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Physiological and Molecular Characterization of Cephaleuros virescens Occurring in Mango Trees
Vasconcelos, Camila Vilela,Pereira, Fabiola Teodoro,Duarte, Elizabeth Amelia Alves,de Oliveira, Thiago Alves Santos,Peixoto, Nei,Carvalho, Daniel Diego Costa The Korean Society of Plant Pathology 2018 Plant Pathology Journal Vol.34 No.3
The objective of this work was to accomplish the isolation, molecular identification and characterizing the physiology of the causal agent of the algal spot in mango trees. For this purpose, the pathogen growth was assessed in different culture media, with subsequent observation and measurements of the filamentous cells. The molecular identification was made using mycelium obtained from leaf lesions and pure algae colonies grown in culture medium. Descriptions based on DNA sequencing indicated that the algae is Cephaleuros virescens. The algae must be isolated primarily in liquid medium for further pricking into agar medium. The highest mycelial growth average in Petri dishes occurred when the algae were grown in Trebouxia and BBM. Trebouxia enabled larger cells in the filamentous cells when compared to other culture media.
Physiological and Molecular Characterization of Cephaleuros virescens Occurring in Mango Trees
Camila Vilela Vasconcelos,Fabíola Teodoro Pereira,Elizabeth Amélia Alves Duarte,Thiago Alves Santos de Oliveira,Nei Peixoto,Daniel Diego Costa Carvalho 한국식물병리학회 2018 Plant Pathology Journal Vol.34 No.3
The objective of this work was to accomplish the isolation, molecular identification and characterizing the physiology of the causal agent of the algal spot in mango trees. For this purpose, the pathogen growth was assessed in different culture media, with subsequent observation and measurements of the filamentous cells. The molecular identification was made using mycelium obtained from leaf lesions and pure algae colonies grown in culture medium. Descriptions based on DNA sequencing indicated that the algae is Cephaleuros virescens. The algae must be isolated primarily in liquid medium for further pricking into agar medium. The highest mycelial growth average in Petri dishes occurred when the algae were grown in Trebouxia and BBM. Trebouxia enabled larger cells in the filamentous cells when compared to other culture media.