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Xueli Dong,Jing Ji,Songhao Zhang,Danliu Peng,Yurong Wang,Lishuang Zhang,Jiali Li,Gang Wang 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.4
The biodegradation of straw by cellulosedegrading strains plays an important role in the disposal of agricultural waste. In this study, a Penicillium sp. strain JiTF01 that could degrade cellulose was isolated at the low temperature (10oC), and its fermentation conditions were optimized by response surface method. Strain JiTF01 was applied to degrade rice straw at 10oC. It was found that the straw degradation ratio could reach to 46.53% on the 25th day of culture with the enzyme activity of 44.30 U/mL under optimized fermentation conditions. In addition, given that strain JiTF01 showed excellent acid resistance and the cellulase produced by strain JiTF01 exhibited higher enzymatic activity and stability under acidic conditions, the uninterrupted integration process of acid pretreatment and acid fermentation of straw was then performed. The results showed that the degradation ratio of 53.61% was observed after 25 days of the fermentation at pH 3, which increased by 7.08% than that under optimized conditions. Besides, the effect of straw fermentation broth on the growth of rice seed was also investigated, and the 10-5 and 10-6 diluted fermentation broth was found that could significantly promote the growth of rice seedlings and enhance the germination rate of rice seeds under the salt stress of 100 and 200 mmol/L. The conclusions found in this article might have important implications for straw recycling in cold regions.
Deep removal of sulfur from real diesel by catalytic oxidation with halogen-free ionic liquid
Dan Liu,Jianzhou Gui,Yong-Ki Park,Shuang Yang,Yuhuan Gao,Xilai Peng,Zhaolin Sun 한국화학공학회 2012 Korean Journal of Chemical Engineering Vol.29 No.1
A halogen-free task-specific ionic liquid (TSIL) of 1-(2-carboxylic acid) ethyl-3-methylimidazolium bisulfate ([(CH2)2COOHmim] [HSO4]) is applied for deep oxidative desulfurization of real diesel as a catalyst and reaction media. The sulfur level of real diesel can be reduced from 200 to 20.5 μg/g at room temperature with small amount of ionic liquid using H2O2 as an oxidant. The used ionic liquid can be easily recycled and no obvious decrease in catalytic activity can be found after recycling five times.