마트온실(식물공장)에서는 태양광 대신 형광등, LED(Light Emitting Diode, 발광다이오드)와 같은인공광을 사용하여 병충해 방제를 위한 농약과 같은 화학물질을 사용하지 않고 작물을 안전하게 재배할수 있으며, 작물의 종류에 따라 다양한 인공광원과 재배시스템을 이용할 수 있다. 스마트온실은 토경재배가 아닌 수경재배방식을 주로 채택하고 있으며, 온도 및 습도 등의 환경 모니터링 및 제어시스템을 활용하여 작물 생장에 적합한 환경을 제공한다. 현재, 이와 같은 스마트온실을 활용하여 상추와 같은 호냉성 엽채류를 중심으로 한 재배시스템 개발 및 재배기술 관련 연구가 활발히 진행되고 있으며 실용화 중에 있다. 그러나 수경재배 조건에서 감초, 당귀 및 방풍 등 뿌리부위를 식용으로 하는 작물을 재배할 경우 기존 수경재배시스템에서는 재배기간이 경과함에 따라 뿌리 엉킴과 부패 현상이 발생하여 장기재배가 곤란하여 신선엽과 뿌리재배를 겸용한 재배시스템에 관한 시스템 연구가 필요하다. 따라서 본 연구에서는 스마트온실내 연중 친환경 작물의 신선엽과 뿌리를 생산하기 위한 인공광형 재배시스템을 개발하고 방풍 재배시험을 통하여 시스템 구동시험을 수행하였다. 재배시스템은 배양액 수조를 중심으로 좌, 우 양측 이동형 인공광원부(60×30 cm), 재배부(80×60 cm) 및 근권부(80×14×30 cm)로 구성하였으며, 인공광원은 LED와 형광등 혼합의 측광조사가 가능하도록 설계하여 작물 지상부 상부에 부착하였다. 뿌리생장을 위한 근권부는 분무경, 담액경과 배지경이 가능하도록 구성하였다. 또한, 지하 근권부에 용존산소농도를 제어할 수 있도록 산소제어장치를 부착하였으며, 근권부 배양액 수조는 일반 담액경, 분무경과 배지경이 가능한 구조로 설계하였다. 구동시험을 위한 식물 공시재료는 신선엽과 뿌리를 가용하는 방풍(Ledebouriella seseloides L.) 실생묘로 하였다. 재배시험 기간 동안 광강도 및 1일 광조사시간은 각각 150 μmol m-2 s-1 및 12 시간이였으며, 재배장치가 놓인 스마트온실내 온도 및 습도는 각각 20±1°C 50±10%로 제어하였다. 배지경 구동시험을 위해 목탄, 하이드로볼 및 난석 등 3종의 배지를 선정하고 세척한 후 배양액 수조에 채웠다. 담액경과 재배시스템내 배지경 재배효과를 비교하기 위하여 수경재배 정식판에 본엽이 2~3매 전개된 실생묘를 정식한 후 5주째에 지상부 생체중, 엽수 등의 지상부 생육을 조사하였으며 재배시험은 5주 간격으로 3회 반복하였다. 일반 담액경 시스템에서 재배한 방풍 지상부 생체중은 배지경에 비해 유의하게 증가하였으며, 배지 종류에 따른 생체중 변화는 없었다. 담액경 시스템에서 방풍잎의 생체중은 배지경 대비 약 1.5배 증가하였으나, 하이드로볼 배지경에 비해 잎의 건물중은 수경재배방식에 따른 유의성은 없는 것으로 나타났다. 엽내 엽록소 함량을 조사한 결과, 수경재배방식 및 배지종류의 차이에 따른 영향은 없었으며 배지경에 있어서 실생묘 개체당 엽수는 하이드로볼 대비 난석 처리구에서 증가하였다. 잎수확을 3회 반복하여 지상부 생장을 조사한 결과, 생체중 이외에 수경재배방식 및 배지종류의 차이와 상관없이 인공광 재배시스템내에서 방풍을 재배할 경우 방풍의 뿌리 및 지상부 잎생장에 문제가 발생하지 않는 것으로 보아, 스마트온실내 뿌리가용 작물의 안정생산이 기대된다. 앞으로 지상부 잎의 수확량 및 뿌리 생장이나 비대를 조사하고 비대생장 촉진을 위한 산소공급 시험을 수행, 재배시스템을 활용한 적정 수경재배조건을 결정할 예정이다.

04 포스터 발표 : 자연생태 환경 분야(PN) ; PN-01 : 식물공장 시스템내 광강도 및 배양액 농도 제어가 뿌리부추 및 고려엉겅퀴 생장에 미치는 영향

허정욱 ( Jeong Wook Heo ),김현환 ( Hyun Hwan Kim ),이광재 ( Kwang Jae Lee ),신현만 ( Hyun Man Shin ),이공인 ( Goin In Lee ),최규홍 ( Kyu Hong Choi ) 한국환경농학회 2015 한국환경농학회 학술대회집 Vol.2015 No.-

Culture-technic researches for year round production of agricultural plants inside plant factory system, which controlled the environmental factors of air temperature, relative humidity, nutrient, CO2 concentration, light intensity or quality, have been vigorously proceeded. In this study, control effects of light intensity and nutrient-solution concentration were investigated on growth of perennial chive (Allium hookeri) seedstock and Korean thistle (Cirsium setidens) seedlings inside plant factory system. Yamazaki solution was irrigated by deep flow technique (DFT) system during the culture period. Air temperature and relative humidity inside the system were maintained at 22℃ and 50%, respectively. Korean thistle seedlings which developed two true leaves were cultured under 1.5, 2.0 or 2.5 dS m-1 EC (Electrical conductivity), 5.8 pH, and 150 μmol m-2 s-1 PPF for 8 weeks. Fluorescent lamps with 50, 100 or 150 μmol m-2 s-1 photosynthetic photon flux (PPF) were used for hydroponic culture (EC 1.5 dS m-1, pH 5.8) of chive seedstocks for 7 weeks. Fresh weight and shoot length emerged from chive seedstocks grown under 150 μmol m-2 s-1 PPF were 76 and 24% increased compared to 50 μmol m-2 s-1 PPF, respectively. Number of shoots in chive was also greater in 150 μmol m-2 s-1 PPF, and significantly reduced under 100 μmol m-2 s-1 PPF. When EC was maintained at 1.5 or 2.0 dS m-1 for 56 days, increasing in leaf fresh and dry weight of Korean thistle were remarkable. Fresh and dry weights were 5.8 and 3.5 times greater in 1.5 or 2.0 dS m-1 EC than in 2.5 dS m-1 EC treatment, respectively. Number of unfolded leaves increased to 96% under 1.5 or 2.0 dS m-1 EC. The chive seedstock and Korean thistle were successfully cultured under the DFT system using fluorescent lamps. From the above results, it is suggested that shoot growth such as a fresh weight, dry weight, or shoot elongation could be significantly stimulated by the light intensity and EC tested in this experiment. The above results show production possibility of perennial plants besides leaf vegetables, and studies on change in nutrient elements inside the plants cultured under the system are necessary.