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- 저자 : Deyu Xu (검색결과 3 건)
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Deyu Xu,Jing Zhao,Guoqiang Cao,Jinyu Wang,Qinggang Li,Ping Zheng,Shuxin Zhao,Jibin Sun 한국생물공학회 2018 Biotechnology and Bioprocess Engineering Vol.23 No.1
Phosphoenolpyruvate carboxylase (PEPC) catalyzes the carboxylation of phosphoenolpyruvate (PEP) in the presence of bicarbonate to form oxaloacetate (OAA), and it plays an important role in high-efficient production of OAA-derived metabolites such as lysine, glutamate and succinate. However, PEPCs often suffered from serious feedback inhibition by various metabolic effectors like aspartate. Here, the feedback inhibition of PEPC from Corynebacterium glutamicum was removed by adding a short terminal peptide like His-tag. The effect of His-tag location on the structure and important properties such as activity, thermostability and feedback inhibition of PEPC has been investigated. The purified untagged PEPC, Nterminal His-tagged PEPC (PEPC-N-His) and C-terminal His-tagged PEPC (PEPC-C-His) were characterized. PEPCN- His (439.71/sec/mM) showed a 1.26 and 186-fold higher catalytic efficiency than untagged PEPC (348.59/sec/mM) and PEPC-C-His (2.36/sec/mM), respectively. Both PEPCN- His and untagged PEPC were significantly inhibited by aspartate at the concentrations above 4 mM (residual activities < 10%), while PEPC-C-His was almost desensitized to aspartate within 10 mM (around 90% of residual activity). Structural analysis showed that the extension of C-terminus may cause steric hindrance for aspartate binding with enzymes, leading to the deregulation of feedback inhibition of PEPC-C-His. This study provides a deeper understanding of the effect of terminal fragments on the structure and function of PEPCs, and helps to engineer the feedback inhibition of PEPCs and structurally similar enzymes.
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Songhua Xiao,Jianwen Xu;Jun Zhao;Jianguang Liu,Jianwen Xu;Jun Zhao;Jianguang Liu,Qiaojuan Wu,Jingzhong Yu,Deyue Yu 한국식물학회 2017 Journal of Plant Biology Vol.60 No.1
In this study, we comparatively analyzed the 115 Hsp70 genes identified in Gossypium raimondii, Gossypium hirsutum and Gossypium arboreum genomes. Those Hsp70 genes unequally distributed among chromosomes in A and D genome of cotton (Gossypium spp.), and were classified into 29 groups according to the homology of them. Based on the localization information of the orthologs in Arabidopsis, the Hsp70 proteins were predicted to locate in cytosol, endoplasmic reticulum, mitochondrion or chloroplast. Homologous analysis indicated the evolutionary conservation of Hsp70 in cotton. In addition, those Hsp70 genes were differently expressed in Suyuan-045, Hai-7124 and TM-1, which were highly resistant, resistant, and sensitive to Verticillium dahliae respectively. The expressions of 26 Hsp70 genes were induced by Verticillium dahliae except for Hsp70-07/16/25/26, and the result suggested the potential involvement of them in responding to Verticillium wilt. Hsp70-08/30/31 was highly expressed in both Suyuan-045 and Hai-7124, and it was hypothesized that they might be involved in the resistance to the invasion of Verticillium dahliae. 144h after inoculation with Verticillium dahliae, the expression of Hsp70-13/14/15 was only up-regulated in Suyuan-045, and it was assumed that they might be involved in resistance to the extension of Verticillium dahliae. Further study on those Hsp70 genes would be valuable to reveal the role of them in Verticillium wilt resistance.
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Hou Junming,Ren Zhaotan,Liu Deyu,Ma Zhi,Liu Xu,Wang Wei 한국농업기계학회 2023 바이오시스템공학 Vol.48 No.2
Purpose In order to analyze the damage of castor capsule during mechanical harvesting, it is necessary to establish a suitable model and obtain the parameters of discrete element model. Therefore, the study establishes a three-compartment separation and crushing discrete element model of castor; the contact parameters and bonding parameters of the model are calibrated. The TongBi No. 16 castor variety in harvest period was taken as the research object. The sizes of whole castor capsule and single part were measured; then, the moisture content of castor in harvest period was measured. Methods In order to obtain the inherent parameters of the capsule, the elastic modulus was measured by TMS pro professional food physical property analyzer. In order to realize the three-compartment separation and fragmentation of castor, a discrete element model combining the aggregation and bonding model was established. The contact parameters are calibrated by stacking angle and formed by lifting method. The contour curve of accumulation angle is obtained with MATLAB software. The results show that the stacking angle of physical test is 26.216°. Through Plackett–Burman test, the contact parameters that have a greater impact on the stacking angle are the static friction coefficient between castors and the dynamic friction coefficient between castors. After the steepest climbing test, the value range is selected. Finally, through response surface optimization, the optimal static and dynamic friction coefficients between castors are determined to be 0.41 and 0.05, respectively. Results In order to obtain accurate bonding parameters, it is carried out through compression test. In order to reduce the damage to single compartment in the process of compression, spherical compression head is used. The results show that the maximum destructive force is 52.65 N. In conducting the simulation tests, the Box-Behnken response surface design is applied to derive the optimal bonding parameters, the normal stiffness per unit area is 9.47× 107 N/m3, shear stiffness per unit area is 4.59× 107 N/m3, normal strength is 7.92× 103 Pa, and shear strength is 1.13× 104 Pa. Finally, the stacking angle test and cone compression test are carried out by the final calibrated parameters. The relative errors between the simulation and the actual test are 1.82% and 4.10%, respectively, which indicate that the calibration results are reliable. Conclusion This study can provide the support of discrete element simulation parameters for analyzing the mechanism of castor capsule damage during mechanized harvest.
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