http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Fenny Dane (검색결과 3 건)
- 무료
- 기관 내 무료
- 유료
-
Unraveling stress tolerance mechanisms in plants
Fenny Dane 한국작물학회 2007 한국작물학회 학술발표대회 논문집 Vol.2007 No.11
Abiotic stress conditions caused by temperature extremes, water availability, and ion toxicity severely limit plant growth and productivity. The degree of damage depends on the duration and intensity of the stress, developmental stage and plant species. Plants respond to stress through changes in gene expression, which are controlled by multiple signaling pathways. Our research has focused on gene expression analysis during low temperatures in cold-hardy Poncirus trifoliata, used as rootstock for enhancing cold tolerance in Citrus unshiu (Satsuma mandarin) scion in freeze-prone crop production areas. cDNA-Amplified Fragment Length Polymorphism (cDNA-AFLP) and Quantitative Real-Time (RT) PCR were use to compare expression patterns in rootstock and C. unshiu grafted onto P. trifoliata. Genes with the following known functions were identified: signal transduction and regulation of gene expression (such as MAPK3), osmotic stress response, cell skeleton organization, vesicle trafficking and senescence. Differential expression of genes in roots of drought tolerant cucurbit species (Citrullus colocythis) is being studied in response to drought and plant hormones. A complex interplay between abscisic acid, jasmonic acid and salicylic acid signaling pathways regulates gene expression during adaptive responses to abiotic stress in Citrullus species. Functional analysis and detailed characterization of abiotic stress related genes is in progress.
-
Si, Ying,Dane, Fenny,Rashotte, Aaron,Kang, Kwonkyoo,Singh, Narendra K. Oxford University Press 2010 Journal of experimental botany Vol.61 No.6
<P>A full-length drought-responsive gene <I>Ccrboh</I>, encoding the respiratory burst oxidase homologue (rboh), was cloned in <I>Citrullus colocynthis</I>, a very drought-tolerant cucurbit species. The robh protein, also named NADPH oxidase, is conserved in plants and animals, and functions in the production of reactive oxygen species (ROS). The <I>Ccrboh</I> gene accumulated in a tissue-specific pattern when <I>C</I>. <I>colocynthis</I> was treated with PEG, abscisic acid (ABA), salicylic acid (SA), jasmonic acid (JA), or NaCl, while the homologous <I>rboh</I> gene did not show any change in <I>C</I>. <I>lanatus</I> var. <I>lanatus</I>, cultivated watermelon, during drought. Grafting experiments were conducted using <I>C</I>. <I>colocynthis</I> or <I>C</I>. <I>lanatus</I> as the rootstock or scion. Results showed that the rootstock significantly affects gene expression in the scion, and some signals might be transported from the root to the shoot. <I>Ccrboh</I> in <I>C</I>. <I>colocynthis</I> was found to function early during plant development, reaching high mRNA transcript levels 3 d after germination. The subcellular location of Ccrboh was investigated by transient expression of the 35S::Ccrboh::GFP fusion construct in protoplasts. The result confirmed that Ccrboh is a transmembrane protein. Our data suggest that <I>Ccrboh</I> might be functionally important during the acclimation of plants to stress and also in plant development. It holds great promise for improving drought tolerance of other cucurbit species.</P>
-
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
