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RISS 인기검색어
- 검색키워드
- 저자 : Eman El Gebaly (검색결과 2 건)
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( Hayam El Aggan ),( Mona Salem ),( Wessam El Gendy ),( Wageh El Gebaly ),( Eman El Gohary ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1
Aims: Chronic infection with schistosomiasis is associated with down-regulation of T-cell immune responses that require activation by innate immune cells like dendritic cells (DCs). The present work was designed to study the DC subsets (myeloid and plasmacytoid) in peripheral blood and serum levels of interleukin (IL)-12 in patients with schistosomal hepatic fibrosis (SHF) in relation to severity of liver disease and renal injury. Methods: Forty five patients with SHF and 15 healthy subjects were included in the study. The severity of liver disease was assessed using Child-Pugh classification and the Model of End Stage Liver Disease (MELD) score. Renal injury was assessed by measuring urinary albumin excretory rate (UAER) and estimated glomerular filtration rate (eGFR). The percentages of CD11c+ myeloid and CD123+ plasmacytoid DC subsets in peripheral blood were identified using 3-color flow cytometry. Quantitative determination of IL-12p70 heterodimer in serum was performed using enzyme-linked immunosorbent assay. Renal biopsies from patients with macroalbuminuria were examined by immunohistochemical technique for DCs and angiogenesis using antibodies against OX62 and factor VIII-related antigen respectively. Results: The percentages of circulating CD11c+ and CD123+ DC cells, CD11c+DC/CD123+DC ratio and serum IL-12 levels showed significant decreases in patients with SHF compared with healthy subjects (P<0.01). Renal tissues from patients with macroalbuminuria showed significant increases in the number of DCs and angiogenesis compared with normal renal tissues (P<0.01). The percentages of circulating DC subsets and serum IL-12 levels were inversely correlated with Child-Pugh score, MELD score, number of renal DCs, renal angiogenesis and UAER and were positively correlated with eGFR. The number of renal DCs showed positive correlations with UAER and renal angiogenesis and negative correlation with creatinine clearance (P<0.05). Conclusions: Dendritic cell dysfunction with a deficient IL12 production seems to play a role in the progression of liver disease and renal injury in SHF. DC-based vaccines may provide a potential new goal for immunotherapy in SHF.
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Mostafa N. Taha,Amal E. Saafan,A. Ahmedy,Eman El Gebaly,Ahmed S. Khairalla 한국미생물학회 2019 The journal of microbiology Vol.57 No.7
Quorum sensing (QS) regulates virulence factor expression in Pseudomonas aeruginosa. Inhibiting the QS-controlled virulence factors without inhibiting the growth of P. aeruginosa is a promising approach for overcoming the widespread resistance of P. aeruginosa. This study was proposed to investigate the effects of two novel synthetic peptides on the biofilm development and virulence factor production of P. aeruginosa. The tested strain was P. aeruginosa PAO1. The results indicated that both of the synthetic peptides (LIVRHK and LIVRRK) inhibited (P < 0.05) the formation of biofilms and the production of virulence factors, including pyocyanin, protease, and rhamnolipids, without inhibiting the growth of PAO1. Additionally, we detected transcriptional changes related to QS and found a significant reduction in the levels of gene expression of lasI, lasR, rhlI, and rhlR. This study demonstrates that LIVRRK and LIVRHK are novel synthetic peptides that can act as potent inhibitors of QS-regulated virulence factors in P. aeruginosa. Moreover, these synthetic peptides have potential applications in the treatment of biofilmrelated diseases. Both peptides may be able to control chronic infections and biofilm-associated problems of P. aeruginosa.
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