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Physiological and Molecular Characterization of Cephaleuros virescens Occurring in Mango Trees
Camila Vilela Vasconcelos,Fabíola Teodoro Pereira,Elizabeth Amélia Alves Duarte,Thiago Alves Santos de Oliveira,Nei Peixoto,Daniel Diego Costa Carvalho 한국식물병리학회 2018 Plant Pathology Journal Vol.34 No.3
The objective of this work was to accomplish the isolation, molecular identification and characterizing the physiology of the causal agent of the algal spot in mango trees. For this purpose, the pathogen growth was assessed in different culture media, with subsequent observation and measurements of the filamentous cells. The molecular identification was made using mycelium obtained from leaf lesions and pure algae colonies grown in culture medium. Descriptions based on DNA sequencing indicated that the algae is Cephaleuros virescens. The algae must be isolated primarily in liquid medium for further pricking into agar medium. The highest mycelial growth average in Petri dishes occurred when the algae were grown in Trebouxia and BBM. Trebouxia enabled larger cells in the filamentous cells when compared to other culture media.
Laboratory Monitoring of Biological Therapies in Rheumatology: The Role of Immunogenicity
Maurizio Benucci,Valentina Grossi,Mariangela Manfredi,Arianna Damiani,Maria Infantino,Paolo Moscato,Luigi Cinquanta,Elisa Gremese,Barbara Tolusso,Luca Petricca,Anna Laura Fedele,Stefano Alivernini,Fab 대한진단검사의학회 2020 Annals of Laboratory Medicine Vol.40 No.2
Biological drugs, such as proteins and immunogens, are increasingly used to treat various diseases, including tumors and autoimmune diseases, and biological molecules have almost completely replaced synthetic drugs in rheumatology. Although biological treatments such as anti-tumor necrosis factor (TNF) drugs seem to be quite safe, they cause some undesirable effects, such as the onset of infections due to weakening of the immune system. Given the biological nature of these drugs, they might be recognized as extraneous; this would induce an immune reaction that neutralizes their effectiveness or lead to more serious consequences. Laboratories play a pivotal role in appropriate therapeutic management. The aim of this review was to underline the production of anti-drug antibodies during treatment with biological drugs and highlight the role of laboratories in ensuring appropriate use of these drugs.