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- 저자 : Fabo Chen (검색결과 2 건)
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Fabo Chen,Jian Gao,Wenbo Li,Yi Liu,Ping Fang,Zhihang Peng 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.6
Red-fl eshed radishes (RFRs) are economically important root vegetable in the Brassicaceae family that contain high concentrationsof radish-red pigment in their fl eshy root. However, the yield of the fl eshy root is limited and germplasm resourcesfor radish improvement are lacking. Genome doubling is an eff ective and effi cient breeding tool for the rapid creation ofnew germplasm resources. Therefore, we aimed to induce tetraploidy in RFRs to improve pigment production. RFR seedswere immersed in aqueous colchicine (0, 0.01, 0.05, 0.10, 0.15, and 0.20% (w/v)) for 24 h followed by cultivation at 25 for 7 days, and those with uniform and distinctly swelled and short hypocotyls were selected as putative tetraploids. Theputative tetraploids were identifi ed by fl ow cytometry, and then we further examined the morphological and cytologicalcharacteristics of the diploid and tetraploid plants and measured gene expression via reverse transcription quantitative PCR. The results indicated that the most suitable concentration of colchicine (0.05% (w/v)) could induce tetraploidy in RFRs, witha tetraploid-induction rate as high as 34.50%. In addition, tetraploids exhibited a “gigantism” eff ect in both morphologicaland cytological traits, including swelled hypocotyls, thicker cotyledons, larger stoma, wider and longer leaves, taller plants,larger fl owers, increased pollen size, larger seed pods and seeds, and a larger taproot. Moreover, compared with diploid plants,the pigment content and yield of pigment per plant of tetraploid plants were increased by 65.11% and 216.82%, respectively. Therefore, we present a simple and effi cient method for tetraploid induction via soaking radish seeds in 0.05% (w/v) colchicinefor 24 h. The results showed that tetraploid plants not only exhibited obvious “gigantism” eff ects but also signifi cantlyenhanced accumulation of anthocyanins, and represent a practical breeding material for improving pigment yield in RFRs.
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Shi Chen,Liu Yang,Wang Jialu,Chen Fabo,Liu Zhihui,Bao Xingxian 대한조선학회 2021 International Journal of Naval Architecture and Oc Vol.13 No.1
Vortex-Induced Vibration (VIV) is a major contributor to the fatigue damage of marine risers which are often arranged in an array configuration. In addition to helical strakes and fairings, studies have been strived in searching for possible VIV suppression techniques. Inspired by giant Saguaro Cacti, flexible cylinders of different cactus-shaped cross sections were tested in a water tunnel facility, and test results showed that cactus-like body shapes reduced VIV responses of a cylinder at no cost of significant increase of drag. A series of experiments were conducted on a pair of two tandem-arranged flexible cylinders and an array of four cylinders in a square configuration to investigate the effects of wake on the dynamic responses of cylinders and the VIV mitigation effectiveness of the cactus-like body shape. Results showed that the cylinders in a square configuration, either at the upstream or downstream positions, might have larger dynamic responses than those of a single cylinder. The cactus-like body shape could mitigate VIV responses of cylinders at upstream positions in an array configuration; however, similar to helical strakes, the mitigation efficiency was reduced on downstream cylinders. Note that the cactus-like cross-sectional shape investigated was not optimized for VIV suppression. The present study indicates that the modification of the cross-sectional shape of a cylinder to a well-designed cactus-like shape may be used as an alternative technique to mitigate the VIV of marine risers.
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