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- 저자 : Farshid Sefat (검색결과 2 건)
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Chin Fhong Soon,Sargunan A/L Sundra,Nurfarina Zainal,Farshid Sefat,Mohd Khairul Ahmad,Nafarizal Nayan,Kian Sek Tee,Sok Ching Cheong 한국생물공학회 2019 Biotechnology and Bioprocess Engineering Vol.24 No.5
Mixing and dilution are essential procedures in pharmaceutical operation to aliquot two or more components serially to produce less concentrated and well mixed solutions. However, conventional serial dilution method used in laboratory is tedious and utilized large quantity of plasticwares. In this study, a two-tier microdilution device with two inlets and four outlets was designed, simulated, and prototyped to dilute Cytochalasin-B (CB). Using the microdilution device, CB in linear concentration gradients were produced based on one-step dilution method. The different concentrations of CB were applied to treat monolayer and microtissues of ORL-48 cells. The morphological responses, cell viability and cell proliferation of ORL-48 monolayer cells (2D) and microtissues (3D) treated in four different CB concentrations were investigated via phase contrast microscopy, live/dead stainings, and Alamar Blue® assay, respectively. The results showed that 2D ORL-48 cells were morphologically affected but 3D ORL-48 cells stayed viable and proliferative after treated with similar concentrations of CB. The microdilution device enables serial dilutions to produce reagents in linear concentration gradients via a one-step dilution method.
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Azadeh Izadyari Aghmiuni,Saeed Heidari Keshel,Mostafa Rezaei-Tavirani,Farshid Sefat,Arash Khojasteh,Masoud Soleimani,Farzad Pakdel 한국섬유공학회 2022 Fibers and polymers Vol.23 No.12
Tissue engineering provides new approaches to improve skin lesions. However, cell differentiation onto theengineered substrate with the skin-like pattern is the main challenge. Here we have tried to fabricate such the substrate viastudying the change in polymers ratios and molecular weight, and grafting scaffold with silk fibroin (SF) biomaterial. To thisend, chitosan and PEG were mixed at the volume ratios of 25:75, 50:50, and 65:35, and samples were lyophilized by thefreeze-drying method. Based on the result, the ratio of 65:35 indicated better physicomechanical properties than two otherscaffolds. Afterward, Chi/PEG scaffolds were prepared via mixing chitosan/PEG with (65:35) and PEG molecular weights of2000, 4000, 6000, 10000 Da. It was found that the increase of PEG molecular weight (>4000) was led to the reduction intensile strength and elongation of the scaffold network. Hence, PEG4000 was selected as the optimum molecular weight todesign SF-grafted Chi/PEG scaffold. Therefore, Chi/PEG4000-SF scaffold was designed to evaluate the volume ratio of SF(1 %, 3 %, 5 %) and compare data with the decellularized dermis. The results showed Chi/PEG4000-SF(3%) scaffold not onlywas led to the same elongation as Chi/PEG-SF(5%) scaffold but also created the dermis-like modulus. Moreover, Chi/PEGSF(3%) provided higher expression level of keratinocytes (bio-mimetic pattern) than decellularized dermis due to betterphysicomechanical properties. Hence, it seems that engineered scaffolds can be a more suitable option than native tissue (dueto removal of limitations such as donor sites and immunogenicity, and their mechanical properties). This study can providenovel insight into the better design of skin-engineered scaffolds.
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