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Guan Yongchao,Zhang Meng,Wang Yingda,Liu Zhongzhuo,Zhao Zelin,Wang Hong,An Dingjie,Qian Aidong,Kang Yuanhuan,Sun Wuwen,Shan Xiaofeng 한국미생물학회 2022 The journal of microbiology Vol.60 No.12
Aeromonas veronii is a pathogen which can induce diseases in humans, animals and aquatic organisms, but its pathogenic mechanism and virulence factors are still elusive. In this study, we successfully constructed a mutant strain (ΔascP) by homologous recombination. The results showed that the deletion of the ascP gene significantly down-regulated the expression of associated effector proteins in A. veronii compared to its wild type. The adhesive and invasive abilities of ΔascP to EPC cells were 0.82-fold lower in contrast to the wild strain. The toxicity of ΔascP to cells was decreased by about 2.91-fold (1 h) and 1.74-fold (2 h). Furthermore, the LD50 of the mutant strain of crucian carp was reduced by 19.94-fold, and the virulence was considerably attenuated. In contrast to the wild strain, the ΔascP content in the liver and spleen was considerably lower. The titers of serum cytokines (IL-8, TNF-α, and IL-1β) in crucian carp after the infection of the ΔascP strain were considerably lower in contrast to the wild strain. Hence, the ascP gene is essential for the etiopathogenesis of A. veronii TH0426.
Bin Cheng,Dingjie Guan,Bingxue Jing 한국정밀공학회 2022 International Journal of Precision Engineering and Vol.23 No.2
Small and medium-sized manufacturing enterprises involve a lot of customized products. The degree of adaptability should be noted while improving product design and manufacturing digital and intelligent levels. This paper presents a process sequencing method of manufacturing features based on the node importance of a complex network. The method is based on the adjacency matrix and connected graph to analyze the process constraint semantics of the product model. The adjacency matrix expresses the positioning dimensions between features. The connected graph is applied to define the constraint relationships between features and aggregate the multi-dimensional process dimension chain in all directions. Based on the processing sequence of node importance in a complex network, most of process planning can be realized. The method also can make adaptive decisions for different structural parts and monitor the machining of key features. Examples verify the validity and feasibility of the proposed method.