http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
이경림,Md. Fakruzzaman,Erdan Wang,김성수,하아나,민찬식,공일근 경상대학교 농업생명과학연구원 2014 농업생명과학연구 Vol.48 No.3
Matrix Metalloproteinases (both MMP2 and -9) play a pivotal role of the embryos hatchingand implantation. Therefore, the objective of this study was carried out to investigate theinfluence of MMP2 and MMP9 on embryo development potential and subsequent effect atmolecular level. There was no significant difference of cleavage rate among the groups. Thedevelopment competence of blastocyst was significantly higher (P<0.05) in MMP9 treatment(39.81±16.61) than that to the combined treatment of MMP2 and –9 (23.68±0.27), but there wasno significant difference among the control vs. MMP2 vs. MMP9 (35.05±2.74 vs. 32.71±6.18vs. 39.81±16.61, respectively). On the other hand, the hatching rate of blastocysts wassignificantly lower (P<0.05) in combined group of MMP2 and –9 (12.55±0.09) (Table1). Theexpression level of MMP2 and MMP9 was significantly lower (P<0.05) in the entire treatmentgroups than that in the control group. But the expression of MMP9 was significantly higher(P<0.05) when compared in the entire treatment groups. The relative expression embryonicdevelopmental gene, IFNt expression level significantly lower (P < 0.05) in the MMP9 embryos. The placenta establishment genes, PLAC8 and SSLP1, expression were significantly higher (P <0.05) in the MMP2 embryos compared to other groups. Transcription regulation gene,HNRNPA2B1, was higher (P < 0.05) in the combined group of MMP2+MMP9 than that in theother groups. In conclusion, our results suggest that MMPs to culture medium improves theblastocyst development rate and further impact on target gene expression analysis.
Development of New Vitrification Method for Preimplantation Mouse Embryo
Ha, A-Na,Fakruzzaman, Md.,Lee, Kyeong-Lim,Wang, Erdan,Lee, Jae-Ik,Min, Chan-Sik,Kong, Il-Keun The Korean Society of Embryo Transfer 2013 한국동물생명공학회지 Vol.28 No.2
The purpose of this study was attempted to new methods in mammalian embryos vitrification. This method was affected to increase of the embryo vitrification efficiency and it would be applied to the field of embryo transfer to recipient by modified loading method of embryo into 0.25 ml plastic straw. The frozen mouse embryos were carried out warmed from two different cell stages (8-cell and blastocyst, respectively) by attachment of an embryo in the vitrification straw (aV) method. All groups were cultured in M-16 medium to determine the development and survivability for 24 h, respectively. Results shown that, the survivability of two different groups were significantly different (94.8% vs. 70.9%). Total cell number was not significantly different the non-frozen blastocyst ($99.7{\pm}12.4$) compared to the post-thaw blastocyst ($94.8{\pm}15.1$). From the 8-cell embryo, total cell number of frozen blastocysts were significantly lower than others groups ($74.7{\pm}14.6$, p<0.05). In the case of cell death analysis, the blastocysts from non-frozen and frozen-thawed 8-cell group were not different ($0.0{\pm}0.0$ vs. $1.9{\pm}3.1$, p>0.05). However, the apoptotic nuclei of blastocyst were significantly observed the frozen-thawed group ($5.4{\pm}4.4$) compared to non-frozen group (p<0.05). Therefore, this new method of embryos using in-straw dilution and direct transfer into other species would be more simple procedure of embryo transfer rather than step-wise dilution method and cryopreservation vessels, so we can be applied in animal as well as human embryo cryopreservation in further.
이경림,방재일,하아나,Md. Fakruzzaman,민찬식,공일근 사단법인 한국동물생명공학회 2014 한국동물생명공학회지 Vol.29 No.2
Matrix Metalloproteinases (MMP)-2 and -9 are participated in embryo development, implantation, remodeling ofepithelial cell and ovulation. The objective of this study is to evaluate an impact of MMP2 and MMP9 on embryonicdevelopmental competence as well as gene expression profiles of in vitro-produced bovine embryos. After in vitrofertilization, embryos of all groups were transferred into IVC-2 medium treated with MMP2 and MMP9 to check theoptimum concentration on the basis of embryo development competence and cell numbers. The optimum concentrationsfor MMP2 and 9 were 1,200 ng/ml and 300 ng/ml. The blastocyst development competence was not different among1,200 ng/ml of MMP2 vs. 300 ng/ml of MMP9 vs. combined MMP2 + 9 vs. control groups (41.46 ± 10.66 vs. 37.73± 8.92 vs. 45.11 ± 11.41% vs. 41.59 ± 11.88, respectively). Furthermore, the developmental competences to hatchingand hatched blastocysts were not also different among the same groups (79.84 ± 12.63 vs. 83.3 ± 17.46 vs. 78.55± 14.48% vs. 72.02 ± 14.09). In addition, total cell number was significantly (p<0.05) greater in blastocyst treated withMMP9 300 ng/ml among all treatment groups. On the other hand, there was no significant difference of ICM vs. TEratio in all groups. The expression of five out of six genes (i.e., MMP2, MMP9, IFNt, SSLP1 and HNRNPA2B1)was different among the groups. The expression of IFNt and HNRNPA2B1 genes was significantly greater in MMP9(p<0.05), but there was no difference of MMP9 expression between MMP2 and MMP9 group (p>0.05). The normalizedexpression of MMP2 and SSLP1 was greater in MMP2 than other groups (p<0.05). In conclusion, MMPs treatmentduring IVC-2 medium was remarkably effected on blastocyst developmental competence and gene expression profilesthat are related to embryo quality and implantation.
Development of New Vitrification Method for Preimplantation Mouse Embryo
A-Na Ha,Md. Fakruzzaman,Kyeong-Lim Lee,Erdan Wang,Jae-Ik Lee,Chan-Sik Min,Il-Keun Kong 韓國受精卵移植學會 2013 한국동물생명공학회지 Vol.28 No.2
The purpose of this study was attempted to new methods in mammalian embryos vitrification. This method was affected to increase of the embryo vitrification efficiency and it would be applied to the field of embryo transfer to recipient by modified loading method of embryo into 0.25 ml plastic straw. The frozen mouse embryos were carried out warmed from two different cell stages (8-cell and blastocyst, respectively) by attachment of an embryo in the vitrification straw (aV) method. All groups were cultured in M-16 medium to determine the development and survivability for 24 h, respectively. Results shown that, the survivability of two different groups were significantly different (94.8% vs. 70.9%). Total cell number was not significantly different the non-frozen blastocyst (99.7 ± 12.4) compared to the post-thaw blastocyst (94.8 ± 15.1). From the 8-cell embryo, total cell number of frozen blastocysts were significantly lower than others groups (74.7 ± 14.6, p<0.05). In the case of cell death analysis, the blastocysts from non-frozen and frozen-thawed 8-cell group were not different (0.0 ± 0.0 vs. 1.9 ± 3.1, p>0.05). However, the apoptotic nuclei of blastocyst were significantly observed the frozen-thawed group (5.4 ± 4.4) compared to non-frozen group (p<0.05). Therefore, this new method of embryos using in-straw dilution and direct transfer into other species would be more simple procedure of embryo transfer rather than step-wise dilution method and cryopreservation vessels, so we can be applied in animal as well as human embryo cryopreservation in further.