Improving Fermentation and Nutritive Quality of Napiergrass Silage by Mixing with Phasey Bean

Yunus, M.,Ohba, N.,Tobisa, M.,Nakano, Y.,Shimojo, M.,Furuse, M.,Masuda, Y. Asian Australasian Association of Animal Productio 2001 Animal Bioscience Vol.14 No.7

To improve the low content of crude protein and low quality fermentation of napiergrass silage, ensiling with phasey bean was examined. Napiergrasses, (Pennisetum purpureum cv. Merkeron), hybrid napiergrass (Pennisetum purpureum${\times}$ Pennisetum typhoides) and phasey bean (Macroptilium lathyroides cv. Murray) were cultivated in Fukuoka, Japan. The first growth of napiergrasses and phasey bean were harvested at 90 days of age and chopped into about 1 cm lengths. The mixing levels of phasey bean with napiergrasses were 0, 25, 50 and 75; 700 g of plant materials were ensiled into a laboratory silo (1.0 litre. polyethylene container) and incubated for 30 days. After opening silos, pH, total nitrogen (TN), volatile basic nitrogen (VBN) and organic acids (lactic acid: LA, acetic acid: AA, butyric acid: BA) were determined. The experiment was a factorial design of $2{\times}4$ with 3 replicates. The data were analyzed statistically by two-way analysis of variance. Without inclusion of phasey bean, lower quality was observed for hybrid silage than for Merkeron silage. The inclusion of phasey bean at the rate of 25% increased DM and TN, and decreased pH value, VBN/TN, AA and BA in both Merkeron and hybrid napiergrass silages. The value of pH, TN, VBN/TN, AA and BA were not significantly different among 25, 50 and 75% mixing levels of phasey bean. LA production increased significantly with the increase in phasey bean mixing level in both hybrid and Merkeron silages. At all mixing levels of phasey bean, LA production did not show significant differences between Merkeron and hybrid silages.

FOOD INTAKE AND CROP EMPTYING RATE OF CHICKENS TREATED WITH GUANETHIDINE

Furuse, M.,Choi, Y.H.,Mabayo, R.T.,Sugahara, K.,Okumura, J. Asian Australasian Association of Animal Productio 1996 Animal Bioscience Vol.9 No.6

The effect of guanethidine on feeding behavior was investigated in the chicken. Graded levels of chronically administered guanethidine, an adrenergic neurone blocker, at 0, 25, 50 and 100 mg/kg body weight, decreased body weight gain and food intake in a dose dependent manner. The effect of acute guanethidine administration on crop emptying rate of the chicken was also investigated. The highest level (10 mg i.v./kg body weight) of guanethidine significantly delayed crop emptying compared with the control. These results suggest that the sympathetic nervous system in the chicken is an important factor for the regulation of feeding behavior associated with food passage from the crop.

Effects of Adding Urea and Molasses on Napiergrass Silage Quality

Yunus, M.,Ohba, N.,Shimojo, M.,Furuse, M.,Masuda, Y. Asian Australasian Association of Animal Productio 2000 Animal Bioscience Vol.13 No.11

To standardize proper formulation of urea and molasses, the former to increase crude protein content of tropical grass and the latter for improving its silage quality, we examined the fermentation quality of silage of fresh and wilted napiergrass (Pennisetum purpureum Schumach) with different levels of urea and molasses with or without lactic acid bacteria (LAB). Silage was made of napiergrass with conditions of fresh young (Exp. 1),young wilted for half day (Exp. 2) and fresh mature (Exp. 3). Chopped plant materials of about 1cm length were ensiled into a laboratory silo and incubated for one month at $25^{\circ}C$. The treatments were the combination of 0, 0.2 and 0.6% of urea and 0, 2 and 5% of molasses (fresh material basis) with or without LAB inoculation. After opening the silo, pH, organic acids, volatile basic nitrogen (VBN) and total nitrogen (TN) were determined. Addition of molasses significantly (p<0.01) lowered pH values in three experiments. Though molasses addition increased lactic acid production even at a higher level of urea, pH values at 0 and 2% molasses were significantly increased by urea in fresh and wilted young silages, but in fresh mature silage it occurred only when molasses was not added. VBN/TN at 0.6% urea were decreased significantly by the highest molasses in three experiments. Significant increases in TN by the increasing of urea addition were observed at all levels of molasses in wilted young and fresh mature silages. In conclusion, a combination of 5% molasses and 0.6% urea could improve the nutritive and fermentation qualities of napiergrass silage under young, wilting and mature conditions.

Liver Cancer Working Group Report

Kudo, M.,Han, K. H.,Kokudo, N.,Cheng, A.-L.,Choi, B. I.,Furuse, J.,Izumi, N.,Park, J.-W.,Poon, R. T.,Sakamoto, M. Oxford University Press 2010 Japanese journal of clinical oncology Vol.40 No.suppl1

<P>Hepatocellular carcinoma is a highly prevalent disease in many Asian countries, accounting for 75-80% of victims worldwide. The incidence of hepatocellular carcinoma varies enormously across Asia, but tends to follow the incidences of hepatitis B infection and liver cirrhosis. The incidence and etiology of hepatocellular carcinoma in Japan are different from the rest of Asia, but similar to that in Western countries because hepatitis C infection is the main etiological factor in Japan. Hepatitis B virus vaccination programs are showing great success in reducing hepatitis B virus-related hepatocellular carcinoma. Screening program improves detection of early hepatocellular carcinoma and has some positive impact on survival, but the majority of hepatocellular carcinoma patients in Asia still present with advanced hepatocellular carcinoma. Long-term outcomes following treatment of even early/intermediate or advanced disease are often unsatisfactory because of a lack of effective adjuvant and systemic therapies. Various clinical practice guidelines for hepatocellular carcinoma have been established and are in use. Clinical diagnosis of hepatocellular carcinoma by imaging diagnosis is replacing diagnosis of hepatocellular carcinoma by pathological confirmation. New imaging and treatment techniques are continuously being developed and guidelines should be updated every 3 or 4 years, incorporating new evidence. New molecularly targeted therapies hold great promise. Sorafenib is the first systemic therapy to demonstrate prolonged survival vs. the placebo in patients with advanced hepatocellular carcinoma. Various other new molecularly targeted agents are currently under investigation.</P>

EFFECT OF DIETARY ASPIRIN ON LAYING PERFORMANCES AND EGG YOLK FATTY ACID COMPOSITION IN JAPANESE QUAIL

Murai, A.,Furuse, M.,Okumura, J. Asian Australasian Association of Animal Productio 1994 Animal Bioscience Vol.7 No.2

In avian species, addition of aspirin to the diet was shown to improve the egg production and to elevate the proportion of essential fatty acid contents in several body tissues. This study was conducted to investigate the effect of dietary aspirin on the accumulation of essential fatty acids in egg yolk. Laying Japanese quail at 170 days of age were fed practical diets supplemented with graded levels (0, 0.4 and 0.8%) of aspirin for 2 weeks. There were no significant differences in final body weight and liver weight. Food intake and egg weight on the 0.8% aspirin diet were significantly lower than those on the 0 or 0.4% aspirin diet. In the liver and egg yolk lipids, the 16:0 in birds fed the 0.8% aspirin diet was significantly higher than that in birds fed the aspirin-free diet. However, the proportion of n-6 poly-unsaturated fatty acids was not affected by feeding aspirin diets.

STUDIES ON METHIONINE METABOLISM IN THE RUMEN BACTERIA OF GOATS

Muramatsu, T.,Numa, M.,Ueda, Y.,Furuse, M.,Okumura, J.,Samukawa, K. Asian Australasian Association of Animal Productio 1994 Animal Bioscience Vol.7 No.2

The metabolic fate of methionine in rumen bacteria was studied by intraruminal administration of $^{15}N$ and $1-^{13}C$ labeled methionine in goats. Time course changes in isotopic abundance of amino acids in the rumen bacteria were determined with a computer-controlled gas-chromatograph mass spectrometer. The results from the transition of peak isotopic abundance in amino acids indicated that in rumen bacteria the $^{15}N$ or $^{13}C$ isotope in the methionine molecule was transferred rapidly to into bacteria, methionine administered intraruminally may not be retained as it is, but would be converted quickly to other metabolites in the bacteria.

COMPARISON OF UTILIZATION OF CELLULOSE AND CORN DIETARY FIBER AS AN ENERGY SOURCE IN CHICKS

Muramatsu, T.,Morishita, T.,Furuse, M.,Okumura, J. Asian Australasian Association of Animal Productio 1992 Animal Bioscience Vol.5 No.1

An experiment was conducted to investigate effects of fiber source on growth performance, N and neutral detergent fiber (NDF) digestibility, and utilization of energy in chicks fed an isocaloric low-energy diet from 7 to 21 days of age. Two fiber sources, cellulose and corn dietary fiber (CDF), were included in a diet at 10, 20 and 30% at the expense of kaolin, an inert diluent. The CDF contained 76.5% NDF consisting mainly of hemicellulose. The results showed that growth performance, N and NDF digestibility, dietary DE and ME values, energy deposition, and NE for production in birds fed CDF were inferior to those in birds fed cellulose. It can be concluded, from the present study, that chicks can utilize cellulose more efficiently than CDF up to a level of 30%.

EFFECT OF BACTERIAL INOCULATION ON NEUTRAL DETERGENT FIBRE DIGESTION AND ENERGY AVAILABILITY IN GERM-FREE CHICKENS

Muramatsu, T.,Niwa, N.,Furuse, M.,Okumura, J.,Ohmiya, K. Asian Australasian Association of Animal Productio 1992 Animal Bioscience Vol.5 No.1

The present study was done to examine whether inoculated and established bacteria in the digestive tract of germ-free (GF) chickens affect growth performance, energy availability, nitrogen utilization and neutral detergent fibre (NDF) digestibility of the host bird fed a high-fibre diet. Gnotobiotic (GB) chicks were made from GF birds by co-inoculating with Ruminococcus albus, and Staphylococcus warneri, only the latter of which was established in the chicken gut. No difference was detected among conventional (CV), GF and GB birds in body weight gain, food intake or food efficiency from 7 to 21 d of age. The amount of nitrogen retained was larger in CV than in GF and GB chicks. DE and ME values of the diet and NDF digestibility were higher in CV birds than in GF and GB counterparts. It was concluded, therefore, that the established bacterium S. warneri did not give any beneficial effects on the host bird as judged by growth performance, energy availability, nitrogen utilization, and NDF digestibility.

INTRACEREBROVENTRICULARLY ADMINISTERED PHENYLALANINE AND TYROSINE: EFFECTS ON FEEDING BEHAVIOUR AND NOREPINEPHRINE CONCENTRATIONS OF SPECIFIC BRAIN SITES IN THE CHICKEN

Choi, Y.-H.,Furuse, M.,Okumura, J.,Shimoyama, Y.,Sugahara, K.,Denbow, D.M. Asian Australasian Association of Animal Productio 1996 Animal Bioscience Vol.9 No.3

A study was carried out to investigate the action of central L-pheylalanine (Phe) and L-tyrosine (Tyr) on food intake of the chicken. In the first trial, Phe ($200{\mu}g/10{\mu}l$) or saline was acutely administered into the right lateral ventricle (i.c.v.) of chickens (5 birds per each group). Birds (4 birds per each group) were administered with the i.c.v. Tyr ($200{\mu}g/10{\mu}l$) or saline in the second trial. The brains of the birds were removed for catecholamine assy 30 min postadministration. Catecholamine concentrations were measured at specific sites of the brain (LH: lateral hypothalamus, PVN: paraventricular nucleus, and VMH: ventromedial hypothalamus). No significant effect of amino acids on the concentration of norepinephrine of brain sites investigated was detected. Food intake and rectal body temperature were also monitored for 6 h after central administrations of Phe, Tyr or saline (5 birds per each group). Both Phe and Tyr, up to $1mg/10{\mu}l$, failed to modulate food intake or rectal body temperature.

THE ROLE OF BILE ACIDS ON THE PLASMA LIPIDS IN CHICKS GIVEN DIETS CONTAINING MEDIUM CHAIN TRIACYLGLYCEROL

Mabayo, R.T.,Furuse, M.,Yang, C-P,Okumura, J. Asian Australasian Association of Animal Productio 1994 Animal Bioscience Vol.7 No.4

The effects of the prevention of micelle formation and bile acid reabsorption, by using cholestyramine (CHOLN), a bile acid binding polymer, on the plasma lipid of Single Comb White Leghorn male chicks given diets containing medium chain triacylglycerol (MCT) and long chain triacylglycerol (LCT) were investigated. Corn oil and glyceryl tricaprylate were used as LCT and MCT sources, respectively. Plasma HDL cholesterol was reduced by CHOLN in all treatments. Plasma LDL cholesterol was reduced by CHOLN in chicks given LCT diet but not in MCT diet which could be accounted to the reduced plasma total cholesterol in LCT diet with CHOLN. It is concluded that bile acid binding does not alter the cholesteremic effect of MCT in the plasma of chicks.