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- 저자 : Dengmei Nie (검색결과 1 건)
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Mingqiang Fan,Jing Bai,Tao Ding,Xiangxiang Yang,Qiaoke Si,Dengmei Nie 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.11
Purpose: This study aimed to investigate the effect of adipose-derived stem cell (ADSC) transplantation on atherosclerosis (AS)and its underlying mechanisms. Materials and Methods: In our study, rat AS model was established, and ADSCs were isolated and cultured. Atheroscleroticplaque and pathological symptoms of thoracic aorta were measured by Oil Red O staining and Hematoxylin-Eosin staining, respectively. Total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoproteincholesterol (LDL-C) levels were measured by an automatic biochemical analyzer. Expressions of vascular endothelial growth factor(VEGF), vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), aortic endothelin-1 (ET-1), interleukin-6 (IL-6), c-reactive protein (CRP), and tumor necrosis factor α (TNF-α) were measured by enzyme linked immunosorbentassay, VEGF, VCAM-1, ICAM-1, ET-1, respectively, and NF-κB p65 mRNA expressions were detected by quantitative realtimepolymerase chain reaction. Protein expressions of VEGF, VCAM-1, ICAM-1, ET-1, NF-κB p65, p-NF-κB p65, and IκBα weremeasured by western blot. Moreover, NF-κB p65 expression was measured by immunofluorescence staining. Results: ADSC transplantation alleviated the pathological symptoms of aortic AS. ADSC transplantation decreased the levels ofTC, TG, and LDL-C and increased serum HDL-C level. Meanwhile, ADSC transplantation decreased the levels of IL-6, CRP, andTNF-α in AS rats. Moreover, the expressions of VEGF, ET-1, VCAM-1, and ICAM-1 were decreased by ADSC transplantation. ADSC transplantation inhibited phosphorylation of NF-κB p65 and promoted IκBα expression in AS rats. Conclusion: Our study demonstrated that ADSC transplantation could inhibit vascular inflammatory responses and endothelialdysfunction by suppressing NF-κB pathway in AS rats.
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