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Sun Feng,Li Na,Wang Lijia,Feng Huajun,Shen Dongsheng,Wang Meizhen 한국미생물학회 2020 The journal of microbiology Vol.58 No.11
The stabilization of quorum sensing (QS) is vital for bacterial survival in various environments. Although the mechanisms of QS stabilization in certain conditions have been well studied, the impact of environmental factors has received much less attention. In this study, we show that the supplementation of 25 μM iron in competition experiments and 50 μM in evolution experiments to casein growth cultures significantly increased the possibility of population collapse by affecting elastase production. However, the expression of lasI and lasR remained constant regardless of iron concentration and hence this effect was not through interference with the LasIR circuit, which mainly regulates the secretion of elastase in Pseudomonas aeruginosa. However, the expression of rhlR was significantly inhibited by iron treatment, which could affect the production of elastase. Further, based on both reverse transcription quantitative polymerase chain reaction and gene knock-out assays, we show that iron inhibits the transcription of ppyR and enhances the expression of mexT, both of which decrease elastase production and correspondingly interfere with QS stabilization. Our findings show that environmental factors can affect the genes of QS circuits, interfering with QS stabilization. These findings are not only beneficial in understanding the mechanistic effect of iron on QS stabilization, but also demonstrate the complexity of QS stabilization by linking non-QS-related genes with QS traits.
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Energy Dissipation Characteristics Modelling for Hot Extrusion Forming of Aluminum-Alloy Components
Hongcheng Li,Yuanjie Wu,Huajun Cao,Feng Lu,Congbo Li 한국정밀공학회 2022 International Journal of Precision Engineering and Vol.9 No.6
The hot extrusion forming process is widely used to process aluminum-alloy components in both the automobile and aircraft manufacturing industries. Since it involves pushing the material through the die at increased temperature, it is very energy-intensive despite requiring less blank material allowance. During hot extrusion forming, the multi-stage dynamic conversion of electricity, mechanical energy, and hydraulic energy to heat results in high energy dissipation. In order to improve the power and energy conversion efficiency of hot extrusion forming process, it is necessary to identify the energy dissipation characteristics. The transfer and conversion paths of the electrical, mechanical, and hydraulic energy from the motor to the hydraulic cylinder were firstly depicted based on the motion cycle of the extruder. A bond graph-based energy dissipation model was then proposed for dynamically identifying the energy-saving potentials. The energy dissipation model integrated the power bond graph sub-model of energy conversion elements such as motor, pump, hydraulic valve group, and hydraulic cylinder. These power bond graph sub-models were separately developed to find the energy dissipation state equations of energy conversion elements. An experiment was carried out using data obtained from the energy management system to validate the bond graph-based energy dissipation model. The results have shown that the power and energy conversion efficiency of hot extrusion forming is primarily controlled by the parameters such as extrusion velocity and extrusion force. Both the higher extrusion velocity and lower extrusion force will reduce the power and energy conversion efficiency. An optimal combination of extrusion velocity and pressure can achieve the lowest energy consumption per unit product.
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Threshold magnetoresistance in anistropic magnetic 2D transition metal dichalcogenides
Xu, Hongjun,Hsu, Ming-Chien,Fuh, Huei-Ru,Feng, Jiafeng,Han, Xiufeng,Zhao, Yanfeng,Zhang, Duan,Wang, Xinming,Liu, Fang,Liu, Huajun,Cho, Jiung,Choi, Miri,Chun, Byong Sun,Ó,Coileá,in, Cormac The Royal Society of Chemistry 2018 Journal of Materials Chemistry C Vol.6 No.12
<P>Recently many novel magnetoresistance (MR) phenomena have been reported from studies of two dimensional (2D) materials. Here, we report on the exotic transport behavior of VS2. A large negative and quadratic MR of −10% is observed for an in-plane magnetic field B up to 14 T. Remarkably, when the applied field deviates from the in-plane orientation there is a threshold field, Bc, and the MR shows a plateau of near zero MR. When B < Bc, only a single state exists and the transition between quantum spin states is forbidden. Our work sheds new light on the MR of magnetic 2D materials with localized states and may spur further investigations.</P>
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