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Tamboli, D.P.,Lee, D.S. Elsevier Scientific Pub. Co 2013 Journal of hazardous materials Vol.260 No.-
The development of eco-friendly and reliable processes for the synthesis of nanoparticles has attracted considerable interest in nanotechnology. In this study, an extracellular enzyme system of a newly isolated microorganism, Exiguobacterium sp. KNU1, was used for the reduction of AgNO<SUB>3</SUB> solutions to silver nanoparticles (AgNPs). The extracellularly biosynthesized AgNPs were characterized by UV-vis spectroscopy, Fourier transform infra-red spectroscopy and transmission electron microscopy. The AgNPs were approximately 30nm (range 5-50nm) in size, well-dispersed and spherical. The AgNPs were evaluated for their antimicrobial effects on different gram negative and gram positive bacteria using the minimum inhibitory concentration method. Reasonable antimicrobial activity against Salmonella typhimurium, Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus was observed. The morphological changes occurred in all the microorganisms tested. In particular, E. coli exhibited DNA fragmentation after being treated with the AgNPs. Finally, the mechanism for their bactericidal activity was proposed according to the results of scanning electron microscopy and single cell gel electrophoresis.
Avinash R. Gholave,Asif S. Tamboli,Rohit N. Mane,Ramchandra D. Gore,박재홍,Sayajirao P. Gaikwad 국립중앙과학관 2021 Journal of Asia-Pacific Biodiversity Vol.14 No.4
The Curculigo brevifolia resurrected here based on morphological, cytological, and molecular phylogeneticanalyses. Curculigo brevifolia morphologically resembles C. orchioides but differs in having bulbils atthe tip of leaves for vegetative reproduction, leaves elongated falcately shaped, beaked fruits. Cytologicalparameters, karyotype formula, total haploid genome length (THL), values of CVCL, MCA also supportedthe distinctness of C. brevifolia and C. orchioides. Phylogenetic analysis based on cpDNA data resulted inthe recognition of three clades and strongly supported the revised systematics of Hypoxidaceae. Theresurrected species nests within the Curculigo clade of Hypoxidaceae and display a close phylogeneticaffinity with newly added Curculigo species and C. orchioides.