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Escherichia coli O157:H7 Intimin의 Expression 및 C-terminal 부위의 특성
손원근,Gannon, V. P. J. 한국수의공중보건학회 2001 예방수의학회지 Vol.25 No.3
Portions of the intimin genes of Escherichia coli O157:H7 strain 319 and of the enteropathogenic E. coli O127:H6 strain E2348/69 were amplified by PCR and cloned into pET-28a (+) expression vectors. The entire 934 aa of E. coli O157:H7 intimin, the C-terminal 306 aa of E. coli O157:H7 intimin, and the C-terminal 311 aa of E. coli O127:H6 intimin were expressed as protein fusions with a six histidine residue tag (His-tag) in pET-28a (+). Rabbit antisera raised against the six His-tag 3' end one-third portion of E. coli O157:H7 intimin protein fusion reacted strongly in Western blots with original antigen and its homology protein, the six His-tag-fusioned protein containing full-length intimin of E. coli O157:H7, while reacting weakly with the his-fusioned intimin from enteropathogenic E. coli serotype O127:H6. In contrast with the antiseraraised against the sis His-tag 3'end portion of E. coli O127:H6 recognized only the original antigen, His-intO127C.
Unexpected effects of ivermectin and selamectin on inducible CreER activity in mice
Peter A. Kropp,Gabrielle V Rushing,Asa A. Brockman,Erin N. Z. Yu,Rebecca A. Ihrie,Maureen Gannon 한국실험동물학회 2020 Laboratory Animal Research Vol.36 No.4
Background: Anti-parasitics are frequently used in research animal facilities to treat a multitude of common infections, with pinworms and fur mites being amongst the most common. Ivermectin and selamectin are common oral and topical treatments for these infections, respectively. Although commonly thought to be innocuous to both the research animals and any transgenic elements that the animals may carry, evidence exists that ivermectin is capable of activating the recombinase activity of at least one CreER. The goal of the current study was to determine if there was an effect of either anti-parasitic agent on the activity of CreER proteins in transgenic mice. Case presentation: We analyzed the offspring of transgenic mice exposed to either ivermectin or selamectin during pregnancy and nursing. Through analysis of reporter genes co-expressed with multiple, independently generated transgenic CreER drivers, we report here that ivermectin and selamectin both alter recombinase activity and thus may have unintended consequences on gene inactivation studies in mice. Conclusions: Although the mechanisms by which ivermectin and selamectin affect CreER activity in the offspring of treated dams remain unclear, the implications are important nonetheless. Treatment of pregnant transgenic mice with these anti-parasitics has the potential to alter transgene activity in the offspring. Special considerations should be made when planning treatment of transgenic mice with either of these pharmacologics.
Jennifer Palacio,Daisy Sanchez,Shenae Samuels,Bar Y. Ainuz,Raelynn M. Vigue,Waleem E. Hernandez,Christopher J. Gannon,Omar H. Llaguna 한국간담췌외과학회 2023 Annals of hepato-biliary-pancreatic surgery Vol.27 No.3
Backgrounds/Aims: Current literature presents limited data regarding outcomes following conversion at the time of minimally invasive pancreaticoduodenectomy (MI-PD). Methods: The National Cancer Database was queried for patients who underwent pancreaticoduodenectomy. Patients were stratified into three groups: MI-PD, converted to open pancreaticoduodenectomy (CO-PD), and open pancreaticoduodenectomy (O-PD). Multivariable modeling was applied to compare outcomes of MI-PD and CO-PD to those of O-PD. Results: Of 17,570 patients identified, 12.5%, 4.2%, and 83.4% underwent MI-PD, CO-PD, and O-PD, respectively. Robotic pancreaticoduodenectomy (R-PD) resulted in a higher lymph node yield (n = 23.2 ± 12.2) even when requiring conversion (n = 22.4 ± 13.2, p < 0.001). Margin positivity was higher in the CO-PD group (26.6%) than in the MI-PD group (21.3%) and the O-PD (22.6%) group (p = 0.017). Length of stay was shorter in the MI-PD group (laparoscopic pancreaticoduodenectomy 10.4 ± 8.6, R-PD 10.6 ± 8.8) and the robotic converted to open group (10.7 ± 6.4) than in the laparoscopic converted to open group (11.2 ± 9) and the O-PD group (11.5 ± 8.9) (p < 0.001). After adjusting for patient and tumor characteristics, both MI-PD (odds ratio = 1.40; p < 0.001) and CO-PD (odds ratio = 1.24; p = 0.020) were significantly associated with an increased likelihood of long-term survival. Conclusions: CO-PD does not negatively impact perioperative or oncologic outcomes.